Distribution and transcription activity of nucleolar DNA in higher plant cells
文献类型：期刊论文
| 作者 | Tao, W; Xu, W; Valdivia, MM; Hao, S; Zhai, ZH; W. Tao |
| 刊名 | CELL BIOLOGY INTERNATIONAL
 |
| 出版日期 | 2001 |
| 卷号 | 25期号:11页码:1167-1171 |
| 关键词 | nucleolus location of nucleolar DNA in situ transcription sites of rRNA genes RNA polymerase I transcription factor (UBF) Alllium cepa |
| ISSN号 | 1065-6995 |
| 文献子类 | Article |
| 英文摘要 | By using the NAMA-Ur DNA selective staining method, we have observed in situ the location of nucleolar DNA in onion cells and found it at the boundary between fibrillar centres (FC) and dense fibrillar component (DFC) in transcriptionally active nucleolus. We have also used anti-NOR serum, which is identified as the RNA Polymerase I transcription factor (UBF) antibody, to study its reactivity with higher plant cells and demonstrated this factor associated to the DFC but not present at the interior of FC. Finally, by employing anti-DNA/RNA hybrid antibodies, we labeled the transcriptionally active rRNA genes in active nucleolus and testified that at the boundary between FC and DFC. The results provide the evidence that the boundary between FC and DFC is the genuine transcription site of rRNA genes in nucleolus.; By using the NAMA-Ur DNA selective staining method, we have observed in situ the location of nucleolar DNA in onion cells and found it at the boundary between fibrillar centres (FC) and dense fibrillar component (DFC) in transcriptionally active nucleolus. We have also used anti-NOR serum, which is identified as the RNA Polymerase I transcription factor (UBF) antibody, to study its reactivity with higher plant cells and demonstrated this factor associated to the DFC but not present at the interior of FC. Finally, by employing anti-DNA/RNA hybrid antibodies, we labeled the transcriptionally active rRNA genes in active nucleolus and testified that at the boundary between FC and DFC. The results provide the evidence that the boundary between FC and DFC is the genuine transcription site of rRNA genes in nucleolus. (C) 2001 Academic Press. |
| 学科主题 | 生物学 |
| 语种 | 英语 |
| WOS记录号 | WOS:000172524000011 |
| 公开日期 | 2011-08-22 |
| 源URL | [http://ir.psych.ac.cn/handle/311026/5323]  |
| 专题 | 心理研究所_中国科学院心理研究所回溯数据库(1956-2010) |
| 通讯作者 | W. Tao |
| 作者单位 | 1.Peking Univ, Coll Life Sci, Dept Cell Biol, Sch Life Sci, Beijing 100871, Peoples R China 2.NE Normal Univ, Inst Cytol & Genet, Changchun 130024, Jiling, Peoples R China 3.Univ Cadiz, Fac Sci, Dept Biochem & Mol Biol, Cadiz 11510, Spain 4.Acad Sinica, Inst Psychol, Dept Med Physiol & Psychol, Beijing 100101, Peoples R China |
| 推荐引用方式 GB/T 7714 | Tao, W,Xu, W,Valdivia, MM,et al. Distribution and transcription activity of nucleolar DNA in higher plant cells[J]. CELL BIOLOGY INTERNATIONAL,2001,25(11):1167-1171. |
| APA | Tao, W,Xu, W,Valdivia, MM,Hao, S,Zhai, ZH,&W. Tao.(2001).Distribution and transcription activity of nucleolar DNA in higher plant cells.CELL BIOLOGY INTERNATIONAL,25(11),1167-1171. |
| MLA | Tao, W,et al."Distribution and transcription activity of nucleolar DNA in higher plant cells".CELL BIOLOGY INTERNATIONAL 25.11(2001):1167-1171. |
入库方式： OAI收割
来源：心理研究所
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