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The first molluscan TCTP in Venerupis philippinarum: Molecular cloning and expression analysis

文献类型：期刊论文

作者Li, Chenghua1; Qiu, Limei2; Ning, Xuanxuan3; Chen, Aiqin1; Qin, Song1; Wu, Huifeng1; Zhao, Jianmin1
刊名FISH & SHELLFISH IMMUNOLOGY
出版日期2010-09-01
卷号29期号:3页码:530-533
关键词Venerupis Philippinarum Tctp Tissues Expression Time-course Expression
ISSN号1050-4648
产权排序[Li, Chenghua; Chen, Aiqin; Qin, Song; Wu, Huifeng; Zhao, Jianmin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China; [Qiu, Limei] Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China; [Ning, Xuanxuan] SOA, Yantai Ocean Environm Monitoring Cent Stn, Yantai 264006, Peoples R China
通讯作者Zhao, JM, Chinese Acad Sci, Yantai Inst Coastal Zone Res, 17 Chunhui Rd, Yantai 264003, Peoples R China
文献子类Article
英文摘要Translationally controlled tumor protein (TCTP) is one of the abundant and ubiquitously expressed proteins in metazoans In the present study, the first molluscan TCTP (denoted as VpTCTP) was identified from Venerupis philippinarum haemocytes by EST and RACE approaches The full-length cDNA of VpTCTP consisted of 1148 nucleotides with an open-reading frame of 555 bp encoding 184 amino acids The deduced amino acid sequence of VpTCTP shared high similarity with TCTPs from other species, indicating that VpTCTP should be a new member of TCTP family Several highly conserved motifs, including 5'terminal ologopyrimidine (5'TOP) starting sequence and rich AU and AUUT elements in 3'UTR, were also identified in VpTCTP The tissue and temporal expression of VpTCTP after Vi boo anguillarum challenge was recorded by quantitative real-time RT-PCR. VpTCTP transcript could be detected in all examined tissues with the highest expression level in haemocytes and the lowest in hepatopancreas Concerning the time-course expression in haemocytes, the relative expression of VpTCTP mRNA was down-regulated sharply from 6 h to 12 h post-infection. Then, the expression level was obviously up-regulated and reached 3.4-fold to that in the control group at 48 h post challenge As time progressed, the expression of VpTCTP recovered to the original level at 96 h. All these results indicated that VpTCTP was an acute-phase protein involved in the Immune response of V philippinarum (C) 2010 Elsevier Ltd. All rights reserved.; Translationally controlled tumor protein (TCTP) is one of the abundant and ubiquitously expressed proteins in metazoans In the present study, the first molluscan TCTP (denoted as VpTCTP) was identified from Venerupis philippinarum haemocytes by EST and RACE approaches The full-length cDNA of VpTCTP consisted of 1148 nucleotides with an open-reading frame of 555 bp encoding 184 amino acids The deduced amino acid sequence of VpTCTP shared high similarity with TCTPs from other species, indicating that VpTCTP should be a new member of TCTP family Several highly conserved motifs, including 5'terminal ologopyrimidine (5'TOP) starting sequence and rich AU and AUUT elements in 3'UTR, were also identified in VpTCTP The tissue and temporal expression of VpTCTP after Vi boo anguillarum challenge was recorded by quantitative real-time RT-PCR. VpTCTP transcript could be detected in all examined tissues with the highest expression level in haemocytes and the lowest in hepatopancreas Concerning the time-course expression in haemocytes, the relative expression of VpTCTP mRNA was down-regulated sharply from 6 h to 12 h post-infection. Then, the expression level was obviously up-regulated and reached 3.4-fold to that in the control group at 48 h post challenge As time progressed, the expression of VpTCTP recovered to the original level at 96 h. All these results indicated that VpTCTP was an acute-phase protein involved in the Immune response of V philippinarum (C) 2010 Elsevier Ltd. All rights reserved.
学科主题Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
URL标识查看原文
WOS关键词CONTROLLED TUMOR PROTEIN ; IMMUNE-RESPONSE ; WHITE SHRIMP ; LITOPENAEUS-VANNAMEI ; GENE-EXPRESSION ; JAPONICUS ; GROWTH
WOS研究方向Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
语种英语
WOS记录号WOS:000280513700020
公开日期2011-07-14
源URL[http://ir.yic.ac.cn/handle/133337/3800]  
专题烟台海岸带研究所_污染过程与控制实验室
烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室
作者单位1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China
2.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
3.SOA, Yantai Ocean Environm Monitoring Cent Stn, Yantai 264006, Peoples R China
推荐引用方式
GB/T 7714		 Li, Chenghua,Qiu, Limei,Ning, Xuanxuan,et al. The first molluscan TCTP in Venerupis philippinarum: Molecular cloning and expression analysis[J]. FISH & SHELLFISH IMMUNOLOGY,2010,29(3):530-533.
APA Li, Chenghua.,Qiu, Limei.,Ning, Xuanxuan.,Chen, Aiqin.,Qin, Song.,...&Zhao, Jianmin.(2010).The first molluscan TCTP in Venerupis philippinarum: Molecular cloning and expression analysis.FISH & SHELLFISH IMMUNOLOGY,29(3),530-533.
MLA Li, Chenghua,et al."The first molluscan TCTP in Venerupis philippinarum: Molecular cloning and expression analysis".FISH & SHELLFISH IMMUNOLOGY 29.3(2010):530-533.

入库方式： OAI收割

来源：烟台海岸带研究所

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