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  4. 海岸带生物学与生物资源利用重点实验室
  5. 海岸带生物学与生物资源利用所重点实验室
Regulation of Metabolites, Gene Expression, and Antioxidant Enzymes to Environmentally Relevant Lead and Zinc in the Halophyte Suaeda salsa

文献类型：期刊论文

作者Wu, Huifeng1; Liu, Xiaoli1,2; Zhao, Jianmin1; Yu, Junbao1
刊名JOURNAL OF PLANT GROWTH REGULATION
出版日期2013-06-01
卷号32期号:2页码:353-361
关键词Lead Zinc Biomarker Suaeda Salsa Metabolomics Antioxidant Enzyme
ISSN号0721-7595
产权排序[Wu, Huifeng; Liu, Xiaoli; Zhao, Jianmin; Yu, Junbao] Chinese Acad Sci, Key Lab Coastal Zone Environm Proc, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China; [Liu, Xiaoli] Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
通讯作者Wu, HF (reprint author), Chinese Acad Sci, Key Lab Coastal Zone Environm Proc, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China. hfwu@yic.ac.cn
文献子类Article
英文摘要As a pioneer halophyte, Suaeda salsa can grow in the intertidal zones, which are often polluted by heavy metals from both terrigenous wastewater and tidewater containing high concentrations of heavy metals. Therefore S. salsa is potentially suitable as a biomonitor for heavy-metal pollution in the intertidal zones. In this study, regulation of metabolites, gene expression, and antioxidant status of environmentally relevant lead and zinc were characterized using NMR-based metabolomics, real-time quantitative reverse transcription polymerase chain reaction, and antioxidant enzyme activities. In Pb-exposed S. salsa samples, only decreased tyrosine was observed, with statistical significance approaching 0.05. Metabolic biomarkers in Zn-exposed S. salsa samples included increased amino acids (valine, isoleucine, leucine, threonine, asparagine, and phenylalanine), and decreased acetate, glucose, ferulate, and fumarate. Increased succinate, aspartate, and malonate and decreased fructose were uniquely found in mixed Pb- and Zn-exposed samples in addition to the similar metabolic changes such as alanine, glucose, fumarate, and ferulate in Zn-exposed samples. Based on the metabolic biomarkers, gene expressions, and antioxidant enzyme activities, both Zn and mixed Pb and Zn induced significant oxidative stress and disturbances in energy metabolism, photosynthesis/glucogenesis, and protein biodegradation in S. salsa. However, environmentally relevant Pb could induce slight oxidative stress in S. salsa as indicated by increased catalase gene expression levels and catalase activities.; As a pioneer halophyte, Suaeda salsa can grow in the intertidal zones, which are often polluted by heavy metals from both terrigenous wastewater and tidewater containing high concentrations of heavy metals. Therefore S. salsa is potentially suitable as a biomonitor for heavy-metal pollution in the intertidal zones. In this study, regulation of metabolites, gene expression, and antioxidant status of environmentally relevant lead and zinc were characterized using NMR-based metabolomics, real-time quantitative reverse transcription polymerase chain reaction, and antioxidant enzyme activities. In Pb-exposed S. salsa samples, only decreased tyrosine was observed, with statistical significance approaching 0.05. Metabolic biomarkers in Zn-exposed S. salsa samples included increased amino acids (valine, isoleucine, leucine, threonine, asparagine, and phenylalanine), and decreased acetate, glucose, ferulate, and fumarate. Increased succinate, aspartate, and malonate and decreased fructose were uniquely found in mixed Pb- and Zn-exposed samples in addition to the similar metabolic changes such as alanine, glucose, fumarate, and ferulate in Zn-exposed samples. Based on the metabolic biomarkers, gene expressions, and antioxidant enzyme activities, both Zn and mixed Pb and Zn induced significant oxidative stress and disturbances in energy metabolism, photosynthesis/glucogenesis, and protein biodegradation in S. salsa. However, environmentally relevant Pb could induce slight oxidative stress in S. salsa as indicated by increased catalase gene expression levels and catalase activities.
学科主题Plant Sciences
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WOS关键词V-H+-ATPASE ; OSMOTIC-STRESS ; METABOLOMICS ; LEAVES ; NACL ; TOLERANCE ; EXPOSURE ; PPASE
WOS研究方向Plant Sciences
语种英语
WOS记录号WOS:000319165500012
资助机构Project of National Science and Technology Pillar Program [2011BAC02B01]; Chinese Academy of Sciences
公开日期2013-08-15
源URL[http://ir.yic.ac.cn/handle/133337/6661]  
专题烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室
烟台海岸带研究所_滨海湿地实验室
烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室
作者单位1.Chinese Acad Sci, Key Lab Coastal Zone Environm Proc, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
推荐引用方式
GB/T 7714		 Wu, Huifeng,Liu, Xiaoli,Zhao, Jianmin,et al. Regulation of Metabolites, Gene Expression, and Antioxidant Enzymes to Environmentally Relevant Lead and Zinc in the Halophyte Suaeda salsa[J]. JOURNAL OF PLANT GROWTH REGULATION,2013,32(2):353-361.
APA Wu, Huifeng,Liu, Xiaoli,Zhao, Jianmin,&Yu, Junbao.(2013).Regulation of Metabolites, Gene Expression, and Antioxidant Enzymes to Environmentally Relevant Lead and Zinc in the Halophyte Suaeda salsa.JOURNAL OF PLANT GROWTH REGULATION,32(2),353-361.
MLA Wu, Huifeng,et al."Regulation of Metabolites, Gene Expression, and Antioxidant Enzymes to Environmentally Relevant Lead and Zinc in the Halophyte Suaeda salsa".JOURNAL OF PLANT GROWTH REGULATION 32.2(2013):353-361.

入库方式： OAI收割

来源：烟台海岸带研究所

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