中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
首页 机构 成果 学者
  
  1. CAS IR Grid
  2. 烟台海岸带研究所
  3. 中国科学院烟台海岸带研究所
  4. 中科院海岸带环境过程与生态修复重点实验室
  5. 污染过程与控制实验室
Expression profiles of seven glutathione S-transferase (GST) genes from Venerupis philippinarum exposed to heavy metals and benzo[a]pyrene

文献类型：期刊论文

作者Zhang, Linbao1,3; Qiu, Lihua2; Wu, Huifeng1; Liu, Xiaoli1,3; You, Liping1,3; Pei, Dong4; Chen, Leilei1; Wang, Qing1; Zhao, Jianmin1
刊名COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
出版日期2012-04-01
卷号155期号:3页码:517-527
关键词Venerupis Philippinarum Glutathione S-transferase Heavy Metals Benzo[a]Pyrene Biomarker
ISSN号1532-0456
产权排序[Zhang, Linbao; Wu, Huifeng; Liu, Xiaoli; You, Liping; Chen, Leilei; Wang, Qing; Zhao, Jianmin] Chinese Acad Sci, Shandong Prov Key Lab Coastal Zone Environm Proc, Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res YIC,YICCAS, Yantai 264003, Shandong, Peoples R China; [Qiu, Lihua] Chinese Acad Fishery Sci, S China Sea Fisheries Res Inst, Guangzhou 510300, Guangdong, Peoples R China; [Zhang, Linbao; Liu, Xiaoli; You, Liping] Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China; [Pei, Dong] China Agr Univ Yantai, Yantai 264670, Shandong, Peoples R China
通讯作者Wu, HF (reprint author), Chinese Acad Sci, Shandong Prov Key Lab Coastal Zone Environm Proc, Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res YIC,YICCAS, Yantai 264003, Shandong, Peoples R China. hfwu@yic.ac.cn ; jmzhao@yic.ac.cn
文献子类Article
英文摘要Glutathione S-transferases (GSTs) are phase II enzymes that facilitate the detoxification of xenobiotics, and also play important roles in antioxidant defense. In this study, we reported the cloning and molecular characteristics of seven genes of the GST family (VpGSTS1, VpGSTS2, VpGS7S3, VpGSTO, VpGSTMi, VpGSTM and VpGSTR) from Venerupis philippinarum together with mRNA tissue distribution patterns and temporal expression profiles in response to cadmium, copper and benzo[a]pyrene (B[a]P) exposures. The deduced amino acid sequences of VpGSTs showed high similarities to counterparts of other species that clustered into the same clades in the phylogenetic analysis. At basal levels of tissue expression, most VpGSTs were highly expressed in hepatopancreas compared with other tissues. All VpGSTs showed differential response profiles depending on the concentrations of various toxicants and exposure times. More notably, the expressions of VpGSTS2 and VpGSTS3 transcripts were significantly up-regulated in hepatopancreas from Cu and B[a]P-exposed animals, indicating that these two sigma VpGSTs were highly sensitive to Cu and B[a]P exposure. However, the expressions of VpGSTM and VpGSTR were significantly induced by Cu or B[a]P exposure, respectively. These findings suggested the role of VpGSTS2, VpGSTS3, VpGSTM and VpGSTR in defense against oxidative stress and highlighted their potential as biomarkers to Cu or B[a]P exposure.; Glutathione S-transferases (GSTs) are phase II enzymes that facilitate the detoxification of xenobiotics, and also play important roles in antioxidant defense. In this study, we reported the cloning and molecular characteristics of seven genes of the GST family (VpGSTS1, VpGSTS2, VpGS7S3, VpGSTO, VpGSTMi, VpGSTM and VpGSTR) from Venerupis philippinarum together with mRNA tissue distribution patterns and temporal expression profiles in response to cadmium, copper and benzo[a]pyrene (B[a]P) exposures. The deduced amino acid sequences of VpGSTs showed high similarities to counterparts of other species that clustered into the same clades in the phylogenetic analysis. At basal levels of tissue expression, most VpGSTs were highly expressed in hepatopancreas compared with other tissues. All VpGSTs showed differential response profiles depending on the concentrations of various toxicants and exposure times. More notably, the expressions of VpGSTS2 and VpGSTS3 transcripts were significantly up-regulated in hepatopancreas from Cu and B[a]P-exposed animals, indicating that these two sigma VpGSTs were highly sensitive to Cu and B[a]P exposure. However, the expressions of VpGSTM and VpGSTR were significantly induced by Cu or B[a]P exposure, respectively. These findings suggested the role of VpGSTS2, VpGSTS3, VpGSTM and VpGSTR in defense against oxidative stress and highlighted their potential as biomarkers to Cu or B[a]P exposure. (C) 2012 Elsevier Inc. All rights reserved.
学科主题Biochemistry & Molecular Biology ; Endocrinology & Metabolism ; Toxicology ; Zoology
URL标识查看原文
WOS关键词FISH RIVULUS-MARMORATUS ; HALIOTIS-DISCUS-DISCUS ; MOLECULAR-CLONING ; OMEGA-CLASS ; MYTILUS-GALLOPROVINCIALIS ; AROMATIC-HYDROCARBONS ; METABOLIC-ACTIVATION ; DNA-DAMAGE ; MU-CLASS ; LIVER
WOS研究方向Biochemistry & Molecular Biology ; Endocrinology & Metabolism ; Toxicology ; Zoology
语种英语
WOS记录号WOS:000301312600011
资助机构Chinese Academy of Sciences; Key Laboratory of Fisheries Genetic Resources & Aquaculture, Chinese Academy of Fisheries Sciences; Key Laboratory of Marine Spill Oil Identification and Damage Assessment Technology[201115]; State Oceanic Administration
公开日期2012-06-13
源URL[http://ir.yic.ac.cn/handle/133337/5606]  
专题烟台海岸带研究所_污染过程与控制实验室
烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室
海岸带生物资源高效利用研究与发展中心
作者单位1.Chinese Acad Sci, Shandong Prov Key Lab Coastal Zone Environm Proc, Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res YIC,YICCAS, Yantai 264003, Shandong, Peoples R China
2.Chinese Acad Fishery Sci, S China Sea Fisheries Res Inst, Guangzhou 510300, Guangdong, Peoples R China
3.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
4.China Agr Univ Yantai, Yantai 264670, Shandong, Peoples R China
推荐引用方式
GB/T 7714		 Zhang, Linbao,Qiu, Lihua,Wu, Huifeng,et al. Expression profiles of seven glutathione S-transferase (GST) genes from Venerupis philippinarum exposed to heavy metals and benzo[a]pyrene[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY,2012,155(3):517-527.
APA Zhang, Linbao.,Qiu, Lihua.,Wu, Huifeng.,Liu, Xiaoli.,You, Liping.,...&Zhao, Jianmin.(2012).Expression profiles of seven glutathione S-transferase (GST) genes from Venerupis philippinarum exposed to heavy metals and benzo[a]pyrene.COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY,155(3),517-527.
MLA Zhang, Linbao,et al."Expression profiles of seven glutathione S-transferase (GST) genes from Venerupis philippinarum exposed to heavy metals and benzo[a]pyrene".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY 155.3(2012):517-527.

入库方式： OAI收割

来源：烟台海岸带研究所

浏览0
下载0
收藏0
其他版本

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。