Optimization of Zygosity Determination by Questionnaire and DNA Genotyping in Chinese Adolescent Twins
文献类型：期刊论文
| 作者 | Chen, Jie1 ; Li, Xinying1; Chen, Zhiyan1; Yang, Xiaodong1; Zhang, Jie1; Duan, Qing1; Ge, Xiaojia1,2; Li XY(李新影)
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| 刊名 | TWIN RESEARCH AND HUMAN GENETICS
 |
| 出版日期 | 2010-04-01 |
| 卷号 | 13期号:2页码:194-200 |
| 关键词 | zygosity determination in Chinese adolescent twins |
| ISSN号 | 1832-4274 |
| 文献子类 | Article |
| 英文摘要 | The main aim of this study was to develop and optimize a questionnaire-based zygosity determination method in Chinese adolescent twins. Participants were 471 pairs of same-sex twins (345 monozygotic, 126 dizygotic) with a mean age of 14.56 years (SD = 2.62). A second sample was recruited for cross-validation, including 382 pairs of same-sex twins (261 monozygotic, 121 dizygotic) with a mean age of 12.53 years (SD = 2.22). The questionnaire consisted of 12 questions dealing with co-twin similarity or frequency of confusion. Two means were put forward to improve the predictive accuracy of the questionnaire adding parent-reports to the analysis, and using a 2-point rather than 3-point response format. DNA genotyping was performed on nine short tandem repeat loci, with an estimated zygosity classification accuracy very close to 100%. The validity of all questionnaires was assessed by being compared to the results of DNA analysis. Results of stepwise logistic regression analysis showed that the predictive accuracy of the 3-point self-reported questionnaire is 83.8%. Using parent-reports and 2-point scale led to 3.9% and 4.6% increase in predictive accuracy, respectively. When using the parent-reports and children's self-reports jointly, the predictive accuracy was enhanced to 90.6%. For the cross-validation, the equations and cut-offs derived from the first sample led to an acceptable accuracy (91.3%) in the second sample. In conclusion, the method we developed can be used in future studies among Chinese adolescent twins. Multiple-rater and 2-point response format were suggested for all twin studies for enhancing the predictive accuracy of questionnaires. |
| 学科主题 | 心理测量学 |
| 资助项目 | 一室李新颖课题组 |
| 语种 | 英语 |
| WOS记录号 | WOS:000287994500008 |
| 公开日期 | 2011-12-02 |
| 源URL | [http://ir.psych.ac.cn/handle/311026/7317]  |
| 专题 | 心理研究所_中国科学院心理研究所回溯数据库(1956-2010) |
| 通讯作者 | Li XY(李新影) |
| 作者单位 | 1.Chinese Acad Sci, Inst Psychol, Key Lab Mental Hlth, Beijing 100101, Peoples R China 2.Univ Minnesota Twin Cities, Inst Child Dev, Minneapolis, MN 55455 USA |
| 推荐引用方式 GB/T 7714 | Chen, Jie,Li, Xinying,Chen, Zhiyan,et al. Optimization of Zygosity Determination by Questionnaire and DNA Genotyping in Chinese Adolescent Twins[J]. TWIN RESEARCH AND HUMAN GENETICS,2010,13(2):194-200. |
| APA | Chen, Jie.,Li, Xinying.,Chen, Zhiyan.,Yang, Xiaodong.,Zhang, Jie.,...&李新影.(2010).Optimization of Zygosity Determination by Questionnaire and DNA Genotyping in Chinese Adolescent Twins.TWIN RESEARCH AND HUMAN GENETICS,13(2),194-200. |
| MLA | Chen, Jie,et al."Optimization of Zygosity Determination by Questionnaire and DNA Genotyping in Chinese Adolescent Twins".TWIN RESEARCH AND HUMAN GENETICS 13.2(2010):194-200. |
入库方式： OAI收割
来源：心理研究所
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