Endocytic Fluorescence of Nanodiamond in Living Hela Cells Determined by Microscopy Imaging Techniques
文献类型：期刊论文
| 作者 | Zheng, Mei-Ling1,2; Lei, Ying1,2,5; Zheng, Yong-Chao1,2,5; Xu, Tao4,5; Zhi, Jin-Fang4; Zhao, Zhen-Sheng1,2; Duan, Xuan-Ming1,2,3 |
| 刊名 | JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY
 |
| 出版日期 | 2016-07-01 |
| 卷号 | 16期号:7页码:6748-6754 |
| 关键词 | Fluorescence Enhancement Nanodiamond Microscopy Living Cell Imaging |
| ISSN号 | 1533-4880 |
| DOI | 10.1166/jnn.2016.11307 |
| 通讯作者 | Zheng, ML ; Duan, XM (reprint author), Chinese Acad Sci, Lab Organ NanoPhoton, 29 Zhongguancun East Rd, Beijing 100190, Peoples R China. ; Zheng, ML ; Duan, XM (reprint author), Chinese Acad Sci, Key Lab Funct Crystals & Laser Technol, Tech Inst Phys & Chem, 29 Zhongguancun East Rd, Beijing 100190, Peoples R China. ; Duan, XM (reprint author), Chinese Acad Sci, Chongqing Inst Green & Intelligent Technol, Shuitu Technol Dev Zone, 266 Fangzheng Ave, Chongqing 400714, Peoples R China. |
| 英文摘要 | We have prepared the carboxylate nanodiamond (ND-COOH) and demonstrated the enhanced endocytic fluorescence in living Hela cells by using confocal fluorescence microscopy imaging and transmission electron microscopy (TEM) techniques. The fluorescence enhancement of ND-COOH is observed during the staining dynamics of living cells using confocal fluorescence microscopy. Furthermore, the intracellular localization of ND-COOH in endocytic cells is verified by TEM and the three-dimensional (3D) optical sectioning image of confocal fluorescence microscopy. The low cytotoxicity and high photostability have also been clearly demonstrated. The characterization and the analysis of microscopy provide the unique direct evidence that the ND-COOH can enter living cells though the endocytosis process and exhibits strong endocytic fluorescence. This study would open up new avenues towards the application of functional ND in living cell labelling or drug delivery system. |
| 资助项目 | National Natural Science Foundation of China[61475164] ; National Natural Science Foundation of China[91323301] ; National Natural Science Foundation of China[61205194] ; National Natural Science Foundation of China[21375137] ; National Natural Science Foundation of China[91123032] ; International Cooperation Program of MOST[2010DFA01180] ; CAS-JSPS Joint Research Project[GJHZ1411] |
| WOS研究方向 | Chemistry ; Science & Technology - Other Topics ; Materials Science ; Physics |
| 语种 | 英语 |
| WOS记录号 | WOS:000387100400018 |
| 出版者 | AMER SCIENTIFIC PUBLISHERS |
| 源URL | [http://119.78.100.138/handle/2HOD01W0/3009]  |
| 专题 | 机器人与3D打印技术创新中心 |
| 通讯作者 | Zheng, Mei-Ling; Duan, Xuan-Ming |
| 作者单位 | 1.Chinese Acad Sci, Lab Organ NanoPhoton, 29 Zhongguancun East Rd, Beijing 100190, Peoples R China 2.Chinese Acad Sci, Key Lab Funct Crystals & Laser Technol, Tech Inst Phys & Chem, 29 Zhongguancun East Rd, Beijing 100190, Peoples R China 3.Chinese Acad Sci, Chongqing Inst Green & Intelligent Technol, Shuitu Technol Dev Zone, 266 Fangzheng Ave, Chongqing 400714, Peoples R China 4.Chinese Acad Sci, Key Lab Photochem Convers & Optoelect Mat, Tech Inst Phys & Chem, 29 Zhongguancun East Rd, Beijing 100190, Peoples R China 5.Univ Chinese Acad Sci, 29 Zhongguancun East Rd, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zheng, Mei-Ling,Lei, Ying,Zheng, Yong-Chao,et al. Endocytic Fluorescence of Nanodiamond in Living Hela Cells Determined by Microscopy Imaging Techniques[J]. JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY,2016,16(7):6748-6754. |
| APA | Zheng, Mei-Ling.,Lei, Ying.,Zheng, Yong-Chao.,Xu, Tao.,Zhi, Jin-Fang.,...&Duan, Xuan-Ming.(2016).Endocytic Fluorescence of Nanodiamond in Living Hela Cells Determined by Microscopy Imaging Techniques.JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY,16(7),6748-6754. |
| MLA | Zheng, Mei-Ling,et al."Endocytic Fluorescence of Nanodiamond in Living Hela Cells Determined by Microscopy Imaging Techniques".JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY 16.7(2016):6748-6754. |
入库方式： OAI收割
来源：重庆绿色智能技术研究院
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