Allelochemical ethyl 2-methyl acetoacetate (EMA) induces oxidative damage and antioxidant responses in Phaeodactylum tricornutum
文献类型：期刊论文
| 作者 | Yang, Cui-Yun ; Liu, Su-Jing; Zhou, Shi-Wei; Wu, Hui-Feng; Yu, Jun-Bao; Xia, Chuan-Hai
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| 刊名 | PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
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| 出版日期 | 2011-05-01 |
| 卷号 | 100期号:1页码:93-103 |
| 关键词 | Phaeodactylum Tricornutum Ethyl 2-mEthyl Acetoacetate Reactive Oxygen Species Malondialdehyde Antioxidant Enzyme |
| ISSN号 | 0048-3575 |
| 产权排序 | [Yang, Cui-Yun; Liu, Su-Jing; Zhou, Shi-Wei; Wu, Hui-Feng; Yu, Jun-Bao; Xia, Chuan-Hai] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China |
| 通讯作者 | Xia, CH, Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China.chxia@yic.ac.cn |
| 文献子类 | Article |
| 英文摘要 | Ethyl 2-methyl acetoacetate (EMA) is a novel allelochemical exhibiting inhibitory effects on the growth of marine unicellular alga Phaeodactylum tricornutum (P. tricornutum). Oxidative damage and antioxidant responses in P. tricornutum were investigated to elucidate the mechanism involved in EMA inhibition on algal growth. The increase in reactive oxygen species (ROS) levels and malondialdehyde (MDA) contents following exposure to EMA suggested that alga was suffered from oxidative stress and severely damaged. The decrease in cell activity and cellular inclusions suggested that cell growth was greatly inhibited. The activities of the antioxidant enzymes including superoxide dismutase (SOD), catalase (CAT), glutathione reductase (GR), glutathione peroxide (GSH-PX) and glutathione S-transferase (GST) increased with the exposure concentration and decreased with the prolongation of exposure time. Cellular ascorbic acid (AsA) and reduced glutathione (GSH) systems were also involved in resisting oxidative stress of EMA by altering the composition of AsA and GSH pools. EMA exposure increased the contents of AsA, GSH, dehydroascorbate (DAsA) and glutathione (GSSG). However, the regeneration rate of AsA/DAsA did not change obviously between treatments and the control, while that of GSH/GSSG decreased significantly under 14 mmol/L EMA exposure on the 3rd day. These results showed that EMA-induced oxidative damage might be responsible for EMA inhibition on P. tricornutum growth and cellular antioxidant enzymes and non-enzymatic antioxidants were improved to counteract the oxidative stress. (C) 2011 Elsevier Inc. All rights reserved. |
| 学科主题 | Biochemistry & Molecular Biology ; Entomology ; Physiology |
| WOS关键词 | CYANOBACTERIUM MICROCYSTIS-AERUGINOSA ; ALGA SELENASTRUM-CAPRICORNUTUM ; ASCORBATE-GLUTATHIONE CYCLE ; LIPID-PEROXIDATION ; SUPEROXIDE-DISMUTASE ; MYRIOPHYLLUM-SPICATUM ; SIGNAL-TRANSDUCTION ; PHRAGMITES-COMMUNIS ; ENZYME-ACTIVITIES ; CUCUMBER LEAVES |
| WOS研究方向 | Biochemistry & Molecular Biology ; Entomology ; Physiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000289499900015 |
| 资助机构 | National Natural Science Foundation of China [21007088]; Research Grant for Outstanding Young Scientists of Shandong Province [BS2009CL006]; National Key Technology R&D Program of China [2008BAC32B03]; Yantai Science and Technology Bureau [2008155] |
| 公开日期 | 2011-07-22 |
| 源URL | [http://ir.yic.ac.cn/handle/133337/4868]  |
| 专题 | 烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室 烟台海岸带研究所_滨海湿地实验室 |
| 作者单位 | Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yang, Cui-Yun,Liu, Su-Jing,Zhou, Shi-Wei,et al. Allelochemical ethyl 2-methyl acetoacetate (EMA) induces oxidative damage and antioxidant responses in Phaeodactylum tricornutum[J]. PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY,2011,100(1):93-103. |
| APA | Yang, Cui-Yun,Liu, Su-Jing,Zhou, Shi-Wei,Wu, Hui-Feng,Yu, Jun-Bao,&Xia, Chuan-Hai.(2011).Allelochemical ethyl 2-methyl acetoacetate (EMA) induces oxidative damage and antioxidant responses in Phaeodactylum tricornutum.PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY,100(1),93-103. |
| MLA | Yang, Cui-Yun,et al."Allelochemical ethyl 2-methyl acetoacetate (EMA) induces oxidative damage and antioxidant responses in Phaeodactylum tricornutum".PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY 100.1(2011):93-103. |
入库方式： OAI收割
来源：烟台海岸带研究所
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