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Serine/Threonine Protein Kinase SpkG Is a Candidate for High Salt Resistance in the Unicellular Cyanobacterium Synechocystis sp PCC 6803

文献类型：期刊论文

作者Liang, Chengwei1,2; Zhang, Xiaowen2,3; Chi, Xiaoyuan2; Guan, Xiangyu4; Li, Youxun2; Qin, Song2; Shao, Hong Bo5
刊名PLOS ONE
出版日期2011-05-26
卷号6期号:5页码:e18718
关键词Sp Pcc-6803 Signal-transduction Genes Stress Expression Bacteria Competence Perception Systems Xanthus
ISSN号1932-6203
产权排序[Liang, Chengwei] Qingdao Univ Sci & Technol, Qingdao, Peoples R China; [Liang, Chengwei; Zhang, Xiaowen; Chi, Xiaoyuan; Li, Youxun; Qin, Song] Chinese Acad Sci, Inst Oceanol, Qingdao, Peoples R China; [Zhang, Xiaowen] Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao, Peoples R China; [Guan, Xiangyu] Ocean Univ China, Qingdao, Peoples R China; [Shao, Hong Bo] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai, Peoples R China
通讯作者Liang, CW, Qingdao Univ Sci & Technol, Qingdao, Peoples R China.sqin@yic.ac.cn
文献子类Article
英文摘要Background: Seven serine/threonine kinase genes have been predicted in unicellular cyanobacterium Synechocystis sp. PCC6803. SpkA and SpkB were shown to be required for cell motility and SpkE has no kinase activity. There is no report whether the other four STKs are involved in stress-mediated signaling in Synechocystis PCC6803. Methodology/Principal Findings: In this paper, we examined differential expression of the other four serine/threonine kinases, SpkC, SpkD, SpkF and SpkG, at seven different stress conditions. The transcriptional level was up-regulated of spkG and down-regulated of spkC under high salt stress condition. Two spk deletion mutants, Delta spkC and Delta spkG, were constructed and their growth characteristic were examined compared to the wild strain. The wild strain and Delta spkC mutant were not affected under high salt stress conditions. In contrast, growth of spkG mutant was completely impaired. To further confirm the function of spkG, we also examined the effect of mutation of spkG on the expression of salt stress-inducible genes. We compared genome-wide patterns of transcription between wild-type Synechocystis sp. PCC6803 and cells with a mutation in the SpkG with DNA microarray analysis. Conclusion: In this study, we first study the spkG gene as sensor of high salt signal. We consider that SpkG play essential roles in Synechocystis sp. for sensing the high salt signal directly, rather than mediating signals among other kinases. Our microarray experiment may help select relatively significant genes for further research on mechanisms of signal transduction of Synechocystis sp. PCC6803 under high salt stress.; Background: Seven serine/threonine kinase genes have been predicted in unicellular cyanobacterium Synechocystis sp. PCC6803. SpkA and SpkB were shown to be required for cell motility and SpkE has no kinase activity. There is no report whether the other four STKs are involved in stress-mediated signaling in Synechocystis PCC6803.
学科主题Biology
WOS关键词SP PCC-6803 ; SIGNAL-TRANSDUCTION ; GENES ; STRESS ; EXPRESSION ; BACTERIA ; COMPETENCE ; PERCEPTION ; SYSTEMS ; XANTHUS
WOS研究方向Science & Technology - Other Topics
语种英语
WOS记录号WOS:000291052200008
资助机构Chinese Academy of Sciences [KZCX2-YW-223]; National Natural Science Foundation of China [40803023]; Key Program of Natural Science Foundation of Shandong Province [ZR2010DZ001]; Talents Foundation of Chinese Academy of Sciences [AJ0809BX-036]
公开日期2011-07-22
源URL[http://ir.yic.ac.cn/handle/133337/4830]  
专题烟台海岸带研究所_滨海湿地实验室
烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室
作者单位1.Qingdao Univ Sci & Technol, Qingdao, Peoples R China
2.Chinese Acad Sci, Inst Oceanol, Qingdao, Peoples R China
3.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao, Peoples R China
4.Ocean Univ China, Qingdao, Peoples R China
5.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai, Peoples R China
推荐引用方式
GB/T 7714		 Liang, Chengwei,Zhang, Xiaowen,Chi, Xiaoyuan,et al. Serine/Threonine Protein Kinase SpkG Is a Candidate for High Salt Resistance in the Unicellular Cyanobacterium Synechocystis sp PCC 6803[J]. PLOS ONE,2011,6(5):e18718.
APA Liang, Chengwei.,Zhang, Xiaowen.,Chi, Xiaoyuan.,Guan, Xiangyu.,Li, Youxun.,...&Shao, Hong Bo.(2011).Serine/Threonine Protein Kinase SpkG Is a Candidate for High Salt Resistance in the Unicellular Cyanobacterium Synechocystis sp PCC 6803.PLOS ONE,6(5),e18718.
MLA Liang, Chengwei,et al."Serine/Threonine Protein Kinase SpkG Is a Candidate for High Salt Resistance in the Unicellular Cyanobacterium Synechocystis sp PCC 6803".PLOS ONE 6.5(2011):e18718.

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来源：烟台海岸带研究所

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