Dynamic Monitoring of MicroRNA-DNA Hybridization Using DNAase-Triggered Signal Amplification
文献类型：期刊论文
| 作者 | Qiu, Xiaopei1; Liu, Xing2; Zhang, Wei3 ; Zhang, Hong1; Jiang, Tianlun1; Fan, Dongli2; Luo, Yang1
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| 刊名 | ANALYTICAL CHEMISTRY
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| 出版日期 | 2015-06-16 |
| 卷号 | 87期号:12页码:6303-6310 |
| ISSN号 | 0003-2700 |
| DOI | 10.1021/acs.analchem.5b01159 |
| 通讯作者 | Luo, Y (reprint author), Third Mil Med Univ, Southwest Hosp, Dept Blood Transfus Med, Chongqing 400038, Peoples R China. |
| 英文摘要 | Dynamically monitoring microRNA (miRNA) DNA reactions is critical for elucidating various biological processes. However, traditional strategies fail to capture this dynamic event because the original targets are preamplified. In the present study, we developed an amplification-free strategy for real-time monitoring of miRNA DNA hybridization that integrates the advantages of both duplex-specific nuclease (DSN)-triggered signal amplification and single-stranded DNA probe coating facilitated by reduced graphene oxide. DSN-mediated miRNA recognition was found to consist of two phases: hybridization and hybridization cleavage. In the presence of miRNA and DSN, hybridization of a 22-mer miRNA DNA could be completed within 7 min by observing the angle increase in a surface plasmon resonance (SPR) biosensor. The subsequent hybridization-cleavage process could be visualized as a gradual SPR angle decrease that occurred until all coated probes were hydrolyzed. In addition, for miRNA-21 detection, the proposed linear signal amplification assay demonstrated a sensitivity of 3 fM over a dynamic range of S orders of magnitude. |
| 资助项目 | National Natural Science Foundation of China[81371899] ; Science Fund for Distinguished Young Scholars[CSTC2014JCYJJQ10007] ; Natural Science Foundation of Chongqing, China[CSTC-2013JJB10012] ; Twelfth Five-Year Plan of PLA, China[CWS13C046] ; China Academy of Engineering Physics[WSS-2014-09] ; Third Military Medical University of China[SWH2013LC13] |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000356755100061 |
| 出版者 | AMER CHEMICAL SOC |
| 源URL | [http://119.78.100.138/handle/2HOD01W0/1758]  |
| 专题 | 科研公共服务平台 |
| 通讯作者 | Luo, Yang |
| 作者单位 | 1.Third Mil Med Univ, Southwest Hosp, Dept Blood Transfus Med, Chongqing 400038, Peoples R China 2.Third Mil Med Univ, Xinqiao Hosp, Dept Plast Surg, Chongqing 400037, Peoples R China 3.Chinese Acad Sci, Chongqing Inst Green & Intelligent Technol, Chongqing 400714, Peoples R China |
| 推荐引用方式 GB/T 7714 | Qiu, Xiaopei,Liu, Xing,Zhang, Wei,et al. Dynamic Monitoring of MicroRNA-DNA Hybridization Using DNAase-Triggered Signal Amplification[J]. ANALYTICAL CHEMISTRY,2015,87(12):6303-6310. |
| APA | Qiu, Xiaopei.,Liu, Xing.,Zhang, Wei.,Zhang, Hong.,Jiang, Tianlun.,...&Luo, Yang.(2015).Dynamic Monitoring of MicroRNA-DNA Hybridization Using DNAase-Triggered Signal Amplification.ANALYTICAL CHEMISTRY,87(12),6303-6310. |
| MLA | Qiu, Xiaopei,et al."Dynamic Monitoring of MicroRNA-DNA Hybridization Using DNAase-Triggered Signal Amplification".ANALYTICAL CHEMISTRY 87.12(2015):6303-6310. |
入库方式： OAI收割
来源：重庆绿色智能技术研究院
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