Cloning and characterization of allograft inflammatory factor-1 (AIF-1) from manila clam Venerupis philippinarum
文献类型：期刊论文
| 作者 | Zhang, Lei1,2,3; Zhao, Jianmin2 ; Li, Chenghua1; Su, Xiurong1; Chen, Aiqin2; Li, Taiwu1; Qin, Song2
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| 刊名 | FISH & SHELLFISH IMMUNOLOGY
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| 出版日期 | 2011 |
| 卷号 | 30期号:1页码:148-153 |
| 关键词 | Allograft Inflammatory Factor-1 Venerupis Philippinarum Immune Response Bacteria Challenge |
| ISSN号 | 1050-4648 |
| 产权排序 | [Zhang, Lei; Li, Chenghua; Su, Xiurong; Li, Taiwu] Ningbo Univ, Fac Life Sci & Biotechnol, Ningbo 315211, Zhejiang, Peoples R China; [Zhang, Lei; Zhao, Jianmin; Chen, Aiqin; Qin, Song] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China; [Zhang, Lei] Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 通讯作者 | Li, CH, Ningbo Univ, Fac Life Sci & Biotechnol, Ningbo 315211, Zhejiang, Peoples R China.chli@yic.ac.cn |
| 文献子类 | Article |
| 英文摘要 | Allograft inflammatory factor-1 (AIF-1) is a 17 kDa interferon-gamma-inducible Ca2+-binding EF-hand protein that plays a significant role not only in different host responses to inflammatory stimuli, but in the whole host immune defense reaction. In the present study, the full-length cDNA of AIF-1 was identified from manila clam Venerupis philippinarum (denoted as VpAIF-1) by cDNA library and RACE approaches. The cDNA of vpAIF-1 consisted of a 5-terminal untranslated region (UTR) of 153 bp, a 3'UTR of 219 bp with a poly (A) tail, and an open reading frame (ORF) of 516 bp encoding a polypeptide of 171 amino acids with the putative molecular mass of 17 kDa. The deduced amino acid of VpAIF-1 shared two EF hand Ca2+-binding motifs like other AIF-1s. Phylogenetic analysis further indicated that VpAIF-1 had higher evolutional conservation to invertebrate than vertebrate counterparts and should be a new member of the AIF-1 protein family. Spatial expression analysis indicated that mRNA transcript of VpAIF-1 was found to be most abundantly expressed in the tissues of haemocytes, gills and hepatopancreas, weakly expressed in the tissues of mantle, muscle, and foot. After challenged by Vibrio anguillarum, the mRNA level of VpAIF-1 in overall haemocytes population was recorded by quantitative real-time RT-PcR. vpAIF-1 mRNA was down-regulated in the first 12 h post-infection. Then, the expression level increased to the peak at 72 h and recovered to the 48 h-level at 96 h. All these results indicated that VpAIF-1 was involved in the immune response against microbe infection and might be contributed to the clearance of bacterial pathogens. (C) 2010 Elsevier Ltd. All rights reserved.; Allograft inflammatory factor-1 (AIF-1) is a 17 kDa interferon-gamma-inducible Ca(2+)-binding EF-hand protein that plays a significant role not only in different host responses to inflammatory stimuli, but in the whole host immune defense reaction. In the present study, the full-length cDNA of AIF-1 was identified from manila clam Venerupis philippinarum (denoted as VpAIF-1) by cDNA library and RACE approaches. The cDNA of vpAIF-1 consisted of a 5-terminal untranslated region (UTR) of 153 bp, a 3'UTR of 219 bp with a poly (A) tail, and an open reading frame (ORF) of 516 bp encoding a polypeptide of 171 amino acids with the putative molecular mass of 17 kDa. The deduced amino acid of VpAIF-1 shared two EF hand Ca(2+)-binding motifs like other AIF-1s. Phylogenetic analysis further indicated that VpAIF-1 had higher evolutional conservation to invertebrate than vertebrate counterparts and should be a new member of the AIF-1 protein family. Spatial expression analysis indicated that mRNA transcript of VpAIF-1 was found to be most abundantly expressed in the tissues of haemocytes, gills and hepatopancreas, weakly expressed in the tissues of mantle, muscle, and foot. After challenged by Vibrio anguillarum, the mRNA level of VpAIF-1 in overall haemocytes population was recorded by quantitative real-time RT-PcR. vpAIF-1 mRNA was down-regulated in the first 12 h post-infection. Then, the expression level increased to the peak at 72 h and recovered to the 48 h-level at 96 h. All these results indicated that VpAIF-1 was involved in the immune response against microbe infection and might be contributed to the clearance of bacterial pathogens. (C) 2010 Elsevier Ltd. All rights reserved. |
| 学科主题 | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| WOS关键词 | MARINE-INVERTEBRATES ; EXPRESSION ; CYTOKINE ; REJECTION ; IMMUNITY ; HEARTS ; GENE |
| WOS研究方向 | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000286905800019 |
| 公开日期 | 2011-07-22 |
| 源URL | [http://ir.yic.ac.cn/handle/133337/4888]  |
| 专题 | 烟台海岸带研究所_污染过程与控制实验室 烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室 |
| 作者单位 | 1.Ningbo Univ, Fac Life Sci & Biotechnol, Ningbo 315211, Zhejiang, Peoples R China 2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Lei,Zhao, Jianmin,Li, Chenghua,et al. Cloning and characterization of allograft inflammatory factor-1 (AIF-1) from manila clam Venerupis philippinarum[J]. FISH & SHELLFISH IMMUNOLOGY,2011,30(1):148-153. |
| APA | Zhang, Lei.,Zhao, Jianmin.,Li, Chenghua.,Su, Xiurong.,Chen, Aiqin.,...&Qin, Song.(2011).Cloning and characterization of allograft inflammatory factor-1 (AIF-1) from manila clam Venerupis philippinarum.FISH & SHELLFISH IMMUNOLOGY,30(1),148-153. |
| MLA | Zhang, Lei,et al."Cloning and characterization of allograft inflammatory factor-1 (AIF-1) from manila clam Venerupis philippinarum".FISH & SHELLFISH IMMUNOLOGY 30.1(2011):148-153. |
入库方式： OAI收割
来源：烟台海岸带研究所
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