Metabolomic Study on the Halophyte Suaeda salsa in the Yellow River Delta
文献类型:期刊论文
作者 | Liu, Xiaoli1,2; Zhang, Linbao1,2; You, Liping1,2; Wu, Huifeng1![]() ![]() ![]() ![]() ![]() ![]() |
刊名 | CLEAN-SOIL AIR WATER
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出版日期 | 2011-08-01 |
卷号 | 39期号:8页码:720-727 |
关键词 | Metabolite Extraction Metabolomics Nmr Suaeda Salsa |
ISSN号 | 1863-0650 |
产权排序 | [Liu, XL; Zhang, LB; You, LP; Wu, HF; Zhao, JM; Cong, M; Li, F; Wang, Q; Li, LZ; Li, CH; Han, GX; Wang, GM; Xia, CH; Yu, JB] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Shandong Prov Key Lab Coastal Zone Environm Proc, Key Lab Coastal Zone Environm Proc,CAS, Yantai 264003, Peoples R China; [Liu, XL; Zhang, LB; You, LP; Zhao, JM; Cong, M; Li, F; Wang, Q; Li, LZ; Li, CH; Han, GX; Wang, GM; Xia, CH; Yu, JB] Chinese Acad Sci, Grad Sch, Beijing, Peoples R China |
通讯作者 | Wu, HF, Chinese Acad Sci, Yantai Inst Coastal Zone Res, Shandong Prov Key Lab Coastal Zone Environm Proc, Key Lab Coastal Zone Environm Proc,CAS, Yantai 264003, Peoples R China. hfwu@yic.ac.cn |
文献子类 | Article |
英文摘要 | Plant metabolomics has been well established and applied across multiple fields including medicine, biotechnology, and environmental sciences in the post-genomic era. The Chenopodiaceae C(3) halophyte Suaeda salsa is the most important plant species in the vegetation of saline soil and even intertidal zone in the Yellow River Delta, which is economically consumed as food, widely used as a bioindicator of environmental stresses (salinity, drought, and pollution) and typically applied for the phyto-remediation of degraded wetland. However, no global studies have been focused on the metabolic profile of this halophyte which is widely applied in environment related research areas. In metabolomics, the first crucial step is the preparation of plant samples. In this work, several strategies of metabolite extraction from this C(3) halophytes S. salsa were evaluated and the metabolic profile was characterized by NMRbased metabolomics. Multiple replicates of plant tissues (approx. 250 mg whole fresh weight of leaves and stems) were homogenized by a high throughput automated Precellys 24 bead-based homogenizer and then extracted using the following solvent systems of varying polarities:methanol, water, methanol/water, methanol/chloroform/water, and acetonitrile. The hydrophilic metabolites were analyzed using one-dimensional proton NMR spectroscopy, and the subsequent NMR spectra were evaluated by principal components analysis (PCA). Each extraction protocol revealed unique metabolic profile from S. salsa. Overall, the quality of each extraction protocol was assessed based on the yield, reproducibility, ease, and speed, and we concluded that the solvent of methanol/water was preferable for the metabolite extraction due to its highest reproducibility, ease, and speed, and considerably high yield. Validation of methanol/ water (1:1) solvent also showed a high degree of reproducibility with technical variation being considerably lower than biological variation in S. salsa samples. This demonstrated the quality and robustness of the methanol/water extraction method for NMRbased metabolomics studies.; Plant metabolomics has been well established and applied across multiple fields including medicine, biotechnology, and environmental sciences in the post-genomic era. The Chenopodiaceae C-3 halophyte Suaeda salsa is the most important plant species in the vegetation of saline soil and even intertidal zone in the Yellow River Delta, which is economically consumed as food, widely used as a bioindicator of environmental stresses (salinity, drought, and pollution) and typically applied for the phyto-remediation of degraded wetland. However, no global studies have been focused on the metabolic profile of this halophyte which is widely applied in environment related research areas. In metabolomics, the first crucial step is the preparation of plant samples. In this work, several strategies of metabolite extraction from this C-3 halophytes S. salsa were evaluated and the metabolic profile was characterized by NMRbased metabolomics. Multiple replicates of plant tissues (approx. 250 mg whole fresh weight of leaves and stems) were homogenized by a high throughput automated Precellys 24 bead-based homogenizer and then extracted using the following solvent systems of varying polarities:methanol, water, methanol/water, methanol/chloroform/water, and acetonitrile. The hydrophilic metabolites were analyzed using one-dimensional proton NMR spectroscopy, and the subsequent NMR spectra were evaluated by principal components analysis (PCA). Each extraction protocol revealed unique metabolic profile from S. salsa. Overall, the quality of each extraction protocol was assessed based on the yield, reproducibility, ease, and speed, and we concluded that the solvent of methanol/water was preferable for the metabolite extraction due to its highest reproducibility, ease, and speed, and considerably high yield. Validation of methanol/ water (1:1) solvent also showed a high degree of reproducibility with technical variation being considerably lower than biological variation in S. salsa samples. This demonstrated the quality and robustness of the methanol/water extraction method for NMRbased metabolomics studies. |
学科主题 | Environmental Sciences & Ecology ; Marine & Freshwater Biology ; Water Resources |
WOS关键词 | V-H+-ATPASE ; BETAINE-HOMOCYSTEINE METHYLTRANSFERASE ; PLANT METABOLOMICS ; SAMPLE-PREPARATION ; NMR-SPECTROSCOPY ; OSMOTIC-STRESS ; LEAVES ; EXTRACTION ; LEAF ; ACCUMULATION |
WOS研究方向 | Environmental Sciences & Ecology ; Marine & Freshwater Biology ; Water Resources |
语种 | 英语 |
WOS记录号 | WOS:000294720700002 |
资助机构 | National Science & Technology Pillar Program in "12th Five Year'' Period[2011BAC02B01]; Chinese Academy of Sciences[KZCX2-YW-223, KZCX2-YW-225]; Technology Development Program Projects of Shandong Province[2008GG20005006, 2008GG3NS0700]; SDSFC[ZR2009CZ008]; CAS/SAFEA; Shandong Provincial Key Laboratory of Ecology and Environment in the Yellow River Delta[2009KFJJ03] |
公开日期 | 2011-11-11 |
源URL | [http://ir.yic.ac.cn/handle/133337/5122] ![]() |
专题 | 烟台海岸带研究所_近岸生态与环境实验室 烟台海岸带研究所_滨海湿地实验室 烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室 中国科学院烟台海岸带研究所 |
作者单位 | 1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Shandong Prov Key Lab Coastal Zone Environm Proc, Key Lab Coastal Zone Environm Proc,CAS, Yantai 264003, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China |
推荐引用方式 GB/T 7714 | Liu, Xiaoli,Zhang, Linbao,You, Liping,et al. Metabolomic Study on the Halophyte Suaeda salsa in the Yellow River Delta[J]. CLEAN-SOIL AIR WATER,2011,39(8):720-727. |
APA | Liu, Xiaoli.,Zhang, Linbao.,You, Liping.,Wu, Huifeng.,Zhao, Jianmin.,...&Yu, Junbao.(2011).Metabolomic Study on the Halophyte Suaeda salsa in the Yellow River Delta.CLEAN-SOIL AIR WATER,39(8),720-727. |
MLA | Liu, Xiaoli,et al."Metabolomic Study on the Halophyte Suaeda salsa in the Yellow River Delta".CLEAN-SOIL AIR WATER 39.8(2011):720-727. |
入库方式: OAI收割
来源:烟台海岸带研究所
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