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Genetic transformation of marine cyanobacterium Synechococcus sp CC9311 (Cyanophyceae) by electroporation

文献类型：期刊论文

作者Chen Huaxin1; Lin Hanzhi1,2; Jiang Peng1; Li Fuchao1; Qin Song3
刊名CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
出版日期2013-03-01
卷号31期号:2页码:416-420
关键词Chromatic Acclimation Electroporation Genetic Transformation Synechococcus Sp Cc9311
ISSN号0254-4059
产权排序[Chen Huaxin; Lin Hanzhi; Jiang Peng; Li Fuchao] Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China; [Lin Hanzhi] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China; [Qin Song] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China
通讯作者Jiang, P (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China. jiangpeng@qdio.ac.cn
文献子类Article
英文摘要Synechococcus sp. CC9311 is a marine cyanobacterium characterized by type IV chromatic acclimation (CA). A genetic transformation system was developed as a first step to elucidate the molecular mechanism of CA. The results show that Synechococcus sp. CC9311 cells were sensitive to four commonly used antibiotics: ampicillin, kanamycin, spectinomycin, and chloramphenicol. An integrative plasmid to disrupt the putative phycoerythrin lyase gene mpeV, using a kanamycin resistance gene as selectable marker, was constructed by recombinant polymerase chain reaction. The plasmid was then transformed into Synechococcus sp. CC9311 via electroporation. High transformation efficiency was achieved at a field strength of 2 kV/cm. DNA analysis showed that mpeV was fully disrupted following challenge of the transformants with a high concentration of kanamycin. In addition, the transformants that displayed poor growth on agar SN medium could be successfully plated on agarose SN medium.; Synechococcus sp. CC9311 is a marine cyanobacterium characterized by type IV chromatic acclimation (CA). A genetic transformation system was developed as a first step to elucidate the molecular mechanism of CA. The results show that Synechococcus sp. CC9311 cells were sensitive to four commonly used antibiotics: ampicillin, kanamycin, spectinomycin, and chloramphenicol. An integrative plasmid to disrupt the putative phycoerythrin lyase gene mpeV, using a kanamycin resistance gene as selectable marker, was constructed by recombinant polymerase chain reaction. The plasmid was then transformed into Synechococcus sp. CC9311 via electroporation. High transformation efficiency was achieved at a field strength of 2 kV/cm. DNA analysis showed that mpeV was fully disrupted following challenge of the transformants with a high concentration of kanamycin. In addition, the transformants that displayed poor growth on agar SN medium could be successfully plated on agarose SN medium.
学科主题Limnology ; Oceanography
URL标识查看原文
WOS关键词CHROMATIC ADAPTATION ; MANIPULATION ; DIVERSITY ; MOTILITY ; STRAINS ; GENUS
WOS研究方向Marine & Freshwater Biology ; Oceanography
语种英语
WOS记录号WOS:000316018300018
资助机构Key Innovation Project of Institute of Oceanology, Chinese Academy of Sciences [2009-2]; Natural Science Foundation of Shandong Province [2009ZRB02542]; Foundation of Key Laboratory of Marine Bioactive Substance and Modern Analytical Techniques, SOA [MBSMAT-2010-03]; National Natural Science Foundation of China [41276164]; Natural Science Foundation of Jiangsu Province [BK2012650]
公开日期2013-08-14
源URL[http://ir.yic.ac.cn/handle/133337/6441]  
专题烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室
作者单位1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
3.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China
推荐引用方式
GB/T 7714		 Chen Huaxin,Lin Hanzhi,Jiang Peng,et al. Genetic transformation of marine cyanobacterium Synechococcus sp CC9311 (Cyanophyceae) by electroporation[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2013,31(2):416-420.
APA Chen Huaxin,Lin Hanzhi,Jiang Peng,Li Fuchao,&Qin Song.(2013).Genetic transformation of marine cyanobacterium Synechococcus sp CC9311 (Cyanophyceae) by electroporation.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,31(2),416-420.
MLA Chen Huaxin,et al."Genetic transformation of marine cyanobacterium Synechococcus sp CC9311 (Cyanophyceae) by electroporation".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 31.2(2013):416-420.

入库方式： OAI收割

来源：烟台海岸带研究所

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