Manure fertilization alters the population of ammonia-oxidizing bacteria rather than ammonia-oxidizing archaea in a paddy soil
文献类型：期刊论文
| 作者 | Wang, Yu1,2; Zhu, Guibing2; Song, Liyan1 ; Wang, Shanyun2,3; Yin, Chengqing2
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| 刊名 | JOURNAL OF BASIC MICROBIOLOGY
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| 出版日期 | 2014-03-01 |
| 卷号 | 54期号:3页码:190-197 |
| 关键词 | Ammonia-oxidizing archaea Ammonia-oxidizing bacteria Manure fertilization Paddy soil |
| ISSN号 | 0233-111X |
| DOI | 10.1002/jobm.201200671 |
| 通讯作者 | Zhu, GB (reprint author), Chinese Acad Sci, State Key Lab Environm Aquat Qual, Res Ctr Ecoenvironm Sci, POB 2871,Shuangqing Rd, Beijing 100085, Peoples R China. |
| 英文摘要 | Manure fertilizers are widely used in agriculture and highly impacted the soil microbial communities such as ammonia oxidizers. However, the knowledge on the communities of archaeal versus bacterial ammonia oxidizers in paddy soil affected by manure fertilization remains largely unknown, especially for a long-term influence. In present work, the impact of manure fertilization on the population of ammonia oxidizers, related potential nitrification rates (PNRs) and the key factors manipulating the impact were investigated through studying two composite soil cores (long-term fed with manure fertilization versus undisturbed). Moreover, soil incubated with NH4+ for 5 weeks was designed to verify the field research. The results showed that the copy numbers of bacterial amoA gene in the manure fed soil were significant higher than those in the unfed soil (p<0.05), suggesting a clear stimulating effect of long-term manure fertilization on the population of ammonia-oxidizing bacteria (AOB). The detected PNRs in the manure fed soil core (14-218nmolL(-1)Ng(-1)h(-1)) were significant higher than those in the unfed soil core (5-72nmolL(-1)Ng(-1)h(-1); p<0.05). Highly correlations between the PNRs and the bacterial amoA gene copies rather than archaeal amoA gene were observed, indicating strong nitrification capacity related to bacterial ammonia oxidizers. The NH4+-N significantly correlated to the abundance of AOB (p<0.01) and explained 96.1% of the environmental variation, showing the NH4+-N was the main factor impacting the population of AOB. The incubation experiment demonstrated a clear increase of the bacterial amoA gene abundance (2.0x10(6) to 8.4x10(6)g(-1)d.w.s. and 1.6x10(4) to 4.8x10(5)g(-1)d.w.s.) in both soil but not for the archaeal amoA gene, in agreement with the field observation. Overall, our results suggested that manure fertilization promoted the population size of bacterial ammonia oxidizers rather than their archaeal counterparts whether in long-term or short-term usage and the NH4+-N was the key impact factor. |
| URL标识 | 查看原文 |
| 资助项目 | National Natural Science Foundation of China[21277156] ; Knowledge Innovation Program of the Chinese Academy of Sciences[KZCX2-EW-410-01] ; special fund of State Key Joint Laboratory of Environment Simulation and Pollution Control[12L03ESPC] ; Beijing Nova Program[2011095] ; K. C. Wong Education Foundation, Hong Kong |
| WOS研究方向 | Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000332082500004 |
| 出版者 | WILEY-BLACKWELL |
| 源URL | [http://119.78.100.138/handle/2HOD01W0/821]  |
| 专题 | 生态过程与重建研究中心 中国科学院重庆绿色智能技术研究院 |
| 通讯作者 | Zhu, Guibing |
| 作者单位 | 1.Chinese Acad Sci, Chongqing Inst Green & Intelligent Technol, Chongqing, Peoples R China 2.Chinese Acad Sci, State Key Lab Environm Aquat Qual, Res Ctr Ecoenvironm Sci, Beijing 100085, Peoples R China 3.Harbin Inst Technol, State Key Lab Urban Water Resource & Environm, Harbin 150006, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Yu,Zhu, Guibing,Song, Liyan,et al. Manure fertilization alters the population of ammonia-oxidizing bacteria rather than ammonia-oxidizing archaea in a paddy soil[J]. JOURNAL OF BASIC MICROBIOLOGY,2014,54(3):190-197. |
| APA | Wang, Yu,Zhu, Guibing,Song, Liyan,Wang, Shanyun,&Yin, Chengqing.(2014).Manure fertilization alters the population of ammonia-oxidizing bacteria rather than ammonia-oxidizing archaea in a paddy soil.JOURNAL OF BASIC MICROBIOLOGY,54(3),190-197. |
| MLA | Wang, Yu,et al."Manure fertilization alters the population of ammonia-oxidizing bacteria rather than ammonia-oxidizing archaea in a paddy soil".JOURNAL OF BASIC MICROBIOLOGY 54.3(2014):190-197. |
入库方式： OAI收割
来源：重庆绿色智能技术研究院
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