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Headspace solid-phase microextraction with on-fiber derivatization for the determination of aldehydes in algae by gas chromatography-mass spectrometry

文献类型：期刊论文

作者Ma, Jiping3; Xiao, Ronghui3; Li, Jinhua1,2; Li, Jie3; Shi, Benzhang3; Liang, Yanjuan3; Lu, Wenhui1,2,3; Chen, Lingxin1,2
刊名JOURNAL OF SEPARATION SCIENCE
出版日期2011-06-01
卷号34期号:12页码:1477-1483
关键词Aldehyde Alga Derivatization Gc-ms Hs-spme
ISSN号1615-9306
产权排序[Li, Jinhua; Lu, Wenhui; Chen, Lingxin] Chinese Acad Sci, Key Lab Coastal Zone Environm Proc, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai 264003, Shandong, Peoples R China; [Li, Jinhua; Lu, Wenhui; Chen, Lingxin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Shandong, Peoples R China; [Ma, Jiping; Xiao, Ronghui; Li, Jie; Shi, Benzhang; Liang, Yanjuan; Lu, Wenhui] Qingdao Technol Univ, Inst Environm & Municipal Engn, Key Lab Environm Engn Shandong Prov, Qingdao, Peoples R China
通讯作者Chen, LX, Chinese Acad Sci, Key Lab Coastal Zone Environm Proc, Shandong Prov Key Lab Coastal Zone Environm Proc, Chunhui Rd 17, Yantai 264003, Shandong, Peoples R China.majiping2001@yahoo.com.cn ; lxchen@yic.ac.cn
文献子类Article
英文摘要A simple, fast, sensitive and cost-effective method based on headspace solid-phase microextraction (HS-SPME) with on-fiber derivatization coupled with gas chromatography-mass spectrometry was developed for the determination of six typical aldehydes, 2E-hexenal, heptanal, 2E-heptenal, 2E,4E-heptadienal, 2E-decenal and 2E,4E-decadienal in laboratory algae cultures. As derivatization reagent, O-2,3,4,5,6-(pentafluorobenzyl) hydroxylamine hydrochloride, was loaded onto the poly(dimethylsiloxane)/divinylbenzene fiber for aldehydes on-fiber derivatization prior to HS-SPME. Various influence factors of extraction efficiency were systematically investigated. Under optimized extraction conditions, excellent method performances for all the six aldehydes were attained, such as satisfactory extraction recoveries ranging from 67.1 to 117%, with the precision (relative standard deviation) within 5.3-11.1%, and low detection limits in the range of 0.026-0.044 mu g/L. The validated method was successfully applied for the analysis of the aldehydes in two diatoms (Skeletonema costatum and Chaetoceros muelleri), two pyrrophytas (Prorocentrum micans and Scrippsiella trochoidea) and Calanus sinicus eggs (feeding on the two diatoms above).; A simple, fast, sensitive and cost-effective method based on headspace solid-phase microextraction (HS-SPME) with on-fiber derivatization coupled with gas chromatography-mass spectrometry was developed for the determination of six typical aldehydes, 2E-hexenal, heptanal, 2E-heptenal, 2E,4E-heptadienal, 2E-decenal and 2E,4E-decadienal in laboratory algae cultures. As derivatization reagent, O-2,3,4,5,6-(pentafluorobenzyl) hydroxylamine hydrochloride, was loaded onto the poly(dimethylsiloxane)/divinylbenzene fiber for aldehydes on-fiber derivatization prior to HS-SPME. Various influence factors of extraction efficiency were systematically investigated. Under optimized extraction conditions, excellent method performances for all the six aldehydes were attained, such as satisfactory extraction recoveries ranging from 67.1 to 117%, with the precision (relative standard deviation) within 5.3-11.1%, and low detection limits in the range of 0.026-0.044 mu g/L. The validated method was successfully applied for the analysis of the aldehydes in two diatoms (Skeletonema costatum and Chaetoceros muelleri), two pyrrophytas (Prorocentrum micans and Scrippsiella trochoidea) and Calanus sinicus eggs (feeding on the two diatoms above).
学科主题Chemistry, Analytical
WOS关键词SINGLE-DROP MICROEXTRACTION ; ALPHA,BETA,GAMMA,DELTA-UNSATURATED ALDEHYDES ; CHEMICAL DEFENSE ; HUMAN BLOOD ; O-(2,3,4,5,6-PENTAFLUOROBENZYL)OXIME DERIVATIVES ; UNSATURATED ALDEHYDES ; COPEPOD REPRODUCTION ; DIATOMS
WOS研究方向Chemistry
语种英语
WOS记录号WOS:000291986600015
资助机构Chinese Academy of Sciences [KZCX2-YW-Q07-04]; National Science and Technology Support Program [2011BAC02B04]; CAS/SAFEA
公开日期2011-07-22
源URL[http://ir.yic.ac.cn/handle/133337/4844]  
专题烟台海岸带研究所_山东省海岸带环境工程技术研究中心
烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室
中国科学院烟台海岸带研究所
作者单位1.Chinese Acad Sci, Key Lab Coastal Zone Environm Proc, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai 264003, Shandong, Peoples R China
2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Shandong, Peoples R China
3.Qingdao Technol Univ, Inst Environm & Municipal Engn, Key Lab Environm Engn Shandong Prov, Qingdao, Peoples R China
推荐引用方式
GB/T 7714		 Ma, Jiping,Xiao, Ronghui,Li, Jinhua,et al. Headspace solid-phase microextraction with on-fiber derivatization for the determination of aldehydes in algae by gas chromatography-mass spectrometry[J]. JOURNAL OF SEPARATION SCIENCE,2011,34(12):1477-1483.
APA Ma, Jiping.,Xiao, Ronghui.,Li, Jinhua.,Li, Jie.,Shi, Benzhang.,...&Chen, Lingxin.(2011).Headspace solid-phase microextraction with on-fiber derivatization for the determination of aldehydes in algae by gas chromatography-mass spectrometry.JOURNAL OF SEPARATION SCIENCE,34(12),1477-1483.
MLA Ma, Jiping,et al."Headspace solid-phase microextraction with on-fiber derivatization for the determination of aldehydes in algae by gas chromatography-mass spectrometry".JOURNAL OF SEPARATION SCIENCE 34.12(2011):1477-1483.

入库方式： OAI收割

来源：烟台海岸带研究所

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