Exploring the potential of iTRAQ proteomics for tracking the transformation of extracellular proteins from enzyme-disintegrated waste activated sludge
文献类型：期刊论文
| 作者 | Wu, Boran1; Su, Lianghu2; Song, Liyan3 ; Dai, Xiaohu1; Chai, Xiaoli1
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| 刊名 | BIORESOURCE TECHNOLOGY
 |
| 出版日期 | 2017-02-01 |
| 卷号 | 225页码:75-83 |
| 关键词 | Waste activated sludge Extracellular polymeric substances (EPS) iTRAQ Proteomics |
| ISSN号 | 0960-8524 |
| DOI | 10.1016/j.biortech.2016.11.041 |
| 通讯作者 | Chai, XL (reprint author), Tongji Univ, State Key Lab Pollut Control & Resource Reuse, Coll Environm Sci & Engn, 1239 Siping Rd, Shanghai 200092, Peoples R China. |
| 英文摘要 | To characterize the transformation of extracellular proteins extracted from raw and enzymedisintegrated waste activated sludge (WAS), extracts of extracellular polymeric substances (EPS) were subject to isobaric tags for relative and absolute quantitation (iTRAQ) proteomics analysis. 209 proteins were identified and categorized into three Gene Ontology classifications: "cellular components", "molecular function", and "biological processes". Most identified proteins originated from intracellular components, organelles, or cytoplasm, suggesting that cell decline and lysis represent the main sources of extracellular proteins in WAS. The major protein functions comprised "transporter binding activity", "macromolecule metabolic process", and "biosynthesis enzyme catalytic activity". In total, 15 proteins categorized as "membrane part" and "biological adhesion" and 10 as "organelle" were down-or up-regulated, respectively, implying that the enzyme-disintegration mainly induced WAS floc-structure disintegration via membrane structure and corresponding biological adhesion disruption. The proteomics study will provide valuable clues to better understand EPS changes associated with enzymatic treatment at molecular levels. (C) 2016 Elsevier Ltd. All rights reserved. |
| 资助项目 | Ministry of Science and Technology, People's Republic of China[2013ZX07315-002] |
| WOS研究方向 | Agriculture ; Biotechnology & Applied Microbiology ; Energy & Fuels |
| 语种 | 英语 |
| WOS记录号 | WOS:000395693700008 |
| 出版者 | ELSEVIER SCI LTD |
| 源URL | [http://172.16.51.4:88/handle/2HOD01W0/252]  |
| 专题 | 中国科学院重庆绿色智能技术研究院 |
| 通讯作者 | Chai, Xiaoli |
| 作者单位 | 1.Tongji Univ, State Key Lab Pollut Control & Resource Reuse, Coll Environm Sci & Engn, 1239 Siping Rd, Shanghai 200092, Peoples R China 2.Nanjing Inst Environm Sci, Minist Environm Protect, 8 Jiangwangmiao St, Nanjing 210042, Jiangsu, Peoples R China 3.Chinese Acad Sci, Chongqing Inst Green & Intelligent Technol, Environm Microbiol & Ecol Res Ctr, 266 Fangzheng Ave, Chongqing 400714, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wu, Boran,Su, Lianghu,Song, Liyan,et al. Exploring the potential of iTRAQ proteomics for tracking the transformation of extracellular proteins from enzyme-disintegrated waste activated sludge[J]. BIORESOURCE TECHNOLOGY,2017,225:75-83. |
| APA | Wu, Boran,Su, Lianghu,Song, Liyan,Dai, Xiaohu,&Chai, Xiaoli.(2017).Exploring the potential of iTRAQ proteomics for tracking the transformation of extracellular proteins from enzyme-disintegrated waste activated sludge.BIORESOURCE TECHNOLOGY,225,75-83. |
| MLA | Wu, Boran,et al."Exploring the potential of iTRAQ proteomics for tracking the transformation of extracellular proteins from enzyme-disintegrated waste activated sludge".BIORESOURCE TECHNOLOGY 225(2017):75-83. |
入库方式： OAI收割
来源：重庆绿色智能技术研究院
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