Variations and evolution of polyubiquitin genes from ciliates
文献类型：期刊论文
| 作者 | Liu, Xihan1,2; Shi, Fei1; Gong, Jun1,3
|
| 刊名 | EUROPEAN JOURNAL OF PROTISTOLOGY
 |
| 出版日期 | 2013 |
| 卷号 | 49期号:1页码:40-49 |
| 关键词 | Ciliates Ubiquitin Polyubiquitin Gene Molecular Evolution Phylogenetics |
| ISSN号 | 0932-4739 |
| 产权排序 | [Liu, Xihan; Shi, Fei; Gong, Jun] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China; [Liu, Xihan] Ocean Univ China, Lab Protozool, Qingdao 266003, Peoples R China; [Gong, Jun] S China Normal Univ, Coll Life Sci, Guangzhou 510631, Guangdong, Peoples R China |
| 通讯作者 | Gong, J (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China. jgong@yic.ac.cn |
| 文献子类 | Article |
| 英文摘要 | Polyubiquitin genes from seven ciliate species were amplified, cloned and sequenced. It is estimated that Strombidium sulcatum, Euplotes vannus, E. rariseta and Anteholosticha manca have a polyubiquitin gene of 3 repeats, and A. parawarreni, Paramecium caudatum and Pseudokeronopsis flava 4 repeats. The newly obtained ubiquitins mostly differ from that of humans by 1-5 residues in amino acid sequences. A neighbor-joining tree constructed based on monomeric ubiquitin genes supports the monophyly of an assemblage comprising the litostomateans and some oligohymenophoreans, but not the class Spirotrichea. The monomers from the same species are generally placed together and highly supported for the class Litostomatea, the genera Paramecium and Icthyophthirius, but not for other species. The non-synonymous/synonymous rate ratio (dN/dS) at the protein level are less than 1, and the synonymous nucleotide differences per synonymous site (p(S)) from intraspecific comparisons are fairly high (0.02-0.72). These results indicate that ciliates have not only the conserved, but also some quite divergent, polyubiquitin genes and confirm that the polyubiquitin genes in ciliates evolve according to the birth-and-death mode of evolution under strong purifying selection. (C) 2012 Elsevier GmbH. All rights reserved.; Polyubiquitin genes from seven ciliate species were amplified, cloned and sequenced. It is estimated that Strombidium sulcatum, Euplotes vannus, E. rariseta and Anteholosticha manca have a polyubiquitin gene of 3 repeats, and A. parawarreni, Paramecium caudatum and Pseudokeronopsis flava 4 repeats. The newly obtained ubiquitins mostly differ from that of humans by 1-5 residues in amino acid sequences. A neighbor-joining tree constructed based on monomeric ubiquitin genes supports the monophyly of an assemblage comprising the litostomateans and some oligohymenophoreans, but not the class Spirotrichea. The monomers from the same species are generally placed together and highly supported for the class Litostomatea, the genera Paramecium and Icthyophthirius, but not for other species. The non-synonymous/synonymous rate ratio (dN/dS) at the protein level are less than 1, and the synonymous nucleotide differences per synonymous site (p(S)) from intraspecific comparisons are fairly high (0.02-0.72). These results indicate that ciliates have not only the conserved, but also some quite divergent, polyubiquitin genes and confirm that the polyubiquitin genes in ciliates evolve according to the birth-and-death mode of evolution under strong purifying selection. (C) 2012 Elsevier GmbH. All rights reserved. |
| 学科主题 | Microbiology |
| URL标识 | 查看原文 |
| WOS关键词 | UBIQUITIN GENES ; DEATH EVOLUTION ; PURIFYING SELECTION ; GENOME SEQUENCE ; TETRAHYMENA-PYRIFORMIS ; PHYLOGENETIC ANALYSIS ; ARABIDOPSIS-THALIANA ; STRUCTURAL FEATURES ; MOLECULAR PHYLOGENY ; MAXIMUM-LIKELIHOOD |
| WOS研究方向 | Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000316831900004 |
| 资助机构 | FANEDD [2007B27]; Natural Science Foundation of China [40976099]; One Hundred Talent Program of CAS |
| 公开日期 | 2013-08-14 |
| 源URL | [http://ir.yic.ac.cn/handle/133337/6479]  |
| 专题 | 烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China 2.Ocean Univ China, Lab Protozool, Qingdao 266003, Peoples R China 3.S China Normal Univ, Coll Life Sci, Guangzhou 510631, Guangdong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Xihan,Shi, Fei,Gong, Jun. Variations and evolution of polyubiquitin genes from ciliates[J]. EUROPEAN JOURNAL OF PROTISTOLOGY,2013,49(1):40-49. |
| APA | Liu, Xihan,Shi, Fei,&Gong, Jun.(2013).Variations and evolution of polyubiquitin genes from ciliates.EUROPEAN JOURNAL OF PROTISTOLOGY,49(1),40-49. |
| MLA | Liu, Xihan,et al."Variations and evolution of polyubiquitin genes from ciliates".EUROPEAN JOURNAL OF PROTISTOLOGY 49.1(2013):40-49. |
入库方式： OAI收割
来源：烟台海岸带研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。