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Intracellular localisation of proteins to specific cellular areas by nanocapsule mediated delivery

文献类型：期刊论文

作者Wang, Huabin1,2; Chen, Ligang1,2; Sun, Xianchao1; Fu, Ailing1
刊名JOURNAL OF DRUG TARGETING
出版日期2017
卷号25期号:8页码:724-733
关键词Nanocapsule protein delivery intracellular localisation fluorescence
ISSN号1061-186X
DOI10.1080/1061186X.2017.1323908
通讯作者Fu, AL (reprint author), Southwest Univ, Sch Pharmaceut Sci, 2 Tian Sheng Rd, Chongqing 400716, Peoples R China.
英文摘要Nanocapsules are promising carriers with great potential for intracellular protein transport. Although many studies have intended to improve cell uptake efficacy, there is an increasing interest in understanding of subcellular distribution of cargoes inside cells, which is essential for purposeful delivery of biomolecules into specific sites within cells. Herein, we interrogate the intracellular localisation of exogenous proteins, including fluorescein isothiocyanate (FITC)-labelled bovine serum albumin (BSA) and green fluorescent protein (GFP), mediated by specially designed nanocapsules. The results show that the designed nanocapsules can deliver the two types of fluorescent proteins into different cellular destinations (cytosol, nucleus or the whole cell), depending on the composition of nanocapsules. Meanwhile, several impact factors that influence the distribution of proteins in cells have also been investigated, and the results suggest that the localisation of capsule-mediated proteins in cells is strongly affected by the surface properties of nanocapsules, the types of stabilisers and proteins, and environmental temperatures. The rational control of intracellular localised delivery of exogenous proteins as we demonstrated in this study might open new avenues to obtain desired magnitude of drug effects for modulating cell activity.
资助项目Natural Science Foundation of China[81273416] ; Scientific Research Foundation for Returned Scholars, Ministry of Education of China[2012-940] ; Fundamental Research Funds for the Central Universities[XDJK2013A030] ; Program for New Century Excellent Talents in University, Ministry of Education of China ; Chongqing Institute of Green and Intelligent Technology for Startup Foundation[cstc2015jcyjiA10057] ; Chongqing Institute of Green and Intelligent Technology for Startup Foundation[YJ500061LH1] ; Chinese Academy of Sciences[R52A500Z10]
WOS研究方向Pharmacology & Pharmacy
语种英语
WOS记录号WOS:000404800400006
出版者TAYLOR & FRANCIS LTD
源URL[http://172.16.51.4:88/handle/2HOD01W0/288]  
专题太赫兹技术研究中心
通讯作者Fu, Ailing
作者单位1.Southwest Univ, Sch Pharmaceut Sci, 2 Tian Sheng Rd, Chongqing 400716, Peoples R China
2.Chinese Acad Sci, Chongqing Inst Green & Intelligent Technol, Chongqing Key Lab Multiscale Mfg Technol, Chongqing, Peoples R China
推荐引用方式
GB/T 7714		 Wang, Huabin,Chen, Ligang,Sun, Xianchao,et al. Intracellular localisation of proteins to specific cellular areas by nanocapsule mediated delivery[J]. JOURNAL OF DRUG TARGETING,2017,25(8):724-733.
APA Wang, Huabin,Chen, Ligang,Sun, Xianchao,&Fu, Ailing.(2017).Intracellular localisation of proteins to specific cellular areas by nanocapsule mediated delivery.JOURNAL OF DRUG TARGETING,25(8),724-733.
MLA Wang, Huabin,et al."Intracellular localisation of proteins to specific cellular areas by nanocapsule mediated delivery".JOURNAL OF DRUG TARGETING 25.8(2017):724-733.

入库方式： OAI收割

来源：重庆绿色智能技术研究院

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