Proteomic analysis of rat prefrontal cortex in three phases of morphine-induced conditioned place preference
文献类型：期刊论文
| 作者 | Yang, Liu; Sun, Zhong Sheng; Zhu, Yong-ping; Y. P. Zhu |
| 刊名 | JOURNAL OF PROTEOME RESEARCH
 |
| 出版日期 | 2007 |
| 卷号 | 6期号:6页码:2239-2247 |
| 关键词 | morphine addiction conditioned place preference (CPP) prefrontal cortex (PFC) proteomics neuroplasticity |
| ISSN号 | 1535-3893 |
| 文献子类 | Article |
| 英文摘要 | Morphological alterations of synapse are found after morphine administration, suggesting that regulation of synaptic plasticity may be one of the mechanisms of neuroadaptation in addiction. However, the molecular basis underlying the abnormal synapse morphological and physiological changes in the morphine-induced dependence, withdraw, and relapse is not well understood. As prefrontal cortex ( PFC) is one of the most important brain regions, which provides executive control over drug use and is severely impaired in many addicts, systematic analysis of the biochemical and molecular alteration of synaptic fraction of PFC in morphine-induced neuroadaptation is necessary. In this study, differential protein expression profiling of synaptic fraction of rat PFC based on morphine-induced conditioned place preference ( CPP) model was performed with two-dimensional gel electrophoresis ( 2-DE). Our results showed that a total of 80 proteins were differentially expressed by 2-DE analysis during three phases of CPP assay. Of them, 58 were further identified by mass spectrometry. These proteins were classified into multiple categories, such as energy metabolism, signal transduction, synaptic transmission, cytoskeletal proteins, chaperones, and local synaptic protein synthetic machinery according to their biological functions. Our study provides a global view of synaptic-related molecular networking in PFC under morphine-induced dependence, withdraw, and relapse, indicative of a concerted biological process in neuroadaptation under chronic morphine exposure.; Morphological alterations of synapse are found after morphine administration, suggesting that regulation of synaptic plasticity may be one of the mechanisms of neuroadaptation in addiction. However, the molecular basis underlying the abnormal synapse morphological and physiological changes in the morphine-induced dependence, withdraw, and relapse is not well understood. As prefrontal cortex ( PFC) is one of the most important brain regions, which provides executive control over drug use and is severely impaired in many addicts, systematic analysis of the biochemical and molecular alteration of synaptic fraction of PFC in morphine-induced neuroadaptation is necessary. In this study, differential protein expression profiling of synaptic fraction of rat PFC based on morphine-induced conditioned place preference ( CPP) model was performed with two-dimensional gel electrophoresis ( 2-DE). Our results showed that a total of 80 proteins were differentially expressed by 2-DE analysis during three phases of CPP assay. Of them, 58 were further identified by mass spectrometry. These proteins were classified into multiple categories, such as energy metabolism, signal transduction, synaptic transmission, cytoskeletal proteins, chaperones, and local synaptic protein synthetic machinery according to their biological functions. Our study provides a global view of synaptic-related molecular networking in PFC under morphine-induced dependence, withdraw, and relapse, indicative of a concerted biological process in neuroadaptation under chronic morphine exposure. |
| 学科主题 | 医学心理学 |
| 语种 | 英语 |
| WOS记录号 | WOS:000246893500020 |
| 公开日期 | 2011-08-22 |
| 源URL | [http://ir.psych.ac.cn/handle/311026/5639]  |
| 专题 | 心理研究所_中国科学院心理研究所回溯数据库(1956-2010) |
| 通讯作者 | Y. P. Zhu |
| 作者单位 | 1.Zhejiang Univ, Sch Med, Dept Toxicol, Hangzhou, Zhejiang, Peoples R China 2.Zhejiang Univ, James D Watson Inst Genome Sci, Hangzhou, Zhejiang, Peoples R China 3.Chinese Acad Sci, Inst Psychol, Behav Genet Ctr, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yang, Liu,Sun, Zhong Sheng,Zhu, Yong-ping,et al. Proteomic analysis of rat prefrontal cortex in three phases of morphine-induced conditioned place preference[J]. JOURNAL OF PROTEOME RESEARCH,2007,6(6):2239-2247. |
| APA | Yang, Liu,Sun, Zhong Sheng,Zhu, Yong-ping,&Y. P. Zhu.(2007).Proteomic analysis of rat prefrontal cortex in three phases of morphine-induced conditioned place preference.JOURNAL OF PROTEOME RESEARCH,6(6),2239-2247. |
| MLA | Yang, Liu,et al."Proteomic analysis of rat prefrontal cortex in three phases of morphine-induced conditioned place preference".JOURNAL OF PROTEOME RESEARCH 6.6(2007):2239-2247. |
入库方式： OAI收割
来源：心理研究所
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