Reproductive effects linked to DNA methylation in male zebrafish chronically exposed to environmentally relevant concentrations of di-(2-ethylhexyl) phthalate
文献类型：期刊论文
| 作者 | Ma, Yan-Bo1,2 ; Jia, Pan-Pan1,2; Junaid, Muhammad1,2; Yang, Li1; Lu, Chun-Jiao1; Pei, De-Sheng1,2
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| 刊名 | ENVIRONMENTAL POLLUTION
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| 出版日期 | 2018-06-01 |
| 卷号 | 237页码:1050-1061 |
| ISSN号 | 0269-7491 |
| 关键词 | Di-(2-ethylhexyl) phthalate DNA methylation Male zebrafish Reproduction toxicity |
| DOI | 10.1016/j.envpol.2017.11.025 |
| 英文摘要 | Di-(2-ethylhexyl) phthalate (DEHP) possesses the potential to interfere with the male reproductive endocrine system in mammals; however, its reproductive toxicity in male zebrafish and associated epigenetic studies have not been explored. In this study, three-month-old male zebrafish were exposed to environmentally relevant concentrations of DEHP (0, 10, 33 and 100 mu g/L for 3 months, and then the impact on the reproduction of males and the underlying mechanism were investigated. Histological testing showed that an exposure concentration of 100 mu g/L DEHP significantly inhibited spermatogenesis, with an associated decline in capability to fertilize untreated oocytes. Electron microscopic examinations also revealed noticeable damage to the testicular ultrastructure at the 100 mu g/L DEHP exposure level. In addition, exposure to 33 and 100 mu g/L of DEHP resulted in a decline of circulating testosterone (T) and an increase in the level of 17 beta-estradiol (E2), both of which were possibly derived from the downregulation of cypl7a1 and hsd1763 genes and the upregulation of the cyp19a1a gene in the gonads. The DNA methylation statuses of these genes were altered within their promoter regions. A significant increase in global DNA methylation in both the male testes and their offspring larvae was observed at higher exposure concentration of DEHP. Our findings demonstrate that exposure to environmentally relevant concentrations of DEHP can damage the testes, disturbe the sex hormones production, and inhibite spermatogenesis, which ultimately impairs the reproduction of male zebrafish. (C) 2017 Elsevier Ltd. All rights reserved. |
| 资助项目 | National Natural Science Foundation of China[21407146] ; Three Hundred Leading Talents in Scientific and Technological Innovation Program of Chongqing[CSTCCXLJRC201714] ; Key Application and Development Program of Chongqing Science and Technology Commission[cstc2014yykfC20004] ; Key Application and Development Program of Chongqing Science and Technology Commission[cstc2014yykfC20002] ; Chongqing research program of basic research and frontier technology[cstc2017jcyjAX0272] ; West Light PhD Foundation of the Chinese Academy of Sciences |
| WOS研究方向 | Environmental Sciences & Ecology |
| 语种 | 英语 |
| 出版者 | ELSEVIER SCI LTD |
| WOS记录号 | WOS:000431158900107 |
| 源URL | [http://119.78.100.138/handle/2HOD01W0/6357]  |
| 专题 | 环境与健康研究中心 |
| 通讯作者 | Pei, De-Sheng |
| 作者单位 | 1.Chinese Acad Sci, Chongqing Inst Green & Intelligent Technol, Chongqing 400714, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ma, Yan-Bo,Jia, Pan-Pan,Junaid, Muhammad,et al. Reproductive effects linked to DNA methylation in male zebrafish chronically exposed to environmentally relevant concentrations of di-(2-ethylhexyl) phthalate[J]. ENVIRONMENTAL POLLUTION,2018,237:1050-1061. |
| APA | Ma, Yan-Bo,Jia, Pan-Pan,Junaid, Muhammad,Yang, Li,Lu, Chun-Jiao,&Pei, De-Sheng.(2018).Reproductive effects linked to DNA methylation in male zebrafish chronically exposed to environmentally relevant concentrations of di-(2-ethylhexyl) phthalate.ENVIRONMENTAL POLLUTION,237,1050-1061. |
| MLA | Ma, Yan-Bo,et al."Reproductive effects linked to DNA methylation in male zebrafish chronically exposed to environmentally relevant concentrations of di-(2-ethylhexyl) phthalate".ENVIRONMENTAL POLLUTION 237(2018):1050-1061. |
入库方式： OAI收割
来源：重庆绿色智能技术研究院
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