Undecylprodigiosin Induced Apoptosis in P388 Cancer Cells Is Associated with Its Binding to Ribosome
文献类型：期刊论文
| 作者 | Liu, Ping1,2; Wang, Yuan-yuan1; Qi, Xin1; Gu, QianQun1; Geng, Meiyu3; Li, Jing1 |
| 刊名 | PLOS ONE
 |
| 出版日期 | 2013-06-14 |
| 卷号 | 8期号:6页码:e65381 |
| 关键词 | Cycloprodigiosin Hydrochloride h+/cl-symporter Hl-60 Cells h+-atpase In-vitro Prodigiosin Lines Differentiation Proliferation Inhibition |
| ISSN号 | 1932-6203 |
| 产权排序 | [Liu, Ping; Wang, Yuan-yuan; Qi, Xin; Gu, QianQun; Li, Jing] Ocean Univ China, Sch Med & Pharm, Minist Educ, Key Lab Marine Drugs, Qingdao, Peoples R China; [Liu, Ping] Chinese Acad Sci, Anal & Testing Ctr, Yantai Inst Coastal Zone Res, Yantai, Peoples R China; [Geng, Meiyu] Chinese Acad Sci, Shanghai Inst Mat Med, Key Lab Drug Res, Shanghai 200031, Peoples R China |
| 通讯作者 | Geng, MY (reprint author), Chinese Acad Sci, Shanghai Inst Mat Med, Key Lab Drug Res, Shanghai 200031, Peoples R China. mygeng@mail.shcnc.ac.cn ; lijing_ouc@ouc.edu.cn |
| 文献子类 | Article |
| 英文摘要 | Prodigiosins (PGs) are a family of natural red pigments with anticancer activity, and one member of the family has entered clinical phase II trials. However, the anticancer mechanisms of PGs remain largely unclear. This study was designed to investigate the molecular basis of anticancer activity of UP, a derivative of PGs, in P388 cells. By introducing pharmacological inhibitors and utilizing a variety of analytical approaches including western blotting, flow cytometry and confocal laser microscopy, we found that UP inhibited proliferation of P388 via arresting cells at G2/M phase and inducing cells apoptosis, which was related to the activation of P38, JNK rather than ERK1/2 signaling. ROS regeneration and acidification in cells appear not involved in UP induced apoptosis. Furthermore, utilizing mass spectrometry, sucrose density gradient fractionation and immunofluorescence staining, we discovered that UP was apparently located at ribosome. These results together indicate that ribosome may be the potential target of UP in cancer cells, which opened a new avenue in delineating the anticancer mechanism of PGs.; Prodigiosins (PGs) are a family of natural red pigments with anticancer activity, and one member of the family has entered clinical phase II trials. However, the anticancer mechanisms of PGs remain largely unclear. This study was designed to investigate the molecular basis of anticancer activity of UP, a derivative of PGs, in P388 cells. By introducing pharmacological inhibitors and utilizing a variety of analytical approaches including western blotting, flow cytometry and confocal laser microscopy, we found that UP inhibited proliferation of P388 via arresting cells at G2/M phase and inducing cells apoptosis, which was related to the activation of P38, JNK rather than ERK1/2 signaling. ROS regeneration and acidification in cells appear not involved in UP induced apoptosis. Furthermore, utilizing mass spectrometry, sucrose density gradient fractionation and immunofluorescence staining, we discovered that UP was apparently located at ribosome. These results together indicate that ribosome may be the potential target of UP in cancer cells, which opened a new avenue in delineating the anticancer mechanism of PGs. |
| 学科主题 | Multidisciplinary Sciences |
| URL标识 | 查看原文 |
| WOS关键词 | CYCLOPRODIGIOSIN HYDROCHLORIDE ; H+/CL-SYMPORTER ; HL-60 CELLS ; H+-ATPASE ; IN-VITRO ; PRODIGIOSIN ; LINES ; DIFFERENTIATION ; PROLIFERATION ; INHIBITION |
| WOS研究方向 | Science & Technology - Other Topics |
| 语种 | 英语 |
| WOS记录号 | WOS:000320363300018 |
| 资助机构 | National High-tech R&D Program of China [2011AA09070104]; Program for Changjiang Scholars and Innovative Research Team in University [IRT0944] |
| 公开日期 | 2013-08-14 |
| 源URL | [http://ir.yic.ac.cn/handle/133337/6475]  |
| 专题 | 烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室 |
| 作者单位 | 1.Ocean Univ China, Sch Med & Pharm, Minist Educ, Key Lab Marine Drugs, Qingdao, Peoples R China 2.Chinese Acad Sci, Anal & Testing Ctr, Yantai Inst Coastal Zone Res, Yantai, Peoples R China 3.Chinese Acad Sci, Shanghai Inst Mat Med, Key Lab Drug Res, Shanghai 200031, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Ping,Wang, Yuan-yuan,Qi, Xin,et al. Undecylprodigiosin Induced Apoptosis in P388 Cancer Cells Is Associated with Its Binding to Ribosome[J]. PLOS ONE,2013,8(6):e65381. |
| APA | Liu, Ping,Wang, Yuan-yuan,Qi, Xin,Gu, QianQun,Geng, Meiyu,&Li, Jing.(2013).Undecylprodigiosin Induced Apoptosis in P388 Cancer Cells Is Associated with Its Binding to Ribosome.PLOS ONE,8(6),e65381. |
| MLA | Liu, Ping,et al."Undecylprodigiosin Induced Apoptosis in P388 Cancer Cells Is Associated with Its Binding to Ribosome".PLOS ONE 8.6(2013):e65381. |
入库方式： OAI收割
来源：烟台海岸带研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。