Suppression of zebrafish VEGF gene by cytomegalovirus promoter-driven short hairpin constructs induces vascular development defects and down regulation NRP1 expression
文献类型：期刊论文
| 作者 | Yang, ShaoLi2 ; Yan, Song3; Qin, Song2; Lin, XiuKun1
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| 刊名 | BIOLOGIA
 |
| 出版日期 | 2009-10-01 |
| 卷号 | 64期号:5页码:1025-1031 |
| 关键词 | Cmv Promoter Neuropilin-1 Short-hairpin Rna Vegf Gene Zebrafish |
| ISSN号 | 0006-3088 |
| 产权排序 | Chinese Acad Sci, Yantai Inst Coastal Zone Res Sustainable Dev;Chinese Acad Sci, Key Lab Marine Biol, Inst Oceanol;Dalian Jiaotong Univ, Coll Environm & Chem Engn |
| 通讯作者 | Lin, XK, Chinese Acad Sci, Key Lab Marine Biol, Inst Oceanol, Qingdao 266071, Peoples R China |
| 文献子类 | Article |
| 英文摘要 | The usage of RNA interference for gene knockdown in zebrafish through expression of the small interfering RNA mediators from DNA vectors has created a lot of excitement in the research community. In this work, the ability of human cytomegalovirus immediate early promoter (CMV promoter)-driven short hairpin RNA (shRNA) expression vector to induce shRNA against vascular endothelial growth factor (VEGF) gene in zebrafish was tested, and its effects on VEGF-mediated vasculogenesis and angiogenesis were evaluated. Altogether four vectors targeting various locations of VEGF gene were constructed, and pSI-V4 was proven to be the most effective one. Microinjection of pSI-V4 into the zebrafish embryos resulted in defective vascular formation and down regulation of VEGF expression. In situ hybridization analysis indicated that silencing VEGF gene expression by pSI-V4 resulted in down regulation of neuropilin-1 (NRP1), a potent VEGF receptor. Knockdown of VEGF expression by morpholino gave the same result. This provided evidence that the VEGF-mediated angiogenesis in zebrafish was in part dependent on NRP1 expression. The results contributed to a better understanding of molecular mechanisms of cardiovascular development and provided a potential promoter for making inducible knockdown in zebrafish. |
| 学科主题 | Biology |
| URL标识 | 查看原文 |
| WOS关键词 | ENDOTHELIAL GROWTH-FACTOR ; POL-II PROMOTER ; RNA INTERFERENCE ; IN-VIVO ; ANGIOGENESIS ; HEMATOPOIESIS ; NEUROPILIN-1 ; MAMMALS ; SYSTEM ; CANCER |
| WOS研究方向 | Life Sciences & Biomedicine - Other Topics |
| 语种 | 英语 |
| WOS记录号 | WOS:000269947100031 |
| 资助机构 | Key Technology Research and Development Programme of Shandong Province [2007GG2QT06020]; National Natural Science Foundation of China [40506031] |
| 公开日期 | 2011-07-05 |
| 源URL | [http://ir.yic.ac.cn/handle/133337/3478]  |
| 专题 | 烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Marine Biol, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Yantai Inst Coastal Zone Res Sustainable Dev, Yantai 264003, Peoples R China 3.Dalian Jiaotong Univ, Coll Environm & Chem Engn, Dalian 116028, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yang, ShaoLi,Yan, Song,Qin, Song,et al. Suppression of zebrafish VEGF gene by cytomegalovirus promoter-driven short hairpin constructs induces vascular development defects and down regulation NRP1 expression[J]. BIOLOGIA,2009,64(5):1025-1031. |
| APA | Yang, ShaoLi,Yan, Song,Qin, Song,&Lin, XiuKun.(2009).Suppression of zebrafish VEGF gene by cytomegalovirus promoter-driven short hairpin constructs induces vascular development defects and down regulation NRP1 expression.BIOLOGIA,64(5),1025-1031. |
| MLA | Yang, ShaoLi,et al."Suppression of zebrafish VEGF gene by cytomegalovirus promoter-driven short hairpin constructs induces vascular development defects and down regulation NRP1 expression".BIOLOGIA 64.5(2009):1025-1031. |
入库方式： OAI收割
来源：烟台海岸带研究所
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