Changes of cytosolic Ca2+ fluorescence intensity and plasma membrane calcium channels of maize root tip cells under osmotic stress
文献类型：期刊论文
| 作者 | Liu, Zihui2; Ma, Zhenyu2; Guo, Xiulin2; Shao, Hongbo1,3,4; Cui, Qiuhua2; Song, Weiyi5 |
| 刊名 | PLANT PHYSIOLOGY AND BIOCHEMISTRY
 |
| 出版日期 | 2010-10-01 |
| 卷号 | 48期号:10-11页码:860-865 |
| 关键词 | Abscisic Acid Maize Root Calcium Channel Confocal Membrane-clamp Calcium Signal |
| ISSN号 | 0981-9428 |
| 产权排序 | [Shao, Hongbo] Qingdao Univ Sci & Technol, Inst Life Sci, Qingdao 266042, Peoples R China; [Liu, Zihui; Ma, Zhenyu; Guo, Xiulin; Cui, Qiuhua] Hebei Acad Agr & Forestry Sci, Plant Genet Engn Ctr Hebei Prov, Inst Genet & Physiol, Shijiazhuang, Peoples R China; [Shao, Hongbo] Chinese Acad Sci, State Key Lab Soil Eros & Dryland Farming Loess P, Inst Soil & Water Conservat, Yangling, Peoples R China; [Shao, Hongbo] Chinese Acad Sci, CAS Key Lab Coastal Environm Proc, Shandong Prov Key Lab Coastal Environm Proc, Yantai Inst Costal Zone Res, Yantai, Shandong, Peoples R China; [Song, Weiyi] Shangqiu Normal Univ, Dept Biol, Shangqiu, Peoples R China |
| 通讯作者 | Shao, HB, Qingdao Univ Sci & Technol, Inst Life Sci, Zhengzhou Rd 53, Qingdao 266042, Peoples R China |
| 文献子类 | Article |
| 英文摘要 | The changes of cytosolic Ca2+ fluorescence intensity and the activities of calcium channel of primary maize root tip cells induced by PEG6000 or abscisic acid(ABA) were studied by both confocal techniques and the whole-cell patch clamping in this study. The Ca2+ fluorescence intensity increased while treated with PEG or ABA within 10 min, illuminating that Ca2+ participated in the process of ABA signal transduction. For further proving the mechanism and origin of cytosolic Ca2+ increase induced by PEG treatments, N,N,N',N'-tetraacetic acid (EGTA), Verapamil (VP) and Trifluoperazine (TFP) were added to the PEG solution in the experiments separately. The results showed that Ca2+ fluorescence intensity induced by PEG was suppressed by both EGTA and VP obviously in the root tip cells. The Ca2+ fluorescence intensity of plants changed after the addition of CaM inhibitor TFP while subjected to osmotic stress, which seemed to show that CaM participated in the process of signal transduction of osmotic stress too. The mechanism about it is unknown today. Further, a hyperpolarization-activated calcium permeable channel was recorded in plasma membrane of maize root tip cells. The Ca2+ current (I-Ca) intensity increased remarkably after PEG treatment, and the open voltage of the calcium conductance increased. Similar changes could be observed after ABA treatment, but the channel opened earlier and the current intensity was stronger than that of PEG treatment. The activation of calcium channel initiated by PEG strongly was inhibited by EGTA, VP or TFP respectively. The results revealed that Ca2+ participated in the signals transduction process of osmotic stress, and the cytosolic free Ca2+ increase by osmotic stress mainly came from the extracellular, and some came from the release of cytoplasmic calcium pool. (C) 2010 Elsevier Masson SAS. All rights reserved.; The changes of cytosolic Ca2+ fluorescence intensity and the activities of calcium channel of primary maize root tip cells induced by PEG6000 or abscisic acid(ABA) were studied by both confocal techniques and the whole-cell patch clamping in this study. The Ca2+ fluorescence intensity increased while treated with PEG or ABA within 10 min, illuminating that Ca2+ participated in the process of ABA signal transduction. For further proving the mechanism and origin of cytosolic Ca2+ increase induced by PEG treatments, N,N,N',N'-tetraacetic acid (EGTA), Verapamil (VP) and Trifluoperazine (TFP) were added to the PEG solution in the experiments separately. The results showed that Ca2+ fluorescence intensity induced by PEG was suppressed by both EGTA and VP obviously in the root tip cells. The Ca2+ fluorescence intensity of plants changed after the addition of CaM inhibitor TFP while subjected to osmotic stress, which seemed to show that CaM participated in the process of signal transduction of osmotic stress too. The mechanism about it is unknown today. Further, a hyperpolarization-activated calcium permeable channel was recorded in plasma membrane of maize root tip cells. The Ca2+ current (I-Ca) intensity increased remarkably after PEG treatment, and the open voltage of the calcium conductance increased. Similar changes could be observed after ABA treatment, but the channel opened earlier and the current intensity was stronger than that of PEG treatment. The activation of calcium channel initiated by PEG strongly was inhibited by EGTA, VP or TFP respectively. The results revealed that Ca2+ participated in the signals transduction process of osmotic stress, and the cytosolic free Ca2+ increase by osmotic stress mainly came from the extracellular, and some came from the release of cytoplasmic calcium pool. (C) 2010 Elsevier Masson SAS. All rights reserved. |
| 学科主题 | Plant Sciences |
| URL标识 | 查看原文 |
| WOS关键词 | MESOPHYLL-CELLS ; ION-CHANNEL ; GUARD ; RESPONSES ; DROUGHT ; DEFENSE ; PLANTS ; SALT |
| WOS研究方向 | Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000284342800008 |
| 资助机构 | Key Basic Research, China [C2007000994]; Natural Science Foundation of Hebei Province, China [C2007000994]; National Science & Technology Supporting Project, China [2007BAD69B01]; Chinese Academy of Sciences (CAS) [KZCX2-YW-JC203, 2009Y2B211] |
| 公开日期 | 2011-07-14 |
| 源URL | [http://ir.yic.ac.cn/handle/133337/3682]  |
| 专题 | 烟台海岸带研究所_滨海湿地实验室 |
| 作者单位 | 1.Qingdao Univ Sci & Technol, Inst Life Sci, Qingdao 266042, Peoples R China 2.Hebei Acad Agr & Forestry Sci, Plant Genet Engn Ctr Hebei Prov, Inst Genet & Physiol, Shijiazhuang, Peoples R China 3.Chinese Acad Sci, State Key Lab Soil Eros & Dryland Farming Loess P, Inst Soil & Water Conservat, Yangling, Peoples R China 4.Chinese Acad Sci, CAS Key Lab Coastal Environm Proc, Shandong Prov Key Lab Coastal Environm Proc, Yantai Inst Costal Zone Res, Yantai, Shandong, Peoples R China 5.Shangqiu Normal Univ, Dept Biol, Shangqiu, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Zihui,Ma, Zhenyu,Guo, Xiulin,et al. Changes of cytosolic Ca2+ fluorescence intensity and plasma membrane calcium channels of maize root tip cells under osmotic stress[J]. PLANT PHYSIOLOGY AND BIOCHEMISTRY,2010,48(10-11):860-865. |
| APA | Liu, Zihui,Ma, Zhenyu,Guo, Xiulin,Shao, Hongbo,Cui, Qiuhua,&Song, Weiyi.(2010).Changes of cytosolic Ca2+ fluorescence intensity and plasma membrane calcium channels of maize root tip cells under osmotic stress.PLANT PHYSIOLOGY AND BIOCHEMISTRY,48(10-11),860-865. |
| MLA | Liu, Zihui,et al."Changes of cytosolic Ca2+ fluorescence intensity and plasma membrane calcium channels of maize root tip cells under osmotic stress".PLANT PHYSIOLOGY AND BIOCHEMISTRY 48.10-11(2010):860-865. |
入库方式： OAI收割
来源：烟台海岸带研究所
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