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Identification and characterization of an intracellular Cu, Zn-superoxide dismutase (icCu/Zn-SOD) gene from clam Venerupis philippinarum

文献类型：期刊论文

作者Li, Chenghua1; Sun, Huili2; Chen, Aiqin1; Ning, Xuanxuan3; Wu, Huifeng1; Qin, Song1; Xue, Qinzhao1; Zhao, Jianmin1
刊名FISH & SHELLFISH IMMUNOLOGY
出版日期2010-03-01
卷号28期号:3页码:499-503
关键词Venerupis Philippinarum Iccu/zn-sod Real-time Pcr
ISSN号1050-4648
产权排序[Li, Chenghua; Chen, Aiqin; Wu, Huifeng; Qin, Song; Xue, Qinzhao; Zhao, Jianmin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China; [Sun, Huili] Chinese Acad Sci, S China Sea Inst Oceanol, Guangzhou 510301, Guangdong, Peoples R China; [Ning, Xuanxuan] SOA, Yantai Ocean Environm Monitoring Cent Stn, Yantai 264006, Peoples R China
通讯作者Zhao, JM, Chinese Acad Sci, Yantai Inst Coastal Zone Res, 17 Chunhui Rd, Yantai 264003, Peoples R China
文献子类Article
英文摘要Superoxide dismutase (SOD, EC 115.11) represents one kind of enzyme involved in scavenging the high level of reactive oxygen species (ROS) into molecular oxygen and hydrogen peroxide In the present study, the intracellular Cu/Zn-SOD gene (icCu/Zn-SOD) of Venerupis philippinarum (denoted as VpSOD) was identified from haemocytes by homology cloning and RACR approaches The full-length cDNA of VpSOD consisted of 910 nucleotides with a canonical polyadenylation signal sequence AATAAA, a polyA tail, and an open-reading frame of 465 bp encoding 154 amino acids The deduced amino acid of VpSOD shared high similarity with the icCu/Zn-SODs from other species, indicating that VpSOD should be a new member of icCu/Zn-SOD family Several highly conserved motifs including Cu, Zn binding sites (H-46, H-48, H-63, H-120 for Cu binding, and H-63, H-71, H-80, D-83 for Zn binding). intracellular disulfide bond and two Cu, Zn SOD signatures were also identified in VpSOD The temporal expression of VpSOD in haemocytes after Vibrio anguillarum challenge was recorded by quantitative real-time RT-PCR. The relative expression level of VpSOD mRNA Was Lip-regulated rapidly at 6 h post-infection and reached 18-fold of the control group. After a drastic decrease at 12 h. the expression level increased again and reached 22-fold to that in the control group at 96 h post-infection. All these results indicated that VpSOD was an acute-phase protein involved in the immune responses of V philippinarum. (C) 2009 Elsevier Ltd. All rights reserved; Superoxide dismutase (SOD, EC 115.11) represents one kind of enzyme involved in scavenging the high level of reactive oxygen species (ROS) into molecular oxygen and hydrogen peroxide In the present study, the intracellular Cu/Zn-SOD gene (icCu/Zn-SOD) of Venerupis philippinarum (denoted as VpSOD) was identified from haemocytes by homology cloning and RACR approaches The full-length cDNA of VpSOD consisted of 910 nucleotides with a canonical polyadenylation signal sequence AATAAA, a polyA tail, and an open-reading frame of 465 bp encoding 154 amino acids The deduced amino acid of VpSOD shared high similarity with the icCu/Zn-SODs from other species, indicating that VpSOD should be a new member of icCu/Zn-SOD family Several highly conserved motifs including Cu, Zn binding sites (H-46, H-48, H-63, H-120 for Cu binding, and H-63, H-71, H-80, D-83 for Zn binding). intracellular disulfide bond and two Cu, Zn SOD signatures were also identified in VpSOD The temporal expression of VpSOD in haemocytes after Vibrio anguillarum challenge was recorded by quantitative real-time RT-PCR. The relative expression level of VpSOD mRNA Was Lip-regulated rapidly at 6 h post-infection and reached 18-fold of the control group. After a drastic decrease at 12 h. the expression level increased again and reached 22-fold to that in the control group at 96 h post-infection. All these results indicated that VpSOD was an acute-phase protein involved in the immune responses of V philippinarum. (C) 2009 Elsevier Ltd. All rights reserved
学科主题Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
URL标识查看原文
WOS关键词SCALLOP ARGOPECTEN-IRRADIANS ; MESSENGER-RNA EXPRESSION ; DROSOPHILA-CATALASE GENE ; RED-BLOOD-CELLS ; MOLECULAR-CLONING ; ACATALASEMIC MUTANTS ; SIGNALING PATHWAY ; CHLAMYS-FARRERI ; CDNA CLONING ; STRESS
WOS研究方向Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
语种英语
WOS记录号WOS:000275704900013
资助机构South China Sea Institute of Oceanology, Chinese Academy of Sciences Innovation Program [kzcx2-yw-225]; NSFC [30901115]
公开日期2011-07-14
源URL[http://ir.yic.ac.cn/handle/133337/3756]  
专题烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室
烟台海岸带研究所_中科院烟台海岸带研究所知识产出
作者单位1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China
2.Chinese Acad Sci, S China Sea Inst Oceanol, Guangzhou 510301, Guangdong, Peoples R China
3.SOA, Yantai Ocean Environm Monitoring Cent Stn, Yantai 264006, Peoples R China
推荐引用方式
GB/T 7714		 Li, Chenghua,Sun, Huili,Chen, Aiqin,et al. Identification and characterization of an intracellular Cu, Zn-superoxide dismutase (icCu/Zn-SOD) gene from clam Venerupis philippinarum[J]. FISH & SHELLFISH IMMUNOLOGY,2010,28(3):499-503.
APA Li, Chenghua.,Sun, Huili.,Chen, Aiqin.,Ning, Xuanxuan.,Wu, Huifeng.,...&Zhao, Jianmin.(2010).Identification and characterization of an intracellular Cu, Zn-superoxide dismutase (icCu/Zn-SOD) gene from clam Venerupis philippinarum.FISH & SHELLFISH IMMUNOLOGY,28(3),499-503.
MLA Li, Chenghua,et al."Identification and characterization of an intracellular Cu, Zn-superoxide dismutase (icCu/Zn-SOD) gene from clam Venerupis philippinarum".FISH & SHELLFISH IMMUNOLOGY 28.3(2010):499-503.

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来源：烟台海岸带研究所

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