Biosensing of BCR/ABL fusion gene using an intensity-interrogation surface plasmon resonance imaging system
文献类型：期刊论文
| 作者 | Wu, Jiangling1; Huang, Yu1,2 ; Bian, Xintong; Li, DanDan1; Cheng, Quan1,3; Ding, Shijia1
|
| 刊名 | OPTICS COMMUNICATIONS
 |
| 出版日期 | 2016-10-15 |
| 卷号 | 377页码:24-32 |
| 关键词 | Surface plasmon resonance imaging Gold microarray DNA hybridization BCR/ABL fusion gene |
| ISSN号 | 0030-4018 |
| DOI | 10.1016/j.optcom.2016.05.035 |
| 通讯作者 | Ding, SJ (reprint author), Chongqing Med Univ, Coll Lab Med, Key Lab Clin Lab Diagnost, Minist Educ, Chongqing 400016, Peoples R China. |
| 英文摘要 | In this work, a custom-made intensity-interrogation surface plasmon resonance imaging (SPRi) system has been developed to directly detect a specific sequence of BCR/ABL fusion gene in chronic myelogenous leukemia (CML). The variation in the reflected light intensity detected from the sensor chip composed of gold islands array is proportional to the change of refractive index due to the selective hybridization of surface-bound DNA probes with target ssDNA. SPRi measurements were performed with different concentrations of synthetic target DNA sequence. The calibration curve of synthetic target sequence shows a good relationship between the concentration of synthetic target and the change of reflected light intensity. The detection limit of this SPRi measurement could approach 10.29 nM. By comparing SPRi images, the target ssDNA and non-complementary DNA sequence are able to be distinguished. This SPRi system has been applied for assay of BCR/ABL fusion gene extracted from real samples. This nucleic acid based SPRi biosensor therefore offers an alternative high-effective, high-throughput label-free tool for DNA detection in biomedical research and molecular diagnosis. (C) 2016 Elsevier B.V. All rights reserved. |
| URL标识 | 查看原文 ; 查看原文 |
| 资助项目 | Achievement Transfer Project of Institutions of Higher Education in Chongqing[KJZH14205] ; National Natural Science Foundation of China (NSFC)[61307103] ; Application Development Plan Project of Chongqing[cstc2014yykfB10003] ; Natural Science Foundation Project of Chongqing[cstc2014kjrc-qnrc10001] |
| WOS研究方向 | Optics |
| 语种 | 英语 |
| WOS记录号 | WOS:000377397600004 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://119.78.100.138/handle/2HOD01W0/2550]  |
| 专题 | 太赫兹技术研究中心 |
| 通讯作者 | Ding, Shijia |
| 作者单位 | 1.Chongqing Med Univ, Coll Lab Med, Key Lab Clin Lab Diagnost, Minist Educ, Chongqing 400016, Peoples R China 2.Chinese Acad Sci, Chongqing Inst Green & Intelligent Technol, Chongqing 400714, Peoples R China 3.Univ Calif Riverside, Dept Chem, Riverside, CA 92521 USA |
| 推荐引用方式 GB/T 7714 | Wu, Jiangling,Huang, Yu,Bian, Xintong,et al. Biosensing of BCR/ABL fusion gene using an intensity-interrogation surface plasmon resonance imaging system[J]. OPTICS COMMUNICATIONS,2016,377:24-32. |
| APA | Wu, Jiangling,Huang, Yu,Bian, Xintong,Li, DanDan,Cheng, Quan,&Ding, Shijia.(2016).Biosensing of BCR/ABL fusion gene using an intensity-interrogation surface plasmon resonance imaging system.OPTICS COMMUNICATIONS,377,24-32. |
| MLA | Wu, Jiangling,et al."Biosensing of BCR/ABL fusion gene using an intensity-interrogation surface plasmon resonance imaging system".OPTICS COMMUNICATIONS 377(2016):24-32. |
入库方式： OAI收割
来源：重庆绿色智能技术研究院
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