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Transcriptional regulation of selenium-dependent glutathione peroxidase from Venerupis philippinarum in response to pathogen and contaminants challenge

文献类型：期刊论文

作者Zhang, Linbao1,2; Liu, Xiaoli1,2; Chen, Leilei3; You, Liping1,2; Pei, Dong4; Cong, Ming1; Zhao, Jianmin1; Li, Chenghua1; Liu, Dongyan1; Yu, Junbao1
刊名FISH & SHELLFISH IMMUNOLOGY
出版日期2011-12-01
卷号31期号:6页码:831-837
关键词Venerupis Philippinarum Selenium-dependent Glutathione Peroxidase Bacterial Challenge Heavy Metals Benzo[a]Pyrene
ISSN号1050-4648
产权排序[Zhang, Linbao; Liu, Xiaoli; You, Liping; Cong, Ming; Zhao, Jianmin; Li, Chenghua; Liu, Dongyan; Yu, Junbao; Wu, Huifeng] Chinese Acad Sci, Yantai Inst Coastal Zone Res, CAS Shandong Prov Key Lab Coastal Zone Environm P, Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China; [Zhang, Linbao; Liu, Xiaoli; You, Liping] Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China; [Chen, Leilei] Ningbo Univ, Fac Life Sci & Biotechnol, Ningbo 315211, Peoples R China; [Pei, Dong] China Agr Univ Yantai, Yantai 264670, Peoples R China
通讯作者Zhao, JM (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, CAS Shandong Prov Key Lab Coastal Zone Environm P, Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China
文献子类Article
英文摘要Glutathione peroxidases (GPx) are key enzymes in the antioxidant systems of living organisms by catalyzing the reduction of peroxides to non-reactive products. In the present study, the full-length cDNA encoding a selenium-dependent GPx was identified from Venerupis philippinarum (designated as VpSe-GPx), and the spatial and temporal expression patterns post-Vibrio anguillarum, heavy metals and benzo[a]pyrene (B[a]P) challenge were also investigated. VpSe-GPx possessed all the conserved features critical for the fundamental structure and function of glutathione peroxidase. The VpSe-GPx mRNA was found to be most abundantly expressed in hepatopancreas. Vibrio challenge could significantly up-regulate the mRNA expression of VpSe-GPx, and the highest expression level was detected at 24 h post-infection with 6.5-fold increase compared with that in the control group. For heavy metals exposure, the expression of VpSe-GPx was significantly induced by 20, 40 mu g L(-1) Cd and 10, 20 mu g L(-1) Cu but depressed by 10 mu g L(-1) Cd and 40 mu g L(-1) Cu. With regards to B[a]P exposure, the expression of VpSe-GPx mRNA was significantly induced at 48 and 96 h post challenge. All these results suggested that VpSe-GPx was potentially involved in mediating the immune response and antioxidant defense in V. Philippinarum. (C) 2011 Elsevier Ltd. All rights reserved.; Glutathione peroxidases (GPx) are key enzymes in the antioxidant systems of living organisms by catalyzing the reduction of peroxides to non-reactive products. In the present study, the full-length cDNA encoding a selenium-dependent GPx was identified from Venerupis philippinarum (designated as VpSe-GPx), and the spatial and temporal expression patterns post-Vibrio anguillarum, heavy metals and benzo[a]pyrene (B[a]P) challenge were also investigated. VpSe-GPx possessed all the conserved features critical for the fundamental structure and function of glutathione peroxidase. The VpSe-GPx mRNA was found to be most abundantly expressed in hepatopancreas. Vibrio challenge could significantly up-regulate the mRNA expression of VpSe-GPx, and the highest expression level was detected at 24 h post-infection with 6.5-fold increase compared with that in the control group. For heavy metals exposure, the expression of VpSe-GPx was significantly induced by 20, 40 mu g L(-1) Cd and 10, 20 mu g L(-1) Cu but depressed by 10 mu g L(-1) Cd and 40 mu g L(-1) Cu. With regards to B[a]P exposure, the expression of VpSe-GPx mRNA was significantly induced at 48 and 96 h post challenge. All these results suggested that VpSe-GPx was potentially involved in mediating the immune response and antioxidant defense in V. Philippinarum. (C) 2011 Elsevier Ltd. All rights reserved.
学科主题Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
URL标识查看原文
WOS关键词CLAM RUDITAPES-DECUSSATUS ; OXIDATIVE STRESS ; CRASSOSTREA-GIGAS ; REACTIVE OXYGEN ; FREE-RADICALS ; DNA-DAMAGE ; EXPRESSION ; IDENTIFICATION ; ANTIOXIDANTS ; INFECTION
WOS研究方向Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
语种英语
WOS记录号WOS:000298569700014
资助机构Chinese Academy of Sciences, Technology Development Program Projects of Shandong Province[2008GG20005006, 2008GG3NS0700]; SDSFC[ZR2009CZ008]; CAS/SAFEA
公开日期2012-03-06
源URL[http://ir.yic.ac.cn/handle/133337/5397]  
专题烟台海岸带研究所_污染过程与控制实验室
烟台海岸带研究所_近岸生态与环境实验室
烟台海岸带研究所_滨海湿地实验室
烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室
作者单位1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, CAS Shandong Prov Key Lab Coastal Zone Environm P, Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
3.Ningbo Univ, Fac Life Sci & Biotechnol, Ningbo 315211, Peoples R China
4.China Agr Univ Yantai, Yantai 264670, Peoples R China
推荐引用方式
GB/T 7714		 Zhang, Linbao,Liu, Xiaoli,Chen, Leilei,et al. Transcriptional regulation of selenium-dependent glutathione peroxidase from Venerupis philippinarum in response to pathogen and contaminants challenge[J]. FISH & SHELLFISH IMMUNOLOGY,2011,31(6):831-837.
APA Zhang, Linbao.,Liu, Xiaoli.,Chen, Leilei.,You, Liping.,Pei, Dong.,...&Wu, Huifeng.(2011).Transcriptional regulation of selenium-dependent glutathione peroxidase from Venerupis philippinarum in response to pathogen and contaminants challenge.FISH & SHELLFISH IMMUNOLOGY,31(6),831-837.
MLA Zhang, Linbao,et al."Transcriptional regulation of selenium-dependent glutathione peroxidase from Venerupis philippinarum in response to pathogen and contaminants challenge".FISH & SHELLFISH IMMUNOLOGY 31.6(2011):831-837.

入库方式： OAI收割

来源：烟台海岸带研究所

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