Label-free sensing of the binding state of MUC1 peptide and anti-MUC1 aptamer solution in fluidic chip by terahertz spectroscopy
文献类型：期刊论文
| 作者 | Zhao, Xiang1; Zhang, Mingkun2 ; Wei, Dongshan2; Wang, Yunxia1; Yan, Shihan2; Liu, Mengwan2; Yang, Xiang1; Yang, Ke1; Cui, Hong-Liang2; Fu, Weiling1
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| 刊名 | BIOMEDICAL OPTICS EXPRESS
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| 出版日期 | 2017-10-01 |
| 卷号 | 8期号:10页码:4427-4437 |
| ISSN号 | 2156-7085 |
| DOI | 10.1364/BOE.8.004427 |
| 通讯作者 | Cui, HL (reprint author), Chinese Acad Sci, Chongqing Inst Green & Intelligent Technol, Chongqing Key Lab Multiscale Mfg Technol, Chongqing 400714, Peoples R China. |
| 英文摘要 | The aptamer and target molecule binding reaction has been widely applied for construction of aptasensors, most of which are labeled methods. In contrast, terahertz technology proves to be a label-free sensing tool for biomedical applications. We utilize terahertz absorption spectroscopy and molecular dynamics simulation to investigate the variation of binding-induced collective vibration of hydrogen bond network in a mixed solution of MUC1 peptide and anti-MUC1 aptamer. The results show that binding-induced alterations of hydrogen bond numbers could be sensitively reflected by the variation of terahertz absorption coefficients of the mixed solution in a customized fluidic chip. The minimal detectable concentration is determined as 1 pmol/mu L, which is approximately equal to the optimal immobilized concentration of aptasensors. (C) 2017 Optical Society of America |
| 资助项目 | National Basic Research Program of China (Program 973)[2015CB755400] ; National Natural Science Foundation of China (NSFC)[81430054] ; National Natural Science Foundation of China (NSFC)[81370049] ; National Natural Science Foundation of China (NSFC)[61605206] ; National Natural Science Foundation of China (NSFC)[31400625] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Optics ; Radiology, Nuclear Medicine & Medical Imaging |
| 语种 | 英语 |
| WOS记录号 | WOS:000412052000010 |
| 出版者 | OPTICAL SOC AMER |
| 源URL | [http://172.16.51.4:88/handle/2HOD01W0/144]  |
| 专题 | 太赫兹技术研究中心 |
| 通讯作者 | Cui, Hong-Liang |
| 作者单位 | 1.Third Mil Med Univ, Southwest Hosp, Dept Lab Med, Chongqing 400038, Peoples R China 2.Chinese Acad Sci, Chongqing Inst Green & Intelligent Technol, Chongqing Key Lab Multiscale Mfg Technol, Chongqing 400714, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao, Xiang,Zhang, Mingkun,Wei, Dongshan,et al. Label-free sensing of the binding state of MUC1 peptide and anti-MUC1 aptamer solution in fluidic chip by terahertz spectroscopy[J]. BIOMEDICAL OPTICS EXPRESS,2017,8(10):4427-4437. |
| APA | Zhao, Xiang.,Zhang, Mingkun.,Wei, Dongshan.,Wang, Yunxia.,Yan, Shihan.,...&Fu, Weiling.(2017).Label-free sensing of the binding state of MUC1 peptide and anti-MUC1 aptamer solution in fluidic chip by terahertz spectroscopy.BIOMEDICAL OPTICS EXPRESS,8(10),4427-4437. |
| MLA | Zhao, Xiang,et al."Label-free sensing of the binding state of MUC1 peptide and anti-MUC1 aptamer solution in fluidic chip by terahertz spectroscopy".BIOMEDICAL OPTICS EXPRESS 8.10(2017):4427-4437. |
入库方式： OAI收割
来源：重庆绿色智能技术研究院
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