The biosynthetic pathway of carotenoids in the astaxanthin-producing green alga Chlorella zofingiensis
文献类型：期刊论文
| 作者 | Wang, Yan1,2 ; Chen, Tao2
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| 刊名 | WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
 |
| 出版日期 | 2008-12-01 |
| 卷号 | 24期号:12页码:2927-2932 |
| 关键词 | Astaxanthin Chlorella Zofingiensis Diphenylamine Zeaxanthin Glucose Stress |
| ISSN号 | 0959-3993 |
| 产权排序 | Chinese Acad Sci, Yantai Inst Coastal Zone Res Sustainable Dev;Chinese Acad Sci, S China Sea Inst Oceanol |
| 通讯作者 | Wang, Y, Chinese Acad Sci, Yantai Inst Coastal Zone Res Sustainable Dev, Yantai 264003, Shandong, Peoples R China |
| 文献子类 | Article |
| 英文摘要 | The carotenoid composition of the astaxanthin-producing green alga Chlorella zofingiensis was investigated using high-performance liquid chromatography. Astaxanthin, adonixanthin, and zeaxanthin are the major carotenoids in this alga. The pigment pattern was characterized during the accumulation period, and in response to diphenylamine (DPA), an inhibitor of carotenoid biosynthesis. An increase in zeaxanthin followed by a decrease in xanthophyll was seen after the induction of astaxanthin biosynthesis by glucose. This biphasic kinetics of zeaxanthin was parallel to the marked increase in adonixanthin (from 0 mg g(-1) to 0.21 mg g(-1)) and astaxanthin (from 0.05 mg g(-1) to 0.35 mg g(-1)) and decrease of beta-carotene (from 0.30 mg g(-1) to 0.03 mg g(-1)). More importantly, unlike the Haematococcus alga, in which there was a high beta-carotene accumulation after DPA treatment, C. zofingiensis showed an accumulation of extra zeaxanthin instead of beta-carotene after treatment of the cells with DPA. All these results observed in vivo studies corroborate the observations in vitro studies at the enzyme level that zeaxanthin is a substrate for the carotenoid oxygenase in C. zofingiensis. It is suggested that zeaxanthin might be an important intermediate and not an end product of the biosynthetic pathway of astaxanthin. Therefore, a new pathway for astaxanthin formation by C. zofingiensis, which is different from that of the other astaxanthin-producing microorganisms, is proposed. An understanding of the astaxanthin biosynthetic pathway may yield important information toward the optimization of astaxanthin production, especially for the improvement of astaxanthin through genetic manipulations. |
| 学科主题 | Biotechnology & Applied Microbiology |
| URL标识 | 查看原文 |
| WOS关键词 | HAEMATOCOCCUS-PLUVIALIS ; XANTHOPHYLL CYCLE ; CHLOROPHYTA ; INHIBITION ; MICROALGAE ; NUTRITION ; STRESS ; GENE |
| WOS研究方向 | Biotechnology & Applied Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000260319900023 |
| 资助机构 | YICCAS [A3J0708DB-010]; Sci-tech Development Grant of Shandong [2007GG2QT06020] |
| 公开日期 | 2011-07-05 |
| 源URL | [http://ir.yic.ac.cn/handle/133337/3496]  |
| 专题 | 烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Yantai Inst Coastal Zone Res Sustainable Dev, Yantai 264003, Shandong, Peoples R China 2.Chinese Acad Sci, S China Sea Inst Oceanol, Guangzhou, Guangdong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Yan,Chen, Tao. The biosynthetic pathway of carotenoids in the astaxanthin-producing green alga Chlorella zofingiensis[J]. WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY,2008,24(12):2927-2932. |
| APA | Wang, Yan,&Chen, Tao.(2008).The biosynthetic pathway of carotenoids in the astaxanthin-producing green alga Chlorella zofingiensis.WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY,24(12),2927-2932. |
| MLA | Wang, Yan,et al."The biosynthetic pathway of carotenoids in the astaxanthin-producing green alga Chlorella zofingiensis".WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY 24.12(2008):2927-2932. |
入库方式： OAI收割
来源：烟台海岸带研究所
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