Nanopore label-free detection of single-nucleotide deletion in Bax/Bax2
文献类型：期刊论文
| 作者 | Chen, Xiaohan1; Wang, Liang2 ; Roozbahani, Golbarg M.1; Zhang, Youwen1; Xiang, Jialing3; Guan, Xiyun1
|
| 刊名 | ELECTROPHORESIS
 |
| 出版日期 | 2018-10-01 |
| 卷号 | 39期号:19页码:2410-2416 |
| 关键词 | Bax Genetic diagnosis Label-free Nanopore Nucleic acids |
| ISSN号 | 0173-0835 |
| DOI | 10.1002/elps.201800193 |
| 英文摘要 | Bax, a key tumor suppressor gene, will not be expressed correctly as a result of single nucleotide mutation in its microsatellite region; Instead, Bax2, an isoform of Bax, is often produced. In addition, lack of the exon 2 due to an alternative splicing, Bax2 has the same sequence as Bax except single base deletion from eight continuous guanines (G8) to G7. Most of the currently available methods for Bax2 detection are inefficient and time-consuming, and/or require the use of labels or dyes. In this work, we reported a label-free nanopore sensing strategy to differentiate between Bax and Bax2 with a DNA polymer as a molecular probe based on alternative spliced sequences. Two DNA molecules were designed to selectively detect Bax and Bax2, respectively. The method was rapid, accurate, and highly sensitive: picomolar concentrations of target nucleic acids could be detected in minutes. Our developed simple and fast nanopore-based detection strategy is not only useful for distinguishing between Bax and Bax2, but also provides a useful tool for detection of other single-base mutations in genetic diagnosis. |
| 资助项目 | National Institutes of Health[2R15GM110632-02] ; National Science Foundation[1708596] ; CAS Pioneer Hundred Talent Program |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000446272000004 |
| 出版者 | WILEY |
| 源URL | [http://119.78.100.138/handle/2HOD01W0/6837]  |
| 专题 | 中国科学院重庆绿色智能技术研究院 |
| 通讯作者 | Guan, Xiyun |
| 作者单位 | 1.IIT, Dept Chem, 3101 S Dearborn St, Chicago, IL 60616 USA 2.Chinese Acad Sci, Chongqing Inst Green & Intelligent Technol, Chongqing, Peoples R China 3.IIT, Dept Biol, Chicago, IL 60616 USA |
| 推荐引用方式 GB/T 7714 | Chen, Xiaohan,Wang, Liang,Roozbahani, Golbarg M.,et al. Nanopore label-free detection of single-nucleotide deletion in Bax/Bax2[J]. ELECTROPHORESIS,2018,39(19):2410-2416. |
| APA | Chen, Xiaohan,Wang, Liang,Roozbahani, Golbarg M.,Zhang, Youwen,Xiang, Jialing,&Guan, Xiyun.(2018).Nanopore label-free detection of single-nucleotide deletion in Bax/Bax2.ELECTROPHORESIS,39(19),2410-2416. |
| MLA | Chen, Xiaohan,et al."Nanopore label-free detection of single-nucleotide deletion in Bax/Bax2".ELECTROPHORESIS 39.19(2018):2410-2416. |
入库方式： OAI收割
来源：重庆绿色智能技术研究院
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