中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
首页 机构 成果 学者
  
  1. CAS IR Grid
  2. 烟台海岸带研究所
  3. 中国科学院烟台海岸带研究所
  4. 中科院海岸带环境过程与生态修复重点实验室
  5. 污染过程与控制实验室
Molecular cloning and characterization of two isoforms of cyclophilin A gene from Venerupis philippinarum

文献类型：期刊论文

作者Chen, Leilei1; Mu, Changkao1; Zhao, Jianmin2; Wang, Chunlin1
刊名FISH & SHELLFISH IMMUNOLOGY
出版日期2011-12-01
卷号31期号:6页码:1218-1223
关键词Venerupis Philippinarum Cyclophilin a Gene Expression
ISSN号1050-4648
产权排序[Chen, Leilei; Mu, Changkao; Wang, Chunlin] Ningbo Univ, Fac Life Sci & Biotechnol, Ningbo 315211, Zhejiang, Peoples R China; [Zhao, Jianmin] Chinese Acad Sci, Key Lab Coastal Zone Environm Proc, Shandong Prov Key Lab Coastal Zone Environm Proc, CAS,Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China
通讯作者Wang, CL (reprint author), Ningbo Univ, Fac Life Sci & Biotechnol, Ningbo 315211, Zhejiang, Peoples R China
文献子类Article
英文摘要Cyclophilin A (CypA) is a ubiquitously distributed intracellular protein belonging to the immunophilin family, which is recognized as the cell receptor for the potent immunosuppressive drug cyclosporine A. In the present study, two isoforms of cyclophilin A gene (named as VpCypA1 and VpCypA2) were isolated and characterized from Venerupis philippinarum by RACE approaches. Both VpCypA1 and VpCypA2 possessed all conserved features critical for the fundamental structure and function of CypA, indicating that the two isoforms of cyclophilin A should be new members of CypA family. The expression of VpCypA2 mRNA in haemocytes was significantly up-regulated and the highest expression level was detected at 96 h post-infection with 7.7-fold increase compared with that in the blank group. On the contrary, the relative expression level of VpCypA1 mRNA was down-regulated rapidly at 6 h post-infection and reached 0.4-fold of the control group. They exhibited different expression profile and identical effect of immune modulation, which might suggest the two VpCypA isoforms exert their function in a manner of synergy. These results provide valuable information for further exploring the roles of cyclophilin A in the immune responses of V. philippinarum. (C) 2011 Elsevier Ltd. All rights reserved.; Cyclophilin A (CypA) is a ubiquitously distributed intracellular protein belonging to the immunophilin family, which is recognized as the cell receptor for the potent immunosuppressive drug cyclosporine A. In the present study, two isoforms of cyclophilin A gene (named as VpCypA1 and VpCypA2) were isolated and characterized from Venerupis philippinarum by RACE approaches. Both VpCypA1 and VpCypA2 possessed all conserved features critical for the fundamental structure and function of CypA, indicating that the two isoforms of cyclophilin A should be new members of CypA family. The expression of VpCypA2 mRNA in haemocytes was significantly up-regulated and the highest expression level was detected at 96 h post-infection with 7.7-fold increase compared with that in the blank group. On the contrary, the relative expression level of VpCypA1 mRNA was down-regulated rapidly at 6 h post-infection and reached 0.4-fold of the control group. They exhibited different expression profile and identical effect of immune modulation, which might suggest the two VpCypA isoforms exert their function in a manner of synergy. These results provide valuable information for further exploring the roles of cyclophilin A in the immune responses of V. philippinarum. (C) 2011 Elsevier Ltd. All rights reserved.
学科主题Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
URL标识查看原文
WOS关键词CIS-TRANS-ISOMERASE ; BINDING-PROTEIN CYCLOPHILIN ; OXIDATIVE STRESS ; ZHIKONG SCALLOP ; CHLAMYS-FARRERI ; CYCLOSPORINE-A ; EXPRESSION ; FAMILY ; IMMUNOPHILINS ; MECHANISM
WOS研究方向Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
语种英语
WOS记录号WOS:000298569700063
资助机构Innovation Research Team in University[IRT0734]; Ministry of Education of China[20103305110003, 20103305120002]; Nature Fund of Ningbo[2010A610004]; Ningbo University
公开日期2012-03-06
源URL[http://ir.yic.ac.cn/handle/133337/5381]  
专题烟台海岸带研究所_污染过程与控制实验室
烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室
作者单位1.Ningbo Univ, Fac Life Sci & Biotechnol, Ningbo 315211, Zhejiang, Peoples R China
2.Chinese Acad Sci, Key Lab Coastal Zone Environm Proc, Shandong Prov Key Lab Coastal Zone Environm Proc, CAS,Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China
推荐引用方式
GB/T 7714		 Chen, Leilei,Mu, Changkao,Zhao, Jianmin,et al. Molecular cloning and characterization of two isoforms of cyclophilin A gene from Venerupis philippinarum[J]. FISH & SHELLFISH IMMUNOLOGY,2011,31(6):1218-1223.
APA Chen, Leilei,Mu, Changkao,Zhao, Jianmin,&Wang, Chunlin.(2011).Molecular cloning and characterization of two isoforms of cyclophilin A gene from Venerupis philippinarum.FISH & SHELLFISH IMMUNOLOGY,31(6),1218-1223.
MLA Chen, Leilei,et al."Molecular cloning and characterization of two isoforms of cyclophilin A gene from Venerupis philippinarum".FISH & SHELLFISH IMMUNOLOGY 31.6(2011):1218-1223.

入库方式： OAI收割

来源：烟台海岸带研究所

浏览0
下载0
收藏0
其他版本

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。