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Detection of DNA oligonucleotides with base mutations by terahertz spectroscopy and microstructures
文献类型：期刊论文
| 作者 | Tang, Mingjie1 ; Zhang, Mingkun1; Yan, Shihan1; Xia, Liangping1; Yang, Zhongbo1; Du, Chunlei1; Cui, Hong-Liang1,2; Wei, Dongshan1
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| 刊名 | PLOS ONE
 |
| 出版日期 | 2018-01-24 |
| 卷号 | 13期号:1页码:12 |
| ISSN号 | 1932-6203 |
| DOI | 10.1371/journal.pone.0191515 |
| 英文摘要 | DNA oligonucleotides with a 5-base mutation at the 3'-terminus were investigated by terahertz (THz) spectroscopy in a marker-free manner. The four single-stranded oligonucleotides with 17nt have been detected with specificity on a microfluidic chip, and corroborated by spectral measurements with split-ring resonators. The number of hydrogen bonds formed between the oligonucleotide and its surrounding water molecules, deemed a key contribution to the THz absorption of biological solutions, was explored by molecular dynamics simulations to explain the experimental findings. Our work underlies the feasibility of THz spectroscopy combined with microstructures for marker-free detection of DNA, which may form the basis of a prospective diagnostic tool for studying genic mutation. |
| 资助项目 | National 973 Program of China[2015CB755401] ; National Natural Science Foundation of China[21407145] ; National Natural Science Foundation of China[31400625] ; National Natural Science Foundation of China[61605206] ; National Key Research and Development Program of China[2017YFF0106303] ; National Key Research and Development Program of China[2016YFC0101300] ; National Key Research and Development Program of China[2016YFC0101002] |
| WOS研究方向 | Science & Technology - Other Topics |
| 语种 | 英语 |
| 出版者 | PUBLIC LIBRARY SCIENCE |
| WOS记录号 | WOS:000423412500055 |
| 源URL | [http://119.78.100.138/handle/2HOD01W0/6241]  |
| 专题 | 太赫兹技术研究中心 微纳制造与系统集成研究中心 |
| 通讯作者 | Wei, Dongshan |
| 作者单位 | 1.Chinese Acad Sci, Chongqing Key Lab Multiscale Mfg Technol, Chongqing Inst Green & Intelligent Technol, Chongqing, Peoples R China 2.Jilin Univ, Coll Instrumentat Sci & Elect Engn, Changchun, Jilin, Peoples R China |
| 推荐引用方式 GB/T 7714 | Tang, Mingjie,Zhang, Mingkun,Yan, Shihan,et al. Detection of DNA oligonucleotides with base mutations by terahertz spectroscopy and microstructures[J]. PLOS ONE,2018,13(1):12. |
| APA | Tang, Mingjie.,Zhang, Mingkun.,Yan, Shihan.,Xia, Liangping.,Yang, Zhongbo.,...&Wei, Dongshan.(2018).Detection of DNA oligonucleotides with base mutations by terahertz spectroscopy and microstructures.PLOS ONE,13(1),12. |
| MLA | Tang, Mingjie,et al."Detection of DNA oligonucleotides with base mutations by terahertz spectroscopy and microstructures".PLOS ONE 13.1(2018):12. |
入库方式： OAI收割
来源：重庆绿色智能技术研究院
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