Nitrite-dependent anaerobic methane oxidizing bacteria along the water level fluctuation zone of the Three Gorges Reservoir
文献类型：期刊论文
| 作者 | Wang, Yu1; Huang, Pei1; Ye, Fei1; Jiang, Yi1 ; Song, Liyan1; Op den Camp, Huub J. M.2; Zhu, Guibing3; Wu, Shengjun1
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| 刊名 | APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
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| 出版日期 | 2016-02-01 |
| 卷号 | 100期号:4页码:1977-1986 |
| 关键词 | Nitrite-dependent anaerobic methane oxidation M. oxyfera-like bacteria Water level fluctuation zone Three gorges reservoir Water flooding |
| ISSN号 | 0175-7598 |
| DOI | 10.1007/s00253-015-7083-2 |
| 通讯作者 | Wu, SJ (reprint author), Chinese Acad Sci, Chongqing Inst Green & Intelligent Technol, Chongqing 400714, Peoples R China. |
| 英文摘要 | The nitrite-dependent anaerobic methane oxidation (n-damo) mediated by "Candidatus Methylomirabilis oxyfera" connects the biogeochemical carbon and nitrogen cycles in a novel way. Many environments have been reported to harbor such organism being slow-growing and oxygen-sensitive anaerobes. Here, we focused on the population of n-damo bacteria in a fluctuating habitat being the wetland in the water level fluctuation zone (WLFZ) of the Three Gorges Reservoir (TGR) in China. A molecular approach demonstrated positive amplifications when targeting the functional pmoA gene only in the lower sites which endured longer flooding time in an elevation gradient. Only 1 operational taxonomic unit (OTU) in the lower elevation zone targeting the 16S ribosomal RNA (rRNA) gene was clustering into the NC-10 group a, which is presumed to be the true n-damo group. Moreover, a relatively low level of diversity was observed in this study. The abundances were as low as 4.7 x 10(2) to 1.5 x 10(3) copies g(-1) dry soil (ds) in the initial stage, which were almost the lowest reported. However, an increase was observed (3.2 x 10(3) to 5.3 x 10(4) copies g(-1) ds) after nearly 6 months of flooding. Intriguingly, the abundance of n-damo bacteria correlated positively with the accumulated flooding time (AFT). The current study revealed that n-damo bacteria can be detected in a fluctuating environment and the sites with longer flooding time seem to be preferred habitats. The water flooding may be the principal factor in this ecosystem by creating anoxic condition. The wide range of such habitats suggests a high potential of n-damo bacteria to play a key role in natural CH4 consumption. |
| URL标识 | 查看原文 |
| 资助项目 | National Natural Science Foundation of China[41303053] ; Fundamental and Frontier Research Project of Chongqing[cstc2013jcyjA20003] ; West Light Foundation ; West China Action Plan of the Chinese Academy of Sciences[KZCX2-XB3-14-01] |
| WOS研究方向 | Biotechnology & Applied Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000369309000039 |
| 出版者 | SPRINGER |
| 源URL | [http://119.78.100.138/handle/2HOD01W0/2210]  |
| 专题 | 生态过程与重建研究中心 中国科学院重庆绿色智能技术研究院 |
| 通讯作者 | Wu, Shengjun |
| 作者单位 | 1.Chinese Acad Sci, Chongqing Inst Green & Intelligent Technol, Chongqing 400714, Peoples R China 2.Radboud Univ Nijmegen, IWWR, Dept Microbiol, NL-6525 AJ Nijmegen, Netherlands 3.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, Key Lab Drinking Water Sci & Technol, Beijing 100085, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Yu,Huang, Pei,Ye, Fei,et al. Nitrite-dependent anaerobic methane oxidizing bacteria along the water level fluctuation zone of the Three Gorges Reservoir[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2016,100(4):1977-1986. |
| APA | Wang, Yu.,Huang, Pei.,Ye, Fei.,Jiang, Yi.,Song, Liyan.,...&Wu, Shengjun.(2016).Nitrite-dependent anaerobic methane oxidizing bacteria along the water level fluctuation zone of the Three Gorges Reservoir.APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,100(4),1977-1986. |
| MLA | Wang, Yu,et al."Nitrite-dependent anaerobic methane oxidizing bacteria along the water level fluctuation zone of the Three Gorges Reservoir".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 100.4(2016):1977-1986. |
入库方式： OAI收割
来源：重庆绿色智能技术研究院
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