Application of Faecalibacterium 16S rDNA genetic marker for accurate identification of duck faeces
文献类型：期刊论文
| 作者 | Sun, Da1; Duan, Chuanren1; Shang, Yaning1; Ma, Yunxia1; Tan, Lili1; Zhai, Jun2; Gao, Xu2; Guo, Jingsong3 ; Wang, Guixue1
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| 刊名 | ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
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| 出版日期 | 2016-04-01 |
| 卷号 | 23期号:8页码:7639-7647 |
| 关键词 | Faecalibacterium Host-specific PCR assay Water pollution Duck |
| ISSN号 | 0944-1344 |
| DOI | 10.1007/s11356-015-6024-z |
| 通讯作者 | Duan, CR ; Wang, GX (reprint author), Chongqing Univ, State & Local Joint Engn Lab Vasc Implants Chongq, Bioengn Coll, Key Lab Biorheol Sci & Technol,Minist Educ, Chongqing 630044, Peoples R China. |
| 英文摘要 | The aim of this study was to judge the legal duty of pollution liabilities by assessing a duck faeces-specific marker, which can exclude distractions of residual bacteria from earlier contamination accidents. With the gene sequencing technology and bioinformatics method, we completed the comparative analysis of Faecalibacterium sequences, which were associated with ducks and other animal species, and found the sequences unique to duck faeces. Polymerase chain reaction (PCR) and agarose gel electrophoresis techniques were used to verify the reliability of both human and duck faeces-specific primers. The duck faeces-specific primers generated an amplicon of 141 bp from 43.3 % of duck faecal samples, 0 % of control samples and 100 % of sewage wastewater samples that contained duck faeces. We present here the initial evidence of Faecalibacterium-based applicability as human faeces-specificity in China. Meanwhile, this study represents the initial report of a Faecalibacterium marker for duck faeces and suggests an independent or supplementary environmental biotechnology of microbial source tracking (MST). |
| 资助项目 | National Science and Technology Supporting Program of China[2012BAJ25B06] ; National Science and Technology Supporting Program of China[2012BAJ25B09] ; National Natural Science Foundation of China[51208533] ; Fundamental Research Funds for the Central Universities[106112015CDJZR235504] ; National Water Pollution Control and Management Technology Major Projects of China[2008ZX07425-003-01] ; Chongqing Engineering Laboratory in Vascular Implants ; National '111 Plan' Base[B06023] ; Public Experiment Center of State Bioindustrial Base (Chongqing), China |
| WOS研究方向 | Environmental Sciences & Ecology |
| 语种 | 英语 |
| WOS记录号 | WOS:000374994600056 |
| 出版者 | SPRINGER HEIDELBERG |
| 源URL | [http://119.78.100.138/handle/2HOD01W0/2470]  |
| 专题 | 水质生物转化研究中心 |
| 通讯作者 | Duan, Chuanren; Wang, Guixue |
| 作者单位 | 1.Chongqing Univ, State & Local Joint Engn Lab Vasc Implants Chongq, Bioengn Coll, Key Lab Biorheol Sci & Technol,Minist Educ, Chongqing 630044, Peoples R China 2.Chongqing Univ, Key Lab Three Gorges Reservoir Reg Ecoenvironm, Coll Urban Construct & Environm Engn, Chongqing 630044, Peoples R China 3.Chinese Acad Sci, Key Lab Reservoir Aquat Environm CAS, Chongqing Inst Green & Intelligent Technol, Chongqing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Sun, Da,Duan, Chuanren,Shang, Yaning,et al. Application of Faecalibacterium 16S rDNA genetic marker for accurate identification of duck faeces[J]. ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH,2016,23(8):7639-7647. |
| APA | Sun, Da.,Duan, Chuanren.,Shang, Yaning.,Ma, Yunxia.,Tan, Lili.,...&Wang, Guixue.(2016).Application of Faecalibacterium 16S rDNA genetic marker for accurate identification of duck faeces.ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH,23(8),7639-7647. |
| MLA | Sun, Da,et al."Application of Faecalibacterium 16S rDNA genetic marker for accurate identification of duck faeces".ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH 23.8(2016):7639-7647. |
入库方式： OAI收割
来源：重庆绿色智能技术研究院
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