Sugar-based growth, astaxanthin accumulation and carotenogenic transcription of heterotrophic Chlorella zofingiensis (Chlorophyta)
文献类型：期刊论文
| 作者 | Sun, Ni1,2; Wang, Yan2,3 ; Li, Yan-Tao1; Huang, Jun-Chao1; Chen, Feng1
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| 刊名 | PROCESS BIOCHEMISTRY
 |
| 出版日期 | 2008-11-01 |
| 卷号 | 43期号:11页码:1288-1292 |
| 关键词 | Astaxanthin Beta-carotene Ketolase Beta-carotene Hydroxylase Chlorella Zofingiensis Heterotrophic Sugar |
| ISSN号 | 1359-5113 |
| 产权排序 | Univ Hong Kong, Sch Biol Sci;Chinese Acad Sci, S China Sea Inst Oceanol;Chinese Acad Sci, Yantai Inst Coastal Zone Res Sustainable Dev |
| 通讯作者 | Huang, JC, Univ Hong Kong, Sch Biol Sci, Pokfulam Road, Hong Kong, Hong Kong, Peoples R China |
| 文献子类 | Article |
| 英文摘要 | The green microalga Chlorella zofingiensis can grow and produce the ketocarotenoid astaxanthin in the dark with glucose as sole carbon and energy source. In the present study, we reported that glucose, mannose, fructose, sucrose, galactose and lactose could differentially support the cell growth and astaxanthin biosynthesis. Of the sugars surveyed, glucose and mannose were the best carbon sources for the algal growth in the dark, as indicated by the relatively high specific growth rates (ca. 0.03 h(-1)) and high cell dry densities (ca. 10 g l(-1)). Furthermore, the algal cells cultured with glucose and mannose accumulated the highest amounts of astaxanthin (ca. 1 mg g(-1)), indicating a correlation between cell growth and astaxanthin formation. In addition, various sugars differentially regulated the transcription of three carotenogenic genes encoding phytoene desaturase (PDS), beta-carotene ketolase (BKT), and beta-carotene hydroxylase (CHYb) respectively. By using glucose fed-batch fermentation, high cell dry weight concentration (ca. 53 g l(-1)) and high astaxanthin production (ca. 32 mg l(-1)) were obtained, which are much higher than those ever reported in the alga. The present study suggests that C. zofingiensis is suitable for the production of natural astaxanthin on a massive scale. (C) 2008 Elsevier Ltd. All rights reserved. |
| 学科主题 | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Engineering, Chemical |
| URL标识 | 查看原文 |
| WOS关键词 | SECONDARY CAROTENOIDS ; GLUCOSE ; CULTURE ; MICROALGAE ; PLUVIALIS ; CLONING ; SYSTEM ; OXYGEN ; PLANTS ; CELLS |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Engineering |
| 语种 | 英语 |
| WOS记录号 | WOS:000260809300016 |
| 资助机构 | Research Grants Council of the Hong Kong Special Administrative Region, China [HKU 7623/05M] |
| 公开日期 | 2011-07-05 |
| 源URL | [http://ir.yic.ac.cn/handle/133337/3494]  |
| 专题 | 烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室 |
| 作者单位 | 1.Univ Hong Kong, Sch Biol Sci, Hong Kong, Hong Kong, Peoples R China 2.Chinese Acad Sci, S China Sea Inst Oceanol, Guangzhou, Guangdong, Peoples R China 3.Chinese Acad Sci, Yantai Inst Coastal Zone Res Sustainable Dev, Yantai, Peoples R China |
| 推荐引用方式 GB/T 7714 | Sun, Ni,Wang, Yan,Li, Yan-Tao,et al. Sugar-based growth, astaxanthin accumulation and carotenogenic transcription of heterotrophic Chlorella zofingiensis (Chlorophyta)[J]. PROCESS BIOCHEMISTRY,2008,43(11):1288-1292. |
| APA | Sun, Ni,Wang, Yan,Li, Yan-Tao,Huang, Jun-Chao,&Chen, Feng.(2008).Sugar-based growth, astaxanthin accumulation and carotenogenic transcription of heterotrophic Chlorella zofingiensis (Chlorophyta).PROCESS BIOCHEMISTRY,43(11),1288-1292. |
| MLA | Sun, Ni,et al."Sugar-based growth, astaxanthin accumulation and carotenogenic transcription of heterotrophic Chlorella zofingiensis (Chlorophyta)".PROCESS BIOCHEMISTRY 43.11(2008):1288-1292. |
入库方式： OAI收割
来源：烟台海岸带研究所
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