Raman Spectroscopy Analysis of the Biochemical Characteristics of Molecules Associated with the Malignant Transformation of Gastric Mucosa
文献类型：期刊论文
| 作者 | Chen, Yao1; Dai, Jianhua1; Zhou, Xueqian1; Liu, Yunjie1; Zhang, Wei2 ; Peng, Guiyong1
|
| 刊名 | PLOS ONE
 |
| 出版日期 | 2014-04-07 |
| 卷号 | 9期号:4页码:11 |
| ISSN号 | 1932-6203 |
| DOI | 10.1371/journal.pone.0093906 |
| 通讯作者 | Peng, GY (reprint author), Third Mil Med Univ, Southwest Hosp, Inst Gastroenterol, Chongqing, Peoples R China. |
| 英文摘要 | Objective: The purpose of this study was to comparatively analyze the signature Raman spectra of genomic DNA, nuclei, and tissue of normal gastric mucosa and gastric cancer and to investigate the biochemical transformation of molecules associated with gastric mucosa malignancy. Method: Genomic DNA, nuclei, and tissue from normal gastric mucosa and gastric cancer were analyzed by Raman spectroscopy. Results: 1) The Raman spectrum of gastric cancer genomic DNA showed that two peaks appeared, one at approximately 1090 cm(-1) with a higher intensity than the peak at 1050 cm(-1) in the spectrum. Characteristic peaks appeared at 950 cm-1, 1010 cm(- 1,) and 1100- 1600 cm(-1).2) Using a hematoxylin and eosin ( H& E)-stained section, the intensity of the characteristic peak of nucleic acids at 1085 cm(- 1) was increased and shifted to 1088 cm(- 1) in cancer cells. The relative intensity of the characteristic peaks of nucleoproteins at 755 cm (- 1) and 1607 cm (- 1) was significantly increased in cancer cells compared with normal cells. 3) Compared with normal tissues, the peak representing PO2- symmetric stretching vibration shifted from 1088 cm (- 1) to 1083 cm (- 1) in cancer tissue, and the characteristic peak for collagen at 938 cm (- 1) shifted to 944 cm (- 1). In addition, an extra characteristic peak indicating C = C stretching vibration appeared at 1379 cm (- 1) in the lipid spectrum in cancer tissue. Conclusions: The position, intensity, and shape of peaks in the Raman spectra of DNA, nuclei, and tissue from gastric cancer were significantly different compared with those of normal cells. These results indicate that the DNA phosphate backbone becomes unstable in cancer cells and might be broken; the relative content of histones is increased and stable; the relative collagen content is reduced, facilitating cancer cell metastasis; and the relative content of unsaturated fatty acids is increased, increasing the mobility of the plasma membrane of cancer cells. |
| 资助项目 | National Natural Science Foundation of China[81272580] |
| WOS研究方向 | Science & Technology - Other Topics |
| 语种 | 英语 |
| WOS记录号 | WOS:000334159800067 |
| 出版者 | PUBLIC LIBRARY SCIENCE |
| 源URL | [http://119.78.100.138/handle/2HOD01W0/1074]  |
| 专题 | 科研公共服务平台 |
| 通讯作者 | Peng, Guiyong |
| 作者单位 | 1.Third Mil Med Univ, Southwest Hosp, Inst Gastroenterol, Chongqing, Peoples R China 2.Chinese Acad Sci, Chongqing Inst Green & Intelligent Technol, Key Lab Multiscale Mfg Technol, Chongqing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, Yao,Dai, Jianhua,Zhou, Xueqian,et al. Raman Spectroscopy Analysis of the Biochemical Characteristics of Molecules Associated with the Malignant Transformation of Gastric Mucosa[J]. PLOS ONE,2014,9(4):11. |
| APA | Chen, Yao,Dai, Jianhua,Zhou, Xueqian,Liu, Yunjie,Zhang, Wei,&Peng, Guiyong.(2014).Raman Spectroscopy Analysis of the Biochemical Characteristics of Molecules Associated with the Malignant Transformation of Gastric Mucosa.PLOS ONE,9(4),11. |
| MLA | Chen, Yao,et al."Raman Spectroscopy Analysis of the Biochemical Characteristics of Molecules Associated with the Malignant Transformation of Gastric Mucosa".PLOS ONE 9.4(2014):11. |
入库方式： OAI收割
来源：重庆绿色智能技术研究院
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。