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Occurrence State and Molecular Structure Analysis of Extracellular Proteins with Implications on the Dewaterability of Waste-Activated Sludge

文献类型：期刊论文

作者Wu, Boran1; Ni, Bing-Jie1; Horvat, Kristine2; Song, Liyan3; Chai, Xiaoli1; Dai, Xiaohu1; Mahajan, Devinder2
刊名ENVIRONMENTAL SCIENCE & TECHNOLOGY
出版日期2017-08-15
卷号51期号:16页码:9235-9243
ISSN号0013-936X
DOI10.1021/acs.est.7b02861
通讯作者Dai, XH (reprint author), Tongji Univ, Coll Environm Sci & Engn, State Key Lab Pollut Control & Resource Reuse, 1239 Siping Rd, Shanghai 200092, Peoples R China.
英文摘要The occurrence state and molecular structure of extracellular proteins were analyzed to reveal the influencing factors on the water-holding capacities of protein-like substances in waste-activated sludge (WAS). The gelation process of extracellular proteins verified that advanced oxidation processes (AOPs) for WAS dewaterability improvement eliminated the water affinity of exttacellular proteins and prevented these macromolecules from forming stable colloidal aggregates. Isobaric tags for relative and absolute quantitation proteomics identified that most of the extracellular proteins were originally derived from the intracellular part and the proteins originally located in the extracellular part were mainly membrane-associated. The main mechanism of extracellular protein transformation during AOPs could be represented by the damage of the membrane or related external encapsulating structure and the release of intracellular substances. For the selected representative extracellular proteins, the strong correlation (R-2 > 0.97, p < 0.03) between the surface hydrophilicity index and alpha-helix percentages in the secondary structure indicated that the water affinity relied more on the spatial distribution of hydrophilic functional groups rather than the content. Destructing the secondary structure represented by the alpha-helix and stretching the polypeptide aggregation in the water phase through disulfide bond removal might be the key to eliminating the inhibitory effects of extracellular proteins on the interstitial water removal from WAS.
资助项目Ministry of Science and Technology, People's Republic of China[2013ZX07315-002] ; International Exchange Program for Graduate Students, Tongji University[2016020009]
WOS研究方向Engineering ; Environmental Sciences & Ecology
语种英语
WOS记录号WOS:000407987400039
出版者AMER CHEMICAL SOC
源URL[http://172.16.51.4:88/handle/2HOD01W0/173]  
专题中国科学院重庆绿色智能技术研究院
通讯作者Dai, Xiaohu
作者单位1.Tongji Univ, Coll Environm Sci & Engn, State Key Lab Pollut Control & Resource Reuse, 1239 Siping Rd, Shanghai 200092, Peoples R China
2.SUNY Stony Brook, Coll Engn & Appl Sci, 100 Nicolls Rd, Stony Brook, NY 11794 USA
3.Chinese Acad Sci, Chongqing Inst Green & Intelligent Technol, Environm Microbiol & Ecol Res Ctr, 266 Fangzheng Ave, Chongqing 400714, Peoples R China
推荐引用方式
GB/T 7714		 Wu, Boran,Ni, Bing-Jie,Horvat, Kristine,et al. Occurrence State and Molecular Structure Analysis of Extracellular Proteins with Implications on the Dewaterability of Waste-Activated Sludge[J]. ENVIRONMENTAL SCIENCE & TECHNOLOGY,2017,51(16):9235-9243.
APA Wu, Boran.,Ni, Bing-Jie.,Horvat, Kristine.,Song, Liyan.,Chai, Xiaoli.,...&Mahajan, Devinder.(2017).Occurrence State and Molecular Structure Analysis of Extracellular Proteins with Implications on the Dewaterability of Waste-Activated Sludge.ENVIRONMENTAL SCIENCE & TECHNOLOGY,51(16),9235-9243.
MLA Wu, Boran,et al."Occurrence State and Molecular Structure Analysis of Extracellular Proteins with Implications on the Dewaterability of Waste-Activated Sludge".ENVIRONMENTAL SCIENCE & TECHNOLOGY 51.16(2017):9235-9243.

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来源：重庆绿色智能技术研究院

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