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Potentiometric aptasensing based on target-induced conformational switch of a DNA probe using a polymeric membrane silver ion-selective electrode

文献类型：期刊论文

作者Ding, Jiawang1,2,3; Qin, Wei1,2; Zhang, Yan1,2; Wang, Xuewei1,2
刊名BIOSENSORS & BIOELECTRONICS
出版日期2013-07-15
卷号45页码:148-151
关键词Potentiometry Aptasensing Ion-selective Electrode Silver Ions Cytosine
ISSN号0956-5663
产权排序[Ding, Jiawang; Qin, Wei; Zhang, Yan; Wang, Xuewei] Chinese Acad Sci, Key Lab Coastal Zone Environm Processes & Ecol Re, Yantai Inst Coastal Zone Res YIC, Yantai 264003, Shandong, Peoples R China; [Ding, Jiawang; Qin, Wei; Zhang, Yan; Wang, Xuewei] YICCAS, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai 264003, Shandong, Peoples R China; [Ding, Jiawang] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
通讯作者Qin, W (reprint author), Chinese Acad Sci, Key Lab Coastal Zone Environm Processes & Ecol Re, Yantai Inst Coastal Zone Res YIC, Yantai 264003, Shandong, Peoples R China. wqin@yic.ac.cn
文献子类Article
英文摘要In this article, we introduce a general, sensitive, facile, and label-free potentiometric assay based on metal-mediated DNA base pairs. A nucleic acid with one adenosine-5'-triphosphate (ATP) binding sequence (aptamer) in the middle and two cytosine(C)-rich sequences at the lateral portions was employed as a model. A rigid hairpin structure can be formed in the presence of Ag+ ions, in which the C residues of the spatially separated nucleotides are linked by the ions. The strong interaction between Ag+ ions and cytosines forms a stable C-Ag+-C structure, which could reduce the concentration of silver ions released from the polymeric membrane silver ion-selective electrode (ISE) at the sample-membrane interface and decrease the potential response. In the presence of its target, the aptamer (the loop sequence of the probe) binds specifically to the target via reaction incubation. Such target-binding induced aptamer conformational change prevents the formation of C-Ag+-C structure, leaving more silver ions at the sample-membrane interface, which can be detected by the silver ISE. ATP can be quantified in the range of 0.5-3.0 mu M with a detection limit of 0.37 mu M. The relative standard deviation for 5 mu M ATP is 5.5%. For the proposed method, the combination of using ion fluxes of silver ions as modulating reagents and as signal reporters greatly simplifies the detection procedures. In addition, by changing the binding sequence in the middle of the probe, the present detection method will be able to explore new applications of ISE for the detection of a large variety of targets. (C) 2013 Elsevier B.V. All rights reserved.; In this article, we introduce a general, sensitive, facile, and label-free potentiometric assay based on metal-mediated DNA base pairs. A nucleic acid with one adenosine-5'-triphosphate (ATP) binding sequence (aptamer) in the middle and two cytosine(C)-rich sequences at the lateral portions was employed as a model. A rigid hairpin structure can be formed in the presence of Ag+ ions, in which the C residues of the spatially separated nucleotides are linked by the ions. The strong interaction between Ag+ ions and cytosines forms a stable C-Ag+-C structure, which could reduce the concentration of silver ions released from the polymeric membrane silver ion-selective electrode (ISE) at the sample-membrane interface and decrease the potential response. In the presence of its target, the aptamer (the loop sequence of the probe) binds specifically to the target via reaction incubation. Such target-binding induced aptamer conformational change prevents the formation of C-Ag+-C structure, leaving more silver ions at the sample-membrane interface, which can be detected by the silver ISE. ATP can be quantified in the range of 0.5-3.0 mu M with a detection limit of 0.37 mu M. The relative standard deviation for 5 mu M ATP is 5.5%. For the proposed method, the combination of using ion fluxes of silver ions as modulating reagents and as signal reporters greatly simplifies the detection procedures. In addition, by changing the binding sequence in the middle of the probe, the present detection method will be able to explore new applications of ISE for the detection of a large variety of targets. (C) 2013 Elsevier B.V. All rights reserved.
学科主题Biophysics ; Biotechnology & Applied Microbiology ; Chemistry, Analytical ; Electrochemistry ; Nanoscience & Nanotechnology
URL标识查看原文
WOS关键词SMALL-MOLECULE ; LOGIC GATE ; LABEL-FREE ; OLIGONUCLEOTIDE ; PAIRS ; CYSTEINE ; DUPLEXES ; RELEASE ; COMPLEX ; SENSOR
WOS研究方向Biophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics
语种英语
WOS记录号WOS:000317888600024
资助机构Chinese Academy of Sciences [YZ201161]; National Natural Science Foundation of China [41176081, 21207156]; Science and Technology Project of Yantai [2012132]; Taishan Scholar Program of Shandong Province [TS20081159]
公开日期2013-08-15
源URL[http://ir.yic.ac.cn/handle/133337/6651]  
专题烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室
作者单位1.Chinese Acad Sci, Key Lab Coastal Zone Environm Processes & Ecol Re, Yantai Inst Coastal Zone Res YIC, Yantai 264003, Shandong, Peoples R China
2.YICCAS, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai 264003, Shandong, Peoples R China
3.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
推荐引用方式
GB/T 7714		 Ding, Jiawang,Qin, Wei,Zhang, Yan,et al. Potentiometric aptasensing based on target-induced conformational switch of a DNA probe using a polymeric membrane silver ion-selective electrode[J]. BIOSENSORS & BIOELECTRONICS,2013,45:148-151.
APA Ding, Jiawang,Qin, Wei,Zhang, Yan,&Wang, Xuewei.(2013).Potentiometric aptasensing based on target-induced conformational switch of a DNA probe using a polymeric membrane silver ion-selective electrode.BIOSENSORS & BIOELECTRONICS,45,148-151.
MLA Ding, Jiawang,et al."Potentiometric aptasensing based on target-induced conformational switch of a DNA probe using a polymeric membrane silver ion-selective electrode".BIOSENSORS & BIOELECTRONICS 45(2013):148-151.

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来源：烟台海岸带研究所

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