Surface invasive cleavage assay on a maskless light-directed diamond DNA microarray for genome-wide human SNP mapping
文献类型：期刊论文
| 作者 | Nie, Bei1,3; Yang, Min2; Fu, Weiling3; Liang, Zhiqing3 |
| 刊名 | ANALYST
 |
| 出版日期 | 2015 |
| 卷号 | 140期号:13页码:4549-4557 |
| ISSN号 | 0003-2654 |
| DOI | 10.1039/c5an00349k |
| 通讯作者 | Nie, B (reprint author), Chinese Acad Sci, Chongqing Inst Green & Intelligent Technol, 266 Fangzheng Ave, Chongqing 400714, Peoples R China. |
| 英文摘要 | The surface invasive cleavage assay, because of its innate accuracy and ability for self-signal amplification, provides a potential route for the mapping of hundreds of thousands of human SNP sites. However, its performance on a high density DNA array has not yet been established, due to the unusual "hairpin" probe design on the microarray and the lack of chemical stability of commercially available substrates. Here we present an applicable method to implement a nanocrystalline diamond thin film as an alternative substrate for fabricating an addressable DNA array using maskless light-directed photochemistry, producing the most chemically stable and biocompatible system for genetic analysis and enzymatic reactions. The surface invasive cleavage reaction, followed by degenerated primer ligation and post-rolling circle amplification is consecutively performed on the addressable diamond DNA array, accurately mapping SNP sites from PCR-amplified human genomic target DNA. Furthermore, a specially-designed DNA array containing dual probes in the same pixel is fabricated by following a reverse light-directed DNA synthesis protocol. This essentially enables us to decipher thousands of SNP alleles in a single-pot reaction by the simple addition of enzyme, target and reaction buffers. |
| 资助项目 | Chinese Academy of Sciences-Instrumental Innovation Program[Y29Z060Z10] ; 973 Program[2015CB755402] |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000356171400027 |
| 出版者 | ROYAL SOC CHEMISTRY |
| 源URL | [http://119.78.100.138/handle/2HOD01W0/1725]  |
| 专题 | 中国科学院重庆绿色智能技术研究院 |
| 通讯作者 | Nie, Bei |
| 作者单位 | 1.Chinese Acad Sci, Chongqing Inst Green & Intelligent Technol, Chongqing 400714, Peoples R China 2.Daping Hosp, Dept Gastroenterol, State Key Lab Trauma Burns & Combined Injury, Chongqing 400042, Peoples R China 3.Third Mil Med Univ, Southwest Hosp, Dept Lab Med, Chongqing 400038, Peoples R China |
| 推荐引用方式 GB/T 7714 | Nie, Bei,Yang, Min,Fu, Weiling,et al. Surface invasive cleavage assay on a maskless light-directed diamond DNA microarray for genome-wide human SNP mapping[J]. ANALYST,2015,140(13):4549-4557. |
| APA | Nie, Bei,Yang, Min,Fu, Weiling,&Liang, Zhiqing.(2015).Surface invasive cleavage assay on a maskless light-directed diamond DNA microarray for genome-wide human SNP mapping.ANALYST,140(13),4549-4557. |
| MLA | Nie, Bei,et al."Surface invasive cleavage assay on a maskless light-directed diamond DNA microarray for genome-wide human SNP mapping".ANALYST 140.13(2015):4549-4557. |
入库方式： OAI收割
来源：重庆绿色智能技术研究院
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