Rapid discrimination of DNA sequences using an engineered nanopore
文献类型：会议论文
| 作者 | Ying, Cuifeng1,3; Feng, Yanxiao1; Wang, Deqiang1 ; Guan, Xiyun2; Zhou, Wenyuan3; Tian, Jianguo3; Zhang, Yuechuan4
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| 出版日期 | 2015 |
| 会议日期 | October 5, 2015 - October 9, 2015 |
| 会议地点 | Changchun, China |
| DOI | 10.1109/3M-NANO.2015.7425464 |
| 页码 | 272-277 |
| 英文摘要 | Nanopores have been used for the development of biosensors for various substances and are currently under intense investigation as a potential tool for the entire genome sequencing. It has been demonstrated that an α-hemolysin protein pore covalently attached with a β-cyclodextrin adapter can differentiate single bases[1]; furthermore, single base discrimination could be achieved when a single-stranded DNA (ssDNA) is immobilized in the α-hemolysin pore[2]. Here, we show that when an unlabelled/unmodified double-stranded DNA (dsDNA) translocates through a properly engineered α-hemolysinnanopore, a sequence of sub-state blockage events is observed. Since different sequences of sub-state modulation events are produced by different DNA strands, they offer the potential as characteristic fingerprints to identify and discriminate the precise sequences of dsDNA samples. © 2015 IEEE. |
| 会议录 | 5th International Conference on Manipulation, Manufacturing and Measurement on the Nanoscale, 3M-NANO 2015
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| 语种 | 英语 |
| 源URL | [http://119.78.100.138/handle/2HOD01W0/4818]  |
| 专题 | 精准医疗单分子诊断技术研究中心 |
| 作者单位 | 1.Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Chongqing, China; 2.Department of Biological and Chemical Sciences, Illinois Institute of Technology, Chicago, United States; 3.Key Laboratory of Weak-Light Nonlinear Photonics, Ministry of Education, School of Physics, Nankai University, Tianjin, China; 4.University of Electronic Science and Technology of China, Chengdu, China |
| 推荐引用方式 GB/T 7714 | Ying, Cuifeng,Feng, Yanxiao,Wang, Deqiang,et al. Rapid discrimination of DNA sequences using an engineered nanopore[C]. 见:. Changchun, China. October 5, 2015 - October 9, 2015. |
入库方式： OAI收割
来源：重庆绿色智能技术研究院
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