Polymeric Membrane Ion-Selective Electrode for Butyrylcholinesterase Based on Controlled Release of Substrate
文献类型：期刊论文
| 作者 | Ding, Jiawang1,2,3 ; Qin, Wei1,4
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| 刊名 | ELECTROANALYSIS
 |
| 出版日期 | 2009-09-01 |
| 卷号 | 21期号:17-18页码:2030-2035 |
| 关键词 | Polymer Membrane Electrodes Controlled-release Butyrylcholinesterase Pesticides Biosensors Membranes |
| 产权排序 | [Ding, Jiawang; Qin, Wei] Chinese Acad Sci, Environm Chem Lab, Yantai Inst Coastal Zone Res Sustainable Dev, Yantai 264003, Peoples R China; [Ding, Jiawang] Chinese Acad Sci, S China Sea Inst Oceanol, Guangzhou 510301, Guangdong, Peoples R China; [Ding, Jiawang] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China; [Qin, Wei] Yantai Univ, Sch Oceanol, Yantai 264005, Peoples R China |
| 通讯作者 | Qin, W, Chinese Acad Sci, Environm Chem Lab, Yantai Inst Coastal Zone Res Sustainable Dev, Yantai 264003, Peoples R China |
| 文献子类 | Article |
| 英文摘要 | A butyrylcholine selective polymeric membrane electrode using o-beta-cyclodextrin as ionophore is described as a reagent controlled-release system for reagentless detection of butyrylcholinesterase. Butyrylcholine released across the membrane from inner filling solution of the ion selective electrode is consumed by reaction of the enzyme in sample solution and the concentration of butyrylcholine at the membrane surface can be sensed potentiometrically. The electrode with 0.01 M butyrylcholine in the inner solution yields a potential that varies linearly with butyrylcholinesterase concentration over the range of 0.0075-0.15 U mL(-1) in 0.02 M of pH 7.4 sodium phosphate buffer solution. This approach can also be used to analyze butyrylcholinesterase inhibitors such as organophosphate pesticides. The inhibition percentage of parathion is proportional to its concentration in the range of 0.05-0.5 ng mL(-1) with a detection limit of 0.03 ng mL(-1).; A butyrylcholine selective polymeric membrane electrode using o-beta-cyclodextrin as ionophore is described as a reagent controlled-release system for reagentless detection of butyrylcholinesterase. Butyrylcholine released across the membrane from inner filling solution of the ion selective electrode is consumed by reaction of the enzyme in sample solution and the concentration of butyrylcholine at the membrane surface can be sensed potentiometrically. The electrode with 0.01 M butyrylcholine in the inner solution yields a potential that varies linearly with butyrylcholinesterase concentration over the range of 0.0075-0.15 U mL(-1) in 0.02 M of pH 7.4 sodium phosphate buffer solution. This approach can also be used to analyze butyrylcholinesterase inhibitors such as organophosphate pesticides. The inhibition percentage of parathion is proportional to its concentration in the range of 0.05-0.5 ng mL(-1) with a detection limit of 0.03 ng mL(-1). |
| 学科主题 | Chemistry ; Analytical ; Electrochemistry |
| WOS关键词 | LOWER DETECTION LIMIT ; POTENTIOMETRIC SENSORS ; ELECTROCHEMICAL ASSAY ; AU NANOPARTICLES ; FLUXES ; ACETYLCHOLINESTERASE ; INHIBITION ; TITRATIONS ; MECHANISM ; CHITOSAN |
| WOS研究方向 | Chemistry ; Electrochemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000270399100020 |
| 源URL | [http://ir.yic.ac.cn/handle/133337/4939]  |
| 专题 | 烟台海岸带研究所_近岸生态与环境实验室 |
| 作者单位 | 1.Chinese Acad Sci, Environm Chem Lab, Yantai Inst Coastal Zone Res Sustainable Dev, Yantai 264003, Peoples R China 2.Chinese Acad Sci, S China Sea Inst Oceanol, Guangzhou 510301, Guangdong, Peoples R China 3.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China 4.Yantai Univ, Sch Oceanol, Yantai 264005, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ding, Jiawang,Qin, Wei. Polymeric Membrane Ion-Selective Electrode for Butyrylcholinesterase Based on Controlled Release of Substrate[J]. ELECTROANALYSIS,2009,21(17-18):2030-2035. |
| APA | Ding, Jiawang,&Qin, Wei.(2009).Polymeric Membrane Ion-Selective Electrode for Butyrylcholinesterase Based on Controlled Release of Substrate.ELECTROANALYSIS,21(17-18),2030-2035. |
| MLA | Ding, Jiawang,et al."Polymeric Membrane Ion-Selective Electrode for Butyrylcholinesterase Based on Controlled Release of Substrate".ELECTROANALYSIS 21.17-18(2009):2030-2035. |
入库方式： OAI收割
来源：烟台海岸带研究所
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