Soil microbial community analysis using denaturing gradient gel electrophoresis
文献类型：期刊论文
| 作者 | Nakatsu C. H. |
| 刊名 | Soil Science Society of America journal
 |
| 出版日期 | 2007 |
| 卷号 | 71期号:2页码:562-571 |
| 关键词 | Soil Biology Molecular Methods Soil Science Microbiology Ecology Molecular Biology Biochemical Compound Physicochemical Method Gel Electrophoresis Polymerase Chain Reaction Gene Amplification Ribosomal Rna Gene Ribosomal Dna Nucleic Acid Use Denaturing Gradient Gel Electrophoresis Analysis Method Soils Microbial Community |
| ISSN号 | 0361-5995 |
| 通讯作者 | Nakatsu C. H. |
| 英文摘要 | The most biological diversity on this planet is probably harbored in soils. Understanding the diversity and function of the microbiological component of soil poses great challenges that are being overcome by the application of molecular biological approaches. This review covers one of many approaches being used: separation of polymerase chain reaction (PCR) amplicons using denaturing gradient gel electrophoresis (DGGE). Extraction of nucleic acids directly from soils allows the examination of a community without the limitation posed by cultivation. Polymerase chain reaction provides a means to increase the numbers of a target for its detection on gels. Using the rRNA genes as a target for PCR provides phylogenetic information on populations comprising communities. Fingerprints produced by this method have allowed spatial and temporal comparisons of soil communities within and between locations or among treatments. Numerous samples can be compared because of the rapid high throughput nature of this method. Scientists now have the means to begin addressing complex ecological questions about the spatial, temporal, and nutritional interactions faced by microbes in the soil environment. |
| 公开日期 | 2010-12-27 |
| 源URL | [http://ir.yic.ac.cn/handle/133337/3004]  |
| 专题 | 烟台海岸带研究所_科研共享资源 |
| 推荐引用方式 GB/T 7714 | Nakatsu C. H.. Soil microbial community analysis using denaturing gradient gel electrophoresis[J]. Soil Science Society of America journal,2007,71(2):562-571. |
| APA | Nakatsu C. H..(2007).Soil microbial community analysis using denaturing gradient gel electrophoresis.Soil Science Society of America journal,71(2),562-571. |
| MLA | Nakatsu C. H.."Soil microbial community analysis using denaturing gradient gel electrophoresis".Soil Science Society of America journal 71.2(2007):562-571. |
入库方式： OAI收割
来源：烟台海岸带研究所
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