Current-Driven Ion Fluxes of Polymeric Membrane Ion-Selective Electrode for Potentiometric Biosensing
文献类型：期刊论文
| 作者 | Ding, Jiawang1,2,3 ; Qin, Wei1
|
| 刊名 | JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
 |
| 出版日期 | 2009-10-21 |
| 卷号 | 131期号:41页码:14640-+ |
| 关键词 | Uniform Concentration Profiles Current Pulse Method Detection Limits Sensors Polarization Coefficients Titrations |
| 产权排序 | Chinese Acad Sci, Key Lab Coastal Environm Proc, Yantai Inst Coastal Zone Res Sustainable Dev;Chinese Acad Sci, S China Sea Inst Oceanol;Chinese Acad Sci, Grad Univ |
| 通讯作者 | Qin, W, Chinese Acad Sci, Key Lab Coastal Environm Proc, Yantai Inst Coastal Zone Res Sustainable Dev, Yantai 264003, Peoples R China |
| 文献子类 | Article |
| 英文摘要 | A polymeric membrane ion-selective electrode (ISE) for rapid potentiometric biosensing has been developed. Butyrylcholinesterase, as a target enzyme, is immobilized on the surface of the polymeric membrane. A macro-command-controlled procedure for switching between the potentiostatic and galvanostatic steps has been designed to provide a current-driven release of the butyrylcholine substrate for in situ biosensing of the enzyme and its inhibitors. This system has the flexibility of trapping substrate ions in the ISE inner filling solution and provides rapid, continuous, and reproducible measurements of enzymes and other bioanalytes involved in enzymatic systems.; A polymeric membrane ion-selective electrode (ISE) for rapid potentiometric biosensing has been developed. Butyrylcholinesterase, as a target enzyme, is immobilized on the surface of the polymeric membrane. A macro-command-controlled procedure for switching between the potentiostatic and galvanostatic steps has been designed to provide a current-driven release of the butyrylcholine substrate for in situ biosensing of the enzyme and its inhibitors. This system has the flexibility of trapping substrate ions in the ISE inner filling solution and provides rapid, continuous, and reproducible measurements of enzymes and other bioanalytes involved in enzymatic systems. |
| 学科主题 | Chemistry ; Multidisciplinary |
| WOS关键词 | UNIFORM CONCENTRATION PROFILES ; CURRENT PULSE METHOD ; DETECTION LIMITS ; SENSORS ; POLARIZATION ; COEFFICIENTS ; TITRATIONS |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000271271800021 |
| 资助机构 | Chinese Academy of Sciences [KZCX2-YW-410]; NSFC [40776058]; MOST [2007AA09Z103]; Taishan Scholar Program ; Natural Science Foundation of Shandong Province [JQ200814] |
| 源URL | [http://ir.yic.ac.cn/handle/133337/3432]  |
| 专题 | 烟台海岸带研究所_近岸生态与环境实验室 烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Coastal Environm Proc, Yantai Inst Coastal Zone Res Sustainable Dev, Yantai 264003, Peoples R China 2.Chinese Acad Sci, S China Sea Inst Oceanol, Guangzhou 510301, Guangdong, Peoples R China 3.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ding, Jiawang,Qin, Wei. Current-Driven Ion Fluxes of Polymeric Membrane Ion-Selective Electrode for Potentiometric Biosensing[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,2009,131(41):14640-+. |
| APA | Ding, Jiawang,&Qin, Wei.(2009).Current-Driven Ion Fluxes of Polymeric Membrane Ion-Selective Electrode for Potentiometric Biosensing.JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,131(41),14640-+. |
| MLA | Ding, Jiawang,et al."Current-Driven Ion Fluxes of Polymeric Membrane Ion-Selective Electrode for Potentiometric Biosensing".JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 131.41(2009):14640-+. |
入库方式： OAI收割
来源：烟台海岸带研究所
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