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  4. 山东省海岸带环境工程技术研究中心
ANALYSIS OF URINARY PORPHYRINS BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY-ELECTROSPRAY IONIZATION MASS SPECTROMETRY

文献类型：期刊论文

作者Li, Jinhua1,2; Cai, Zongwei2; Xu, Shoufang1,4; Liao, Chunyang1; Song, Xingliang3; Chen, Lingxin1
刊名JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
出版日期2011
卷号34期号:15页码:1578-1593
关键词Electrospray Ionization High Performance Liquid Chromatography Human Urine Mass Spectrometry Porphyrins Solid-phase Extraction
ISSN号1082-6076
产权排序[Li, Jinhua; Xu, Shoufang; Liao, Chunyang; Chen, Lingxin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China; [Li, Jinhua; Cai, Zongwei] Hong Kong Baptist Univ, Dept Chem, Hong Kong, Hong Kong, Peoples R China; [Song, Xingliang] Linyi Univ, Dept Chem, Linyi, Peoples R China; [Xu, Shoufang] Chinese Acad Sci, Grad Univ, Beijing, Peoples R China
通讯作者Chen, LX (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China
文献子类Article
英文摘要A high-performance liquid chromatography coupled with electrospray ionization mass spectrometry (HPLC-ESI-MS) method was developed for the simultaneous separation and detection of six porphyrins, namely uroporphyrin (UP), heptacarboxylic acid porphyrin (HEPTAP), hexacarboxylic acid porphyrin (HEXAP), pentacarboxylic acid porphyrin (PENTAP), coproporphyrin (CP), and mesoporphyrin (MP), in human urine samples. The recoveries of the porphyrins in urine ranged from 84 to 108%. The intra-and inter-day precisions at spiked porphyrin concentrations were 4.0-9.7% and 5.5-15%, respectively. The limits of detection (LOD) ranged from 0.2 to 3 nM. The developed method was successfully applied to the determination of endogenous porphyrins in human urine. UP, HEPTAP, and PENTAP were detected at levels of 42.4, 21.3, and 18.0 nM, respectively. CP was detected as the predominant species at 135.6 nM, wheres MP and HEXAP were not detected in the urine samples from healthy females. Significant increases of CP excretion in urine from liver disease patients were observed. This method proved potentially applicable for the routine diagnosis and treatment of porphyrin-related diseases.; A high-performance liquid chromatography coupled with electrospray ionization mass spectrometry (HPLC-ESI-MS) method was developed for the simultaneous separation and detection of six porphyrins, namely uroporphyrin (UP), heptacarboxylic acid porphyrin (HEPTAP), hexacarboxylic acid porphyrin (HEXAP), pentacarboxylic acid porphyrin (PENTAP), coproporphyrin (CP), and mesoporphyrin (MP), in human urine samples. The recoveries of the porphyrins in urine ranged from 84 to 108%. The intra-and inter-day precisions at spiked porphyrin concentrations were 4.0-9.7% and 5.5-15%, respectively. The limits of detection (LOD) ranged from 0.2 to 3 nM. The developed method was successfully applied to the determination of endogenous porphyrins in human urine. UP, HEPTAP, and PENTAP were detected at levels of 42.4, 21.3, and 18.0 nM, respectively. CP was detected as the predominant species at 135.6 nM, wheres MP and HEXAP were not detected in the urine samples from healthy females. Significant increases of CP excretion in urine from liver disease patients were observed. This method proved potentially applicable for the routine diagnosis and treatment of porphyrin-related diseases.
学科主题Biochemistry & Molecular Biology ; Chemistry
URL标识查看原文
WOS关键词CAPILLARY-ELECTROPHORESIS ; ELECTROCHEMICAL DETECTION ; FLUORESCENCE DETECTION ; HPLC METHOD ; SEPARATION ; EXTRACTION ; DIAGNOSIS ; STACKING ; ISOMERS ; CLEANUP
WOS研究方向Biochemistry & Molecular Biology ; Chemistry
语种英语
WOS记录号WOS:000296231000009
资助机构National Natural Science Foundation of China[20975089, 20907039]; Department of Science and Technology of Shandong Province of China[2008GG20005005, 2010GSF10222]; Natural Science Foundation of Shandong Province of China[Y2007B38]; Yantai Research and Development Program[2007323]; Chinese Academy of Sciences
公开日期2012-03-06
源URL[http://ir.yic.ac.cn/handle/133337/5327]  
专题烟台海岸带研究所_山东省海岸带环境工程技术研究中心
烟台海岸带研究所_污染过程与控制实验室
作者单位1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China
2.Hong Kong Baptist Univ, Dept Chem, Hong Kong, Hong Kong, Peoples R China
3.Linyi Univ, Dept Chem, Linyi, Peoples R China
4.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China
推荐引用方式
GB/T 7714		 Li, Jinhua,Cai, Zongwei,Xu, Shoufang,et al. ANALYSIS OF URINARY PORPHYRINS BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY-ELECTROSPRAY IONIZATION MASS SPECTROMETRY[J]. JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,2011,34(15):1578-1593.
APA Li, Jinhua,Cai, Zongwei,Xu, Shoufang,Liao, Chunyang,Song, Xingliang,&Chen, Lingxin.(2011).ANALYSIS OF URINARY PORPHYRINS BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY-ELECTROSPRAY IONIZATION MASS SPECTROMETRY.JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,34(15),1578-1593.
MLA Li, Jinhua,et al."ANALYSIS OF URINARY PORPHYRINS BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY-ELECTROSPRAY IONIZATION MASS SPECTROMETRY".JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES 34.15(2011):1578-1593.

入库方式： OAI收割

来源：烟台海岸带研究所

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