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Polymeric Membrane Neutral Phenol-Sensitive Electrodes for Potentiometric G-Quadruplex/Hemin DNAzyme-Based Biosensing

文献类型：期刊论文

作者Wang, Xuewei1,2; Ding, Zhaofeng3; Ren, Qingwei3; Qin, Wei1
刊名ANALYTICAL CHEMISTRY
出版日期2013-02-05
卷号85期号:3页码:1945-1950
关键词Enzyme-immunoassay P-cresol Dna Derivatives Mechanism Oxidation
ISSN号0003-2700
产权排序[Wang, Xuewei; Qin, Wei] Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Shandong Prov Key Lab Coastal Zone Environm Proc, Key Lab Coastal Zone Environm Proc,YICCAS, Yantai 264003, Peoples R China; [Wang, Xuewei] Univ Chinese Acad Sci, Beijing 100049, Peoples R China; [Ding, Zhaofeng; Ren, Qingwei] Yantai Univ, Coll Chem & Chem Engn, Yantai 264003, Peoples R China
通讯作者Qin, W (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Shandong Prov Key Lab Coastal Zone Environm Proc, Key Lab Coastal Zone Environm Proc,YICCAS, Yantai 264003, Peoples R China. wqin@yic.ac.cn
文献子类Article
英文摘要The first potentiometric transducer for G-quadruplex/hemin DNAzyme-based biosensing has been developed by using potential responses of electrically neutral oligomeric phenols on polymeric membrane electrodes. In the presence of G-quadruplex/hemin DNAzyme and H2O2, monomeric phenols (e.g., phenol, methylphenols, and methoxyphenols) can be condensed into oligomeric phenols. Because both substrates and products are nonionic under optimal pH conditions, these reactions are traditionally not considered in designing potentiometric biosensing schemes. However, in this paper, the electrically neutral oligomeric phenols have been found to induce highly sensitive potential responses on quaternary ammonium salt-doped polymeric membrane electrodes owing to their high lipophilicities. In contrast, the potential responses to monomeric phenolic substrates are rather low. Thus, the G-quadruplex/hemin DNAzyme-catalyzed oxidative coupling of monomeric phenols can induce large potential signals, and the catalytic activities of DNAzymes can be probed. A comparison of potential responses induced by peroxidations of 13 monomeric phenols indicates that p-methoxyphenol is the most efficient substrate for potentiometric detection of G-quadruplex/hemin DNAzymes. Finally, two label-free and separation-free potentiometric DNA assay protocols based on the G-quadruplex/hemin DNAzyme have been developed with sensitivities higher than those of colorimetric and fluorometric methods. Coupled with other features such as reliable instrumentation, low cost, ease of miniaturization, and resistance to color and turbid interferences, the proposed polymeric membrane-based potentiometric sensor promises to be a competitive transducer for peroxidase-mimicking DNAzyme-involved biosensing.; The first potentiometric transducer for G-quadruplex/hemin DNAzyme-based biosensing has been developed by using potential responses of electrically neutral oligomeric phenols on polymeric membrane electrodes. In the presence of G-quadruplex/hemin DNAzyme and H2O2, monomeric phenols (e.g., phenol, methylphenols, and methoxyphenols) can be condensed into oligomeric phenols. Because both substrates and products are nonionic under optimal pH conditions, these reactions are traditionally not considered in designing potentiometric biosensing schemes. However, in this paper, the electrically neutral oligomeric phenols have been found to induce highly sensitive potential responses on quaternary ammonium salt-doped polymeric membrane electrodes owing to their high lipophilicities. In contrast, the potential responses to monomeric phenolic substrates are rather low. Thus, the G-quadruplex/hemin DNAzyme-catalyzed oxidative coupling of monomeric phenols can induce large potential signals, and the catalytic activities of DNAzymes can be probed. A comparison of potential responses induced by peroxidations of 13 monomeric phenols indicates that p-methoxyphenol is the most efficient substrate for potentiometric detection of G-quadruplex/hemin DNAzymes. Finally, two label-free and separation-free potentiometric DNA assay protocols based on the G-quadruplex/hemin DNAzyme have been developed with sensitivities higher than those of colorimetric and fluorometric methods. Coupled with other features such as reliable instrumentation, low cost, ease of miniaturization, and resistance to color and turbid interferences, the proposed polymeric membrane-based potentiometric sensor promises to be a competitive transducer for peroxidase-mimicking DNAzyme-involved biosensing.
学科主题Chemistry, Analytical
URL标识查看原文
WOS关键词ENZYME-IMMUNOASSAY ; P-CRESOL ; DNA ; DERIVATIVES ; MECHANISM ; OXIDATION
WOS研究方向Chemistry
语种英语
WOS记录号WOS:000314676100099
资助机构Chinese Academy of Sciences [YZ201161]; National Natural Science Foundation of China [41176081]; Taishan Scholar Program of Shandong Province [TS20081159]
公开日期2013-08-15
源URL[http://ir.yic.ac.cn/handle/133337/6649]  
专题烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室
作者单位1.Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Shandong Prov Key Lab Coastal Zone Environm Proc, Key Lab Coastal Zone Environm Proc,YICCAS, Yantai 264003, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Yantai Univ, Coll Chem & Chem Engn, Yantai 264003, Peoples R China
推荐引用方式
GB/T 7714		 Wang, Xuewei,Ding, Zhaofeng,Ren, Qingwei,et al. Polymeric Membrane Neutral Phenol-Sensitive Electrodes for Potentiometric G-Quadruplex/Hemin DNAzyme-Based Biosensing[J]. ANALYTICAL CHEMISTRY,2013,85(3):1945-1950.
APA Wang, Xuewei,Ding, Zhaofeng,Ren, Qingwei,&Qin, Wei.(2013).Polymeric Membrane Neutral Phenol-Sensitive Electrodes for Potentiometric G-Quadruplex/Hemin DNAzyme-Based Biosensing.ANALYTICAL CHEMISTRY,85(3),1945-1950.
MLA Wang, Xuewei,et al."Polymeric Membrane Neutral Phenol-Sensitive Electrodes for Potentiometric G-Quadruplex/Hemin DNAzyme-Based Biosensing".ANALYTICAL CHEMISTRY 85.3(2013):1945-1950.

入库方式： OAI收割

来源：烟台海岸带研究所

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