Combinational biosynthesis of phycocyanobilin using genetically-engineered Escherichia coli
文献类型：期刊论文
| 作者 | Ge, Baosheng1; Li, Yan2 ; Sun, Haixiang2; Zhang, Shuai1; Hu, Peijie1; Qin, Song3; Huang, Fang1
|
| 刊名 | BIOTECHNOLOGY LETTERS
 |
| 出版日期 | 2013-05-01 |
| 卷号 | 35期号:5页码:689-693 |
| 关键词 | Antioxidant Combinational Biosynthesis Phycocyanobilin Purification |
| ISSN号 | 0141-5492 |
| 产权排序 | [Ge, Baosheng; Zhang, Shuai; Hu, Peijie; Huang, Fang] China Univ Petr East China, Ctr Bioengn & Biotechnol, Qingdao 266580, Peoples R China; [Li, Yan; Sun, Haixiang] China Univ Petr East China, Coll Sci, Qingdao 266580, Peoples R China; [Qin, Song] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China |
| 通讯作者 | Huang, F (reprint author), China Univ Petr East China, Ctr Bioengn & Biotechnol, Qingdao 266580, Peoples R China. fhuang@upc.edu.cn |
| 文献子类 | Article |
| 英文摘要 | Genes of the key enzymes for phycocyanobilin (PCB) biosynthesis were cloned into E. coli and combinationally expressed to produce phycocyanobilin, with autologous heme as substrate. Culture conditions were optimized to achieve similar to 3 mg PCB/l. A protocol for the purification of recombinant phycocyanobilin was established using solvent extraction combined with chromatography, which resulted in a final yield of similar to 0.3 mg PCB/l with a purity > 95 %. Recombinant phycocyanobilin could scavenge hydroxyl radicals with an EC50 of 0.1 mu M.; Genes of the key enzymes for phycocyanobilin (PCB) biosynthesis were cloned into E. coli and combinationally expressed to produce phycocyanobilin, with autologous heme as substrate. Culture conditions were optimized to achieve similar to 3 mg PCB/l. A protocol for the purification of recombinant phycocyanobilin was established using solvent extraction combined with chromatography, which resulted in a final yield of similar to 0.3 mg PCB/l with a purity > 95 %. Recombinant phycocyanobilin could scavenge hydroxyl radicals with an EC50 of 0.1 mu M. |
| 学科主题 | Biotechnology & Applied Microbiology |
| URL标识 | 查看原文 |
| WOS关键词 | SUBUNIT |
| WOS研究方向 | Biotechnology & Applied Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000318510500005 |
| 资助机构 | National Natural Science Foundation of China [41176144]; Public Science and Technology Research Funds Projects of Ocean [200905021-3, 201205027]; Shandong Province Natural Science Foundation [JQ201008, ZR2012DQ015]; Ministry of Education of China [NCET-10-0815] |
| 公开日期 | 2013-08-14 |
| 源URL | [http://ir.yic.ac.cn/handle/133337/6419]  |
| 专题 | 烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室 |
| 作者单位 | 1.China Univ Petr East China, Ctr Bioengn & Biotechnol, Qingdao 266580, Peoples R China 2.China Univ Petr East China, Coll Sci, Qingdao 266580, Peoples R China 3.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ge, Baosheng,Li, Yan,Sun, Haixiang,et al. Combinational biosynthesis of phycocyanobilin using genetically-engineered Escherichia coli[J]. BIOTECHNOLOGY LETTERS,2013,35(5):689-693. |
| APA | Ge, Baosheng.,Li, Yan.,Sun, Haixiang.,Zhang, Shuai.,Hu, Peijie.,...&Huang, Fang.(2013).Combinational biosynthesis of phycocyanobilin using genetically-engineered Escherichia coli.BIOTECHNOLOGY LETTERS,35(5),689-693. |
| MLA | Ge, Baosheng,et al."Combinational biosynthesis of phycocyanobilin using genetically-engineered Escherichia coli".BIOTECHNOLOGY LETTERS 35.5(2013):689-693. |
入库方式： OAI收割
来源：烟台海岸带研究所
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