Gene expression of conditioned locomotion and context-specific locomotor sensitization controlled by morphine-associated environment
文献类型：期刊论文
| 作者 | Liang, Jing1 ; Chen, Ji-Huan2; Chen, Xiao-Hong3; Peng, Yong-Hua1; Zheng, Xi-Geng1; Zheng, XG (reprint author), Chinese Acad Sci, Inst Psychol, Key Lab Mental Hlth, Beijing 100101, Peoples R China.; Zheng, Xigeng
|
| 刊名 | BEHAVIOURAL BRAIN RESEARCH
 |
| 出版日期 | 2011 |
| 卷号 | 216期号:1页码:321-331 |
| 关键词 | Morphine Conditioned response Sensitization Nucleus accumbens Microarray |
| ISSN号 | 0166-4328 |
| 产权排序 | 1 |
| 文献子类 | Article |
| 英文摘要 | The nucleus accumbens (NAc) is involved in contextual drug associations, which might be particularly important for environmental cue-induced relapse to drug seeking. In the present study, rats were first administered repeated morphine for 5 days (5 mg/kg, i.p.) in a contextually paired and unpaired design. After reexposure to the morphine-associated environment, which induced conditioned locomotor activity in the morphine-paired group, we performed a rat 27k 70-mer oligo array to profile gene expression in the NAc. One hundred fifty-five upregulated and 88 downregulated genes were found in the paired group compared with the unpaired group. Eight gene transcripts were then selected to confirm their alterations by quantitative real-time polymerase chain reaction (qRT-PCR). The identified genes generally play important roles in neuroactive receptor-ligand interactions, synapse plasticity, ion transport, and protein phosphorylation. Furthermore, the expression of the eight selected genes that were identified and confirmed to show significant fold changes in the first microarray experiment were again measured with qRT-PCR after morphine challenge (2 mg/kg, i.p.). As expected, 2 mg/kg morphine-induced context-specific sensitization. Meanwhile, mRNA expression of the selected genes showed marked upregulation in the morphine-paired group compared with the unpaired and acute groups. These results suggest that alterations in the expression of the identified genes in the NAc may contribute to the neuroplasticity underlying contextual cue-induced relapse to drug use. (C) 2010 Elsevier B.V. All rights reserved. |
| 学科主题 | Cognitive Neuroscience |
| URL标识 | 查看原文 |
| 资助项目 | 一室郑希耕课题组 |
| 语种 | 英语 |
| WOS记录号 | WOS:000285318500044 |
| 资助机构 | National Foundation of Natural Science [30470578, 30900400, 30770722] ; Institute of Psychology, Chinese Academy of Sciences [08CX053005] |
| 公开日期 | 2011-11-11 |
| 源URL | [http://ir.psych.ac.cn/handle/311026/6917]  |
| 专题 | 心理研究所_健康与遗传心理学研究室 |
| 通讯作者 | Zheng, XG (reprint author), Chinese Acad Sci, Inst Psychol, Key Lab Mental Hlth, Beijing 100101, Peoples R China.; Zheng, Xigeng |
| 作者单位 | 1.Chinese Acad Sci, Inst Psychol, Key Lab Mental Hlth, Beijing 100101, Peoples R China 2.Scripps Res Inst, Mol & Integrat Neurosci Dept, La Jolla, CA 92037 USA 3.CapitalBio Corp, Beijing 102206, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liang, Jing,Chen, Ji-Huan,Chen, Xiao-Hong,et al. Gene expression of conditioned locomotion and context-specific locomotor sensitization controlled by morphine-associated environment[J]. BEHAVIOURAL BRAIN RESEARCH,2011,216(1):321-331. |
| APA | Liang, Jing.,Chen, Ji-Huan.,Chen, Xiao-Hong.,Peng, Yong-Hua.,Zheng, Xi-Geng.,...&Zheng, Xigeng.(2011).Gene expression of conditioned locomotion and context-specific locomotor sensitization controlled by morphine-associated environment.BEHAVIOURAL BRAIN RESEARCH,216(1),321-331. |
| MLA | Liang, Jing,et al."Gene expression of conditioned locomotion and context-specific locomotor sensitization controlled by morphine-associated environment".BEHAVIOURAL BRAIN RESEARCH 216.1(2011):321-331. |
入库方式： OAI收割
来源：心理研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。