Generation and application of a novel transgenic zebrafish line Tg(cyp1a:mCherry) as an in vivo assay to sensitively monitor PAHs and TCDD in the environment
文献类型：期刊论文
| 作者 | Xie, Shao-Lin1,2; Junaid, Muhammad1,3; Bian, Wan-Ping1,3 ; Luo, Juan-Juan1,4; Syed, Jabir Hussain5; Wang, Chao1,2; Xiong, Wen-Xu1; Ma, Yan-Bo1,3; Niu, Aping1,3; Yang, Xiao-Jun4
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| 刊名 | JOURNAL OF HAZARDOUS MATERIALS
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| 出版日期 | 2018-02-15 |
| 卷号 | 344页码:723-732 |
| ISSN号 | 0304-3894 |
| 关键词 | 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) cyp1a promoter Environmental level Polycyclic aromatic hydrocarbons (PAHs) Zebrafish |
| DOI | 10.1016/j.jhazmat.2017.11.021 |
| 英文摘要 | The polycyclic aromatic hydrocarbons (PAHs) and 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) are classified as human carcinogens, and can also cause serious health problems. To develop a convenient bio-monitoring tool for the detection of PAHs and TCDD in the environment, we generated a transgenic zebrafish line Tg(cyp1a:mCherry) with cyp1a promoter driving mCherry expression. Here, Tg(cyp1a:mCherry) embryos were treated with different concentrations of TCDD and five US EPA priority PAHs congeners. The results showed that the expressions of mCherry and endogenous cyp1a were consistent with the PAHs exposure concentrations and were largely induced by TCDD and >= 4-ring PAHs. Moreover, the sensitivity of Tg(cyp1a:mCherry) embryos was also evaluated through monitoring of the PAHs contamination in the water and soil samples. The elevated red fluorescent signals and cyp1a expression levels were observed in Tg(cyp1a:mCherry)zebrafish after exposure to water samples and soil organic extracts with higher concentrations of >= 4-ring PAHs. These results further strengthen our findings of concentration- and congener-dependent response of the newly established zebrafish. Taken together, the newly established zebrafish line will prove as a sensitive, efficient and convenient tool for monitoring PAHs and TCDD contamination in the environment. (C) 2017 Elsevier B.V. All rights reserved. |
| 资助项目 | Three Hundred Leading Talents in Scientific and Technological Innovation Program of Chongqing[CSTCCXLJRC201714] ; Key Application and Development Program of Chongqing Science and Technology Commission[cstc2014yykfC20004] ; Key Application and Development Program of Chongqing Science and Technology Commission[cstc2014yykfC20002] ; National Natural Science Foundation of China[21407146] ; National Natural Science Foundation of China[51641907] |
| WOS研究方向 | Engineering ; Environmental Sciences & Ecology |
| 语种 | 英语 |
| 出版者 | ELSEVIER SCIENCE BV |
| WOS记录号 | WOS:000423246700073 |
| 源URL | [http://119.78.100.138/handle/2HOD01W0/4523]  |
| 专题 | 环境与健康研究中心 |
| 通讯作者 | Zou, Ji-Xing; Pei, De-Sheng |
| 作者单位 | 1.Chinese Acad Sci, Chongqing Inst Green & Intelligent Technol, Chongqing 400714, Peoples R China 2.South China Agr Univ, Coll Marine Sci, Guangzhou 510642, Guangdong, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Shantou Univ, Coll Med, Ctr Neurosci, Shantou 515041, Peoples R China 5.Chinese Acad Sci, Guangzhou Inst Geochem, State Key Lab Organ Geochem, Guangzhou 510640, Guangdong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xie, Shao-Lin,Junaid, Muhammad,Bian, Wan-Ping,et al. Generation and application of a novel transgenic zebrafish line Tg(cyp1a:mCherry) as an in vivo assay to sensitively monitor PAHs and TCDD in the environment[J]. JOURNAL OF HAZARDOUS MATERIALS,2018,344:723-732. |
| APA | Xie, Shao-Lin.,Junaid, Muhammad.,Bian, Wan-Ping.,Luo, Juan-Juan.,Syed, Jabir Hussain.,...&Pei, De-Sheng.(2018).Generation and application of a novel transgenic zebrafish line Tg(cyp1a:mCherry) as an in vivo assay to sensitively monitor PAHs and TCDD in the environment.JOURNAL OF HAZARDOUS MATERIALS,344,723-732. |
| MLA | Xie, Shao-Lin,et al."Generation and application of a novel transgenic zebrafish line Tg(cyp1a:mCherry) as an in vivo assay to sensitively monitor PAHs and TCDD in the environment".JOURNAL OF HAZARDOUS MATERIALS 344(2018):723-732. |
入库方式： OAI收割
来源：重庆绿色智能技术研究院
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