Mycothiol protects Corynebacterium glutamicum against acid stress via maintaining intracellular pH homeostasis, scavenging ROS, and S-mycothiolating MetE
文献类型：期刊论文
| 作者 | Liu, Yingbao1,2,4; Yang, Xiaobing1,2; Yin, Yajie5 ; Lin, Jinshui1,2; Chen, Can1,2; Pan, Junfeng1,2; Si, Meiru3; Shen, Xihui1,2
|
| 刊名 | JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY
 |
| 出版日期 | 2016 |
| 卷号 | 62期号:3页码:144-153 |
| 关键词 | acid stress Corynebacterium glutamicum mycothiol pH homeostasis S-mycothiolation |
| ISSN号 | 0022-1260 |
| DOI | 10.2323/jgam.2016.02.001 |
| 通讯作者 | Shen, XH (reprint author), Northwest A&F Univ, State Key Lab Crop Stress Biol Arid Areas, Yangling 712100, Shaanxi, Peoples R China. ; Si, MR (reprint author), Qufu Normal Univ, Coll Life Sci, Qufu 273165, Shandong, Peoples R China. |
| 英文摘要 | Mycothiol (MSH) plays a major role in protecting cells against oxidative stress and detoxification from a broad range of exogenous toxic agents. In the present study, we reveal that intracellular MSH contributes significantly to the adaptation, to acidic conditions in the model organism Corynebacterium glutamicum. We present evidence that MSH confers C. glutanzicum with the ability to adapt to acidic conditions by maintaining pH(i) homeostasis, scavenging reactive oxygen species (ROS), and protecting methionine synthesis by the S-mycothiolation modification of methionine synthase (MetE). The role of MSH in acid adaptation was further confirmed by improving the acid tolerance of C. glutamicum by overexpressing the key MSH synthesis gene mshA. Hence, our work provides insights into a previously unknown, but important, aspect of the C. glutamicum cellular response to acid stress. The results reported here may help to understand acid tolerance mechanisms in acid sensitive bacteria and may open a new avenue for improving acid resistance in industry strains for the production of bio-based chemicals from renewable biomass. |
| 资助项目 | National High Technology Research and Development Program of China (863 program)[2013AA102802] ; National Natural Science Foundation of China[31270078] ; National Natural Science Foundation of China[31170121] ; National Natural Science Foundation of China[31500087] ; Key Science and Technology R&D Program of Shaanxi Province, China[2014K02-12-01] ; Natural Science Foundation of Shandong Province, China[ZR2015CM012] |
| WOS研究方向 | Biotechnology & Applied Microbiology ; Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000381846300006 |
| 出版者 | MICROBIOL RES FOUNDATION |
| 源URL | [http://119.78.100.138/handle/2HOD01W0/2728]  |
| 专题 | 中国科学院重庆绿色智能技术研究院 |
| 通讯作者 | Si, Meiru; Shen, Xihui |
| 作者单位 | 1.Northwest A&F Univ, State Key Lab Crop Stress Biol Arid Areas, Yangling 712100, Shaanxi, Peoples R China 2.Northwest A&F Univ, Coll Life Sci, Yangling 712100, Shaanxi, Peoples R China 3.Qufu Normal Univ, Coll Life Sci, Qufu 273165, Shandong, Peoples R China 4.Yangtze Univ, Coll Life Sci, Jingzhou 434025, Hubei, Peoples R China 5.Chinese Acad Sci, Chongqing Inst Green & Intelligent Technol, Key Lab Reservoir Aquat Environm, Chongqing 400714, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Yingbao,Yang, Xiaobing,Yin, Yajie,et al. Mycothiol protects Corynebacterium glutamicum against acid stress via maintaining intracellular pH homeostasis, scavenging ROS, and S-mycothiolating MetE[J]. JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY,2016,62(3):144-153. |
| APA | Liu, Yingbao.,Yang, Xiaobing.,Yin, Yajie.,Lin, Jinshui.,Chen, Can.,...&Shen, Xihui.(2016).Mycothiol protects Corynebacterium glutamicum against acid stress via maintaining intracellular pH homeostasis, scavenging ROS, and S-mycothiolating MetE.JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY,62(3),144-153. |
| MLA | Liu, Yingbao,et al."Mycothiol protects Corynebacterium glutamicum against acid stress via maintaining intracellular pH homeostasis, scavenging ROS, and S-mycothiolating MetE".JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY 62.3(2016):144-153. |
入库方式： OAI收割
来源：重庆绿色智能技术研究院
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。