Non-homogeneous debris flows generally occur during the rainy seasons in Southwest China, and have received considerable attention in the literature. Regarding the complexity in debris flow dynamics, experimental approaches have proven to be effective in revealing the formative mechanism for debris flow, and quantifying the relations between the various influencing factors with debris-flow formation and subsequent transport processes. Therefore, a flume-based and experimental study was performed at the Debris Flow Observation and Research Station of Jiangjia Gully in Yunnan Province, to theoretically analyze favorable conditions for debris-flow formation and initial transport by selecting the median particle size d(50), flow rate Q, vertical grading coefficient y, slopes S, and the initial soil water contents W as the five variables for investigation. To achieve this, an optimal combination of these variables was made through an orthogonal experimental design to determine their relative importance upon the occurrence and initial mobilization behavior of a debris flow and to further enhance our insight into debris-flow triggering and transport mechanisms.