Morphine induces Beclin 1-and ATG5-dependent autophagy in human neuroblastoma SH-SY5Y cells and in the rat hippocampus
文献类型：期刊论文
| 作者 | Zhao, Lixia1,2; Zhu, Yushan1,3; Wang, Dongmei4 ; Chen, Ming1; Gao, Ping1; Xiao, Weiming5; Rao, Guanhua3; Wang, Xiaohui1; Jin, Haijing1; Xu, Lin6
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| 刊名 | AUTOPHAGY
 |
| 出版日期 | 2010-04-01 |
| 卷号 | 6期号:3页码:386-394 |
| 关键词 | morphine autophagy cell death Beclin 1 ATG5 |
| ISSN号 | 1554-8627 |
| 文献子类 | Article |
| 英文摘要 | Chronic exposure to morphine can induce drug addiction and neural injury, but the exact mechanism is not fully understood. Here we show that morphine induces autophagy in neuroblastoma SH-SY5Y cells and in the rat hippocampus. Pharmacological approach shows that this effect appears to be mediated by PTX-sensitive G protein-coupled receptors signaling cascade. Morphine increases Beclin 1 expression and reduces the interaction between Beclin 1 and Bcl-2, thus releasing Beclin 1 for its pro-autophagic activity. Bcl-2 overexpression inhibits morphine-induced autophagy, whereas knockdown of Beclin 1 or knockout of ATG5 prevents morphine-induced autophagy. In addition, chronic treatment with morphine induces cell death, which is increased by autophagy inhibition through Beclin 1 RNAi. Our data are the first to reveal that Beclin 1 and ATG5 play key roles in morphine-induced autophagy, which may contribute to morphine-induced neuronal injury. |
| 学科主题 | 医学心理学 |
| 资助项目 | 一室隋南课题组 |
| 语种 | 英语 |
| WOS记录号 | WOS:000276198200009 |
| 公开日期 | 2011-11-14 |
| 源URL | [http://ir.psych.ac.cn/handle/311026/7033]  |
| 专题 | 心理研究所_中国科学院心理研究所回溯数据库(1956-2010) |
| 通讯作者 | Sui N(隋南) |
| 作者单位 | 1.Chinese Acad Sci, Inst Zool, State Key Lab Biomembrane & Membrane Biotechnol, Lab Apoptosis & Canc Biol, Beijing 100864, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100864, Peoples R China 3.Nankai Univ, Coll Life Sci, Tianjin, Peoples R China 4.Chinese Acad Sci, Inst Psychol, Key Lab Mental Hlth, Beijing 100864, Peoples R China 5.Peking Univ, Coll Life Sci, Beijing, Peoples R China 6.Chinese Acad Sci, Kunming Inst Zool, Key Lab Anim Models & Human Dis Mech, Kunming, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao, Lixia,Zhu, Yushan,Wang, Dongmei,et al. Morphine induces Beclin 1-and ATG5-dependent autophagy in human neuroblastoma SH-SY5Y cells and in the rat hippocampus[J]. AUTOPHAGY,2010,6(3):386-394. |
| APA | Zhao, Lixia.,Zhu, Yushan.,Wang, Dongmei.,Chen, Ming.,Gao, Ping.,...&隋南.(2010).Morphine induces Beclin 1-and ATG5-dependent autophagy in human neuroblastoma SH-SY5Y cells and in the rat hippocampus.AUTOPHAGY,6(3),386-394. |
| MLA | Zhao, Lixia,et al."Morphine induces Beclin 1-and ATG5-dependent autophagy in human neuroblastoma SH-SY5Y cells and in the rat hippocampus".AUTOPHAGY 6.3(2010):386-394. |
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来源：心理研究所
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