Toxic responses of Perna viridis hepatopancreas exposed to DDT, benzo(a) pyrene and their mixture uncovered by iTRAQ-based proteomics and NMR-based metabolomics
文献类型：期刊论文
| 作者 | Song, Qinqin1,2,3; Zhou, Hailong1,2; Han, Qian1,2; Diao, Xiaoping1,2 |
| 刊名 | AQUATIC TOXICOLOGY
 |
| 出版日期 | 2017-11-01 |
| 卷号 | 192页码:48-57 |
| 关键词 | Benzo(a)Pyrene Ddt P. Viridis Hepatopancreas Itraq Metabolomics |
| ISSN号 | 0166-445X |
| DOI | 10.1016/j.aquatox.2017.09.010 |
| 文献子类 | Article |
| 英文摘要 | Dichlorodiphenyltrichloroethane (DDT) and benzo(a)pyrene (BaP) are environmental estrogens (EEs) that are ubiquitous in the marine environment. In the present study, we integrated isobaric tags for relative and absolute quantitation (iTRAQ)-based proteomic and nuclear magnetic resonance (NMR)-based metabolomic approaches to explore the toxic responses of green mussel hepatopancreas exposed to DDT (10 mu g/L), BaP (10 mu g/L) and their mixture. The metabolic responses indicated that BaP primarily disturbed energy metabolism and osmotic regulation in the hepatopancreas of the male green mussel P. viridis. Both DDT and the mixture of DDT and BaP perturbed the energy metabolism and osmotic regulation in P. viridis. The proteomic responses revealed that BaP affected the proteins involved in energy metabolism, material transformation, cytoskeleton, stress responses, reproduction and development in green mussels. DDT exposure could change the proteins involved in primary metabolism, stress responses, cytoskeleton and signal transduction. However, the mixture of DDT and BaP altered proteins associated with material and energy metabolism, stress responses, signal transduction, reproduction and development, cytoskeleton and apoptosis. This study showed that iTRAQ-based proteomic and NMR-based metabolomic approaches could effectively elucidate the essential molecular mechanism of disturbances in hepatopancreas function of green mussels exposed to environmental estrogens. |
| WOS关键词 | MUSSEL MYTILUS-GALLOPROVINCIALIS ; CLAM RUDITAPES-PHILIPPINARUM ; EARTHWORM EISENIA-FOETIDA ; OYSTER CRASSOSTREA-GIGAS ; HEAT-SHOCK PROTEINS ; GENDER-SPECIFIC RESPONSES ; 2,2',4,4'-TETRABROMODIPHENYL ETHER ; PETROCHEMICAL CONTAMINATION ; H-1-NMR SPECTROSCOPY ; SALINITY ACCLIMATION |
| WOS研究方向 | Marine & Freshwater Biology ; Toxicology |
| 语种 | 英语 |
| WOS记录号 | WOS:000415768700007 |
| 出版者 | ELSEVIER SCIENCE BV |
| 资助机构 | National Natural Science Foundation of China(41161077, 31760164) ; Specialized Research Fund for the Midwest Program of Hainan University(ZXBJH-XK002) |
| 源URL | [http://ir.yic.ac.cn/handle/133337/24046]  |
| 专题 | 烟台海岸带研究所_中科院烟台海岸带研究所知识产出 |
| 通讯作者 | Zhou, Hailong |
| 作者单位 | 1.Hainan Univ, State Key Lab Marine Resource Utilizat South Chin, Haikou 570228, Hainan, Peoples R China 2.Hainan Univ, Inst Trop Agr & Forestry, 58 Renmin Rd, Haikou 570228, Hainan, Peoples R China 3.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China |
| 推荐引用方式 GB/T 7714 | Song, Qinqin,Zhou, Hailong,Han, Qian,et al. Toxic responses of Perna viridis hepatopancreas exposed to DDT, benzo(a) pyrene and their mixture uncovered by iTRAQ-based proteomics and NMR-based metabolomics[J]. AQUATIC TOXICOLOGY,2017,192:48-57. |
| APA | Song, Qinqin,Zhou, Hailong,Han, Qian,&Diao, Xiaoping.(2017).Toxic responses of Perna viridis hepatopancreas exposed to DDT, benzo(a) pyrene and their mixture uncovered by iTRAQ-based proteomics and NMR-based metabolomics.AQUATIC TOXICOLOGY,192,48-57. |
| MLA | Song, Qinqin,et al."Toxic responses of Perna viridis hepatopancreas exposed to DDT, benzo(a) pyrene and their mixture uncovered by iTRAQ-based proteomics and NMR-based metabolomics".AQUATIC TOXICOLOGY 192(2017):48-57. |
入库方式： OAI收割
来源：烟台海岸带研究所
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